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EGFR<br />
EGF <br />
EGFR <br />
EGFR <br />
EGFR -<br />
-<br />
AP1 GGA <br />
EGFR <br />
AP1 GGA2 <br />
EGFR GGA1 <br />
GGA3 <br />
AP1 GGA2 <br />
EGFR <br />
D EGFR GGA2<br />
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CIMPR EGFR <br />
AP1•GGA2 EGFR EGFR <br />
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VAMP <br />
Khairani Astrid Feinisa <br />
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SNARE <br />
VAMP <br />
vSNARE VAMP5 mRNA <br />
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VAMP5 <br />
VAMP5 <br />
VAMP5 <br />
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VAMP5 <br />
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A23187: f6 μM Zymosan <br />
5 ms/frame <br />
AQUACOSMOS <br />
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1 <br />
47 <br />
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23 ms n=41<br />
18 ms n=21 <br />
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Involvement of Rab in phagosome formation through regulating<br />
ARF activity by ACAP<br />
1 2 1<br />
1<br />
2 <br />
Phagosome formation and subsequent maturation are complex sequences of<br />
events that involve actin cytoskeleton remodeling and membrane trafficking. In<br />
our livecell studies, we found that Rab35 accumulated markedly at the membrane<br />
where IgG opsonized erythrocytes IgGEs are bound. Rab35 silencing by RNA<br />
interference or the expression of GDP or GTPlocked Rab35 mutant drastically<br />
reduced the rate of phagocytosis of IgGEs. Actinmediated pseudopod extension<br />
to form phagocytic cups was disturbed by the Rab35 silencing or the expression<br />
of GDPRab35, although initial actin assembly at the IgGE binding sites was<br />
not inhibited. Furthermore, GTPRab35dependent recruitment of ACAP2, an<br />
ARF6 GAP, was shown in the phagocytic cup formation. The ARF6 activation<br />
during FcγRmediated phagocytosis was severely impaired by expressing GTP<br />
bound mutant of Rab35. Concomitantly, overexpression of ACAP2 along with<br />
GTPlocked Rab35 showed a synergistic inhibitory effect on phagocytosis. Taken<br />
together, it is likely that Rab35 regulates actindependent phagosome formation<br />
by recruiting ACAP2, which might control actin remodeling through ARF6.<br />
P<br />
AtgAmRNA HeLa /A study on<br />
AtgAmRNAknockdown HeLa cells<br />
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Atg9 <br />
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Atg9A 9B <br />
Atg9A <br />
HeLa <br />
Atg9AmRNA siRNA <br />
lamp1 <br />
<br />
<br />
EEA1 <br />
<br />
<br />
<br />
Atg9A