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ex vivo
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ex vivo
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Nature Methods , 227
230, 2007
PNAS , 1347513480, 2009
PLoS ONE , e21531, 2011
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Tight junctions prevent the leakage of solutes through the paracellular
pathway of epithelial cells and are indispensable in establishing the
various compositionally distinct fluid compartments within the body
of multicellular organisms.
Based on morphological observations, two models about the tight
junction have been proposed. The protein model proposes that a
linear array of membrane protein particles polymerize to form a tight
junction strand and that a network of these strands adhere with paired
strands from apposing cells to obliterate the intercellular space. On
the other hand, the lipid model, which originates from the observation
of exoplasmic lipid leaflet fusion, proposes that the fusion of these
lipid leaflets is associated with a transformation of the lamellar phase
of the lipid bilayers into a non-lamellar, inverted micelle structure at
tight junctions.
Much has been learned about the membrane proteins at tight junctions
over the last decade. However, how these membrane proteins function
as the barriers in the paracellular space remains to be elucidated.
Here, I will present recent progress toward a better understanding of
supra-molecular complex at tight junctions.