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118<br />
117 <br />
OGPMIV<br />
<br />
<br />
1 1 1,2 1,3 1,4<br />
1<br />
2 3 <br />
4 <br />
CS<br />
<br />
<br />
CS <br />
<br />
IgG <br />
IgM<br />
CS <br />
IgM<br />
IgM Hierck 1994<br />
CS IgM<br />
CS<br />
ABC <br />
CS <br />
CSA CSD CS56 <br />
<br />
IgM <br />
<br />
OGPMV<br />
Hes <br />
<br />
1 2 3 3<br />
1<br />
2 3 <br />
<br />
NotchHes <br />
Hes1 /<br />
Hes1/ <br />
SCG <br />
12.5 E12.5 E13.5 SCG <br />
E14.5 C1C3 <br />
Hes1/ E13.5 SCG <br />
E17.5 SCG <br />
26.3% <br />
SCG E13.0 3 <br />
SCG E13.5 SCG <br />
Hes/ <br />
E17.5 <br />
52.5% H3 SCG <br />
Hes1/ <br />
Hes1 SCG <br />
<br />
OGPMV<br />
M. iliotibialis cranialis<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
M.iliotibialis cranialis<br />
<br />
E5 <br />
<br />
E6 E7 <br />
E8 <br />
E9 L1<br />
L2 <br />
E5 <br />
<br />
<br />
<br />
OGPMV<br />
<br />
<br />
1 2 2 2<br />
1<br />
2 <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
CL57BL/68 <br />
50 mm 500 mm 0 45 90 <br />
<br />
BrdU 1 mg/ml 2 <br />
BrdU <br />
BrdU <br />
<br />
<br />
<br />
<br />
OGPMVI<br />
Differential effect of aberrant expression of ectodysplasinA receptor<br />
edar on scales and jaw and pharyngeal dentition of medaka<br />
Otto Baba 1 , ADSL Atukorala 1 , Keiji Inohara 2 , Makoto Tabata 1 , Kiyoshi Mitani 3 ,<br />
Yoshiro Takano 1<br />
1<br />
Biostructural Science, Graduate School, Tokyo Medical & Dental University,<br />
2<br />
Biological Information, Tokyo Institute of Technology, 3 Biological Science,<br />
Graduate School of Frontier Science, University of Tokyo<br />
To find out the role of ectodermal cell signaling in scale and tooth formation in<br />
teleosts and thereby to gain insights in evolutionary origin of teeth, we analyzed<br />
scales and teeth in rs3 medaka mutant characterized by reduced scale numbers<br />
due to aberrant splicing of ectodysplasinA receptor edar. Results: In normal<br />
medaka, we confirmed edar signals in the enamel epithelium at early tooth<br />
development in both jaw and pharyngeal dentition. The signal was gone once<br />
mineralized matrix had deposited. In adult rs3, drastic loss of scales 83 %<br />
and teeth occurred in both oral 43.5 % and pharyngeal 73.5 % dentition.<br />
Remaining scales were irregular and much larger in size relative to those of wild<br />
type. In contrast, there was no abnormality in size and shape in the remaining<br />
teeth of rs3. ThreeD analyses of embryonic development of pharyngeal regions<br />
indicated that pharyngeal tooth formation preceded gill slit opening and showed<br />
no sign of ectodermal cell migration in pharyngeal endoderm and hence no direct<br />
evidence of ectodermal contribution to pharyngeal odontogenesis. Conclusion:<br />
These data support intrinsic odontogenic competence of rostral endoderm in<br />
medaka.<br />
OGPMVI<br />
DNA TGFβ <br />
<br />
1 1 2 3<br />
1<br />
2 3 <br />
DNA <br />
RG108 MEE <br />
TGFβ3 KO <br />
<br />
RG108 ICR C57BL/6J MEE<br />
<br />
100 C57BL/6J <br />
TGFβ3KO RG108 <br />
<br />
in vitro ICR C57BL/6J <br />
MEE RG108 <br />
C57BL/6J MEE ICR <br />
C57BL6J TGFβ3KO <br />
RG108 <br />
ICR TGFβ3KO <br />
15.8<br />
<br />
DNA