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P
FABP
FABP7
Kupffer KC
KC FABP7
FABP7 KC
FABP7 clodoronateliposome clo
lipo KC FABP7
clolipo 7 F4/80+KC
FABP7
clolipo 7 F4/80+KC 78% FABP7+
FABP7 J774 FABP7/J774LPS
TNFα TNFα
IκB
FABP7 FABP7
KC
P
1,3 Randeep Rakwal 1 1 1 1
1 1 2 3 1
1
2 3 NPO
1/3
1.2% 5 / 1.2%NP 4
6 LPS 10 mg/
kg mRNA DyeSwap
DNA 1.2%NP 5000
1000
P
Functional Analysis of Nucleoprotein diet from salmon testis, Shirako
via Intestinal IgA secretion and TLR stimulation
1 Randeep Rakwal 1 1 1 1
1 1 2 3 1 1
1
2 3 NPO
Salmon testis, Shirako contains in plenty nucleic acids and protamin protein,
termed as Nucleoprotein NP. However, we know little about their functions in
the human body, and molecular evidence for these potential beneficial effects.
Therefore, the aim of this study was to elucidate the effects of dietary NP and
know molecular evidence for these potential beneficial effects.
First, we analyzed the innate immunity response in mice by measuring IgA
secretion in feces. To do so, mice were fed high or low level NP diets for 4 weeks.
Fecal IgA level was measured with an ELISA kit. Our results revealed that the
fecal level of IgA was little higher in the highNP diets group than in the lowNP
diets group.
Next, we examined the human TLR9 stimulation and cytokine production
by effect of NP. We added NP into HEK293 cells overexpressed human TLR
9 to measure NFκB transcriptional activity and, PMA induced U937 cells to
measure several cytokine expressions. The results show that NP affects on TLR9
stimulation and some cytokine expressions.
Here, we show some evidences and hypothesize that NP diet might affect on some
cytokine expressions to increase mucosal immunity.
P
The effects of adjuvants on autoimmune responses against testicular
antigens in mice
Musha Muhetaerjiang, Shuichi Hirai, Munekazu Naito, Hayato Terayama,
Qu Ning, Masahiro Itoh
A subcutaneous injection with testicular homogenate TH can easily induce
systemic immune responses against the autoantigens of germ cells. Experimental
autoimmune orchitis EAO is pathologically characterized by lymphocytic
inflammation accompanied by the spermatogenic disturbance. Classically, the
murine EAO is induced by immunization with TH + Complete Freund’s Adjuvant
+ Bordetella Pertussigens, and it has been considered that these adjuvants is
needed to enhance the immune responses. However, there remains a possibility
that adjuvants affect autoimmune responses against the autoantigens without
TH. In the present study, we examined this possibility using real time RTPCR,
Western Blotting and immunohistochemical staining. The results demonstrated
that testicular histopathological state and expressions of intratesticular cytokines
were normal in mice injected with adjuvants alone. However, various kinds of
serum autoantibodies with not only germ cells but also Sertoli cells were detected
in the adjuvant injected mice. These results indicate that adjuvants alone can
evoke testicular autoimmune reactions against various autoantigens irrespective of
no use of TH.
P
2
P
Germ cell death in testicular autoimmunity
Kuerban Maimaiti, Shuichi Hirai, Hayato Terayama, Qu Ning, Yuki Ogawa,
Musha Muhetaerjiang, Munekazu Naito, Masahiro Itoh
Experimental autoimmune orchitis EAO is characterized by T celldependent
lymphocytic inflammation and damage to seminiferous tubules, causing death of
testicular germ cell TGC. The aim of the present study is to investigate the role
of apoptosis systems in the TGC death in EAO in mice, using real time RTPCR
and immunostaining. The results showed that the many Tdt mediated dUTP nick
end labeling positive TGC were found at the active EAO stage and persistently
observed in the seminiferous epithelium until the postactive EAO stage. Intra
testicular mRNAs expression of both Fas and Bax increased at the active EAO
stage and dramatically decreased at the postactive EAO stage. In contrast, intra
testicular the mRNAs expression of both FasL and Bcl2 did not show significant
changes at the active EAO stage but extremely increased at the postactive EAO
stage. Immunohistochemically, some Fas or Baxpositive TGC were detected at
the active EAO stage and hardly found at the postactive EAO stage. In contrast,
some FasL or Bcl2 positive TGC were found at the active EAO stage but many
of them were observed at the postactive EAO stage.