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第117回日本解剖学会総会・全国学術集会 講演プログラム・抄録集 PDF ...

第117回日本解剖学会総会・全国学術集会 講演プログラム・抄録集 PDF ...

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118<br />

117 <br />

OGPMIV<br />

<br />

<br />

1 1 1,2 1,3 1,4<br />

1<br />

2 3 <br />

4 <br />

CS<br />

<br />

<br />

CS <br />

<br />

IgG <br />

IgM<br />

CS <br />

IgM<br />

IgM Hierck 1994<br />

CS IgM<br />

CS<br />

ABC <br />

CS <br />

CSA CSD CS56 <br />

<br />

IgM <br />

<br />

OGPMV<br />

Hes <br />

<br />

1 2 3 3<br />

1<br />

2 3 <br />

<br />

NotchHes <br />

Hes1 /<br />

Hes1/ <br />

SCG <br />

12.5 E12.5 E13.5 SCG <br />

E14.5 C1C3 <br />

Hes1/ E13.5 SCG <br />

E17.5 SCG <br />

26.3% <br />

SCG E13.0 3 <br />

SCG E13.5 SCG <br />

Hes/ <br />

E17.5 <br />

52.5% H3 SCG <br />

Hes1/ <br />

Hes1 SCG <br />

<br />

OGPMV<br />

M. iliotibialis cranialis<br />

<br />

<br />

<br />

<br />

<br />

<br />

<br />

M.iliotibialis cranialis<br />

<br />

E5 <br />

<br />

E6 E7 <br />

E8 <br />

E9 L1<br />

L2 <br />

E5 <br />

<br />

<br />

<br />

OGPMV<br />

<br />

<br />

1 2 2 2<br />

1<br />

2 <br />

<br />

<br />

<br />

<br />

<br />

<br />

<br />

CL57BL/68 <br />

50 mm 500 mm 0 45 90 <br />

<br />

BrdU 1 mg/ml 2 <br />

BrdU <br />

BrdU <br />

<br />

<br />

<br />

<br />

OGPMVI<br />

Differential effect of aberrant expression of ectodysplasinA receptor<br />

edar on scales and jaw and pharyngeal dentition of medaka<br />

Otto Baba 1 , ADSL Atukorala 1 , Keiji Inohara 2 , Makoto Tabata 1 , Kiyoshi Mitani 3 ,<br />

Yoshiro Takano 1<br />

1<br />

Biostructural Science, Graduate School, Tokyo Medical & Dental University,<br />

2<br />

Biological Information, Tokyo Institute of Technology, 3 Biological Science,<br />

Graduate School of Frontier Science, University of Tokyo<br />

To find out the role of ectodermal cell signaling in scale and tooth formation in<br />

teleosts and thereby to gain insights in evolutionary origin of teeth, we analyzed<br />

scales and teeth in rs3 medaka mutant characterized by reduced scale numbers<br />

due to aberrant splicing of ectodysplasinA receptor edar. Results: In normal<br />

medaka, we confirmed edar signals in the enamel epithelium at early tooth<br />

development in both jaw and pharyngeal dentition. The signal was gone once<br />

mineralized matrix had deposited. In adult rs3, drastic loss of scales 83 %<br />

and teeth occurred in both oral 43.5 % and pharyngeal 73.5 % dentition.<br />

Remaining scales were irregular and much larger in size relative to those of wild<br />

type. In contrast, there was no abnormality in size and shape in the remaining<br />

teeth of rs3. ThreeD analyses of embryonic development of pharyngeal regions<br />

indicated that pharyngeal tooth formation preceded gill slit opening and showed<br />

no sign of ectodermal cell migration in pharyngeal endoderm and hence no direct<br />

evidence of ectodermal contribution to pharyngeal odontogenesis. Conclusion:<br />

These data support intrinsic odontogenic competence of rostral endoderm in<br />

medaka.<br />

OGPMVI<br />

DNA TGFβ <br />

<br />

1 1 2 3<br />

1<br />

2 3 <br />

DNA <br />

RG108 MEE <br />

TGFβ3 KO <br />

<br />

RG108 ICR C57BL/6J MEE<br />

<br />

100 C57BL/6J <br />

TGFβ3KO RG108 <br />

<br />

in vitro ICR C57BL/6J <br />

MEE RG108 <br />

C57BL/6J MEE ICR <br />

C57BL6J TGFβ3KO <br />

RG108 <br />

ICR TGFβ3KO <br />

15.8<br />

<br />

DNA

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