More documents

Recommendations

Info

166 117 P FABP FABP7 Kupffer KC KC FABP7 FABP7 KC FABP7 clodoronateliposome clo lipo KC FABP7 clolipo 7 F4/80+KC FABP7 clolipo 7 F4/80+KC 78% FABP7+ FABP7 J774 FABP7/J774LPS TNFα TNFα IκB FABP7 FABP7 KC P 1,3 Randeep Rakwal 1 1 1 1 1 1 2 3 1 1 2 3 NPO 1/3 1.2% 5 / 1.2%NP 4 6 LPS 10 mg/ kg mRNA DyeSwap DNA 1.2%NP 5000 1000 P Functional Analysis of Nucleoprotein diet from salmon testis, Shirako via Intestinal IgA secretion and TLR stimulation 1 Randeep Rakwal 1 1 1 1 1 1 2 3 1 1 1 2 3 NPO Salmon testis, Shirako contains in plenty nucleic acids and protamin protein, termed as Nucleoprotein NP. However, we know little about their functions in the human body, and molecular evidence for these potential beneficial effects. Therefore, the aim of this study was to elucidate the effects of dietary NP and know molecular evidence for these potential beneficial effects. First, we analyzed the innate immunity response in mice by measuring IgA secretion in feces. To do so, mice were fed high or low level NP diets for 4 weeks. Fecal IgA level was measured with an ELISA kit. Our results revealed that the fecal level of IgA was little higher in the highNP diets group than in the lowNP diets group. Next, we examined the human TLR9 stimulation and cytokine production by effect of NP. We added NP into HEK293 cells overexpressed human TLR 9 to measure NFκB transcriptional activity and, PMA induced U937 cells to measure several cytokine expressions. The results show that NP affects on TLR9 stimulation and some cytokine expressions. Here, we show some evidences and hypothesize that NP diet might affect on some cytokine expressions to increase mucosal immunity. P The effects of adjuvants on autoimmune responses against testicular antigens in mice Musha Muhetaerjiang, Shuichi Hirai, Munekazu Naito, Hayato Terayama, Qu Ning, Masahiro Itoh A subcutaneous injection with testicular homogenate TH can easily induce systemic immune responses against the autoantigens of germ cells. Experimental autoimmune orchitis EAO is pathologically characterized by lymphocytic inflammation accompanied by the spermatogenic disturbance. Classically, the murine EAO is induced by immunization with TH + Complete Freund’s Adjuvant + Bordetella Pertussigens, and it has been considered that these adjuvants is needed to enhance the immune responses. However, there remains a possibility that adjuvants affect autoimmune responses against the autoantigens without TH. In the present study, we examined this possibility using real time RTPCR, Western Blotting and immunohistochemical staining. The results demonstrated that testicular histopathological state and expressions of intratesticular cytokines were normal in mice injected with adjuvants alone. However, various kinds of serum autoantibodies with not only germ cells but also Sertoli cells were detected in the adjuvant injected mice. These results indicate that adjuvants alone can evoke testicular autoimmune reactions against various autoantigens irrespective of no use of TH. P 2 P Germ cell death in testicular autoimmunity Kuerban Maimaiti, Shuichi Hirai, Hayato Terayama, Qu Ning, Yuki Ogawa, Musha Muhetaerjiang, Munekazu Naito, Masahiro Itoh Experimental autoimmune orchitis EAO is characterized by T celldependent lymphocytic inflammation and damage to seminiferous tubules, causing death of testicular germ cell TGC. The aim of the present study is to investigate the role of apoptosis systems in the TGC death in EAO in mice, using real time RTPCR and immunostaining. The results showed that the many Tdt mediated dUTP nick end labeling positive TGC were found at the active EAO stage and persistently observed in the seminiferous epithelium until the postactive EAO stage. Intra testicular mRNAs expression of both Fas and Bax increased at the active EAO stage and dramatically decreased at the postactive EAO stage. In contrast, intra testicular the mRNAs expression of both FasL and Bcl2 did not show significant changes at the active EAO stage but extremely increased at the postactive EAO stage. Immunohistochemically, some Fas or Baxpositive TGC were detected at the active EAO stage and hardly found at the postactive EAO stage. In contrast, some FasL or Bcl2 positive TGC were found at the active EAO stage but many of them were observed at the postactive EAO stage.
117 167 P 1 2 1 3 1 1 2 3 A,B,C D B PNAS, Yuan et al. 2007 152 CD68S100 Fascin P 1 conduit ICR 2000 kDa 10 kDa 310 4 PFA 3 530 OCT 10 μm Tris ER TR7 TypeIV 5 nm 54 nm P trafficking secondary lymphoid organ, SLO traffickingT SLO T DC DC T T trafficking molecule DC trafficking DC DC T DC trafficking SLO P Alterations of synaptic transcytosis induced by ethanol exposure: apocrinelike structure in the rat Tomiko Yakura 1 , Takanori Miki 1 , JunQian Liu 1 , Kenichi Ohta 1 , Katsuhiko Warita 1 , Yoshiki Matsumoto 2 , Shingo Suzuki 1 , Motoki Tamai 1 , Yoshiki Takeuchi 1 1 Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 2 Laboratory of Animal Science, Faculty of Agriculture, Kagawa University Effects of alcohol exposure on synaptic structure were investigated in the NST in the rat. Ethanolfed animals were allowed free access to liquid diet containing ethanol for 3 weeks. A few terminals were characterized by deep indentation of axodendritic membranes into the postsynaptic neurons which was similar to the apocrine structure. Other animals of ethanol exposure received injection of WGAHRP into the vagus nerve and were prepared for electron microscopy. HRPreaction product RP was recognized easily as electron dense lysosomal substance when lead citrate staining was omitted. The terminals containing HRP RP also revealed quite similar structure to indentation of axodendritic membranes described above. The results are considered to confirm that terminals forming “apocrine-like structure” are originated from afferent fibers of the vagus nerve. The present study raised the possibility that remarkable alteration of the synaptic structure induced by ethanol exposure leads neuronal transcytosis of materials including proteins which is absolutely different from vesicular exocytosis involved in chemical synaptic transmission. P LAMP Lysosomeassociated membrane protein2 LAMP2 LAMP2 X LAMP2 12 LAMP2 LAMP1 D A GM130 LC3 LAMP2 LAMP2 P Synergistic interaction between Golgi outposts and RNA granules in postlocal translational secretory pathway Souichi Oe, Yasuko Noda Jichi Med. Univ. Tochigi, Japan In the central nervous system, neurons have Golgi outposts in dendrites and it has been shown that its subcellular distribution and morphology are related with various aspects of cellular event. In this study, we analyzed the subcellular localization of Golgi outposts under the various stimulations in cultured hippocampal neurons. In normal conditions, Golgi outposts localized in basal dendrites and contained some Golgi matrix proteins including GM130, GRASP65 and BicaudalD2. When the neurons were cultured with several stimulations, the distribution of Golgi outposts were enhanced towards the distal dendrites. This observation was similar to the dynamics of RNA granules, and in fact, Golgi outposts colocalized with CPEB which is the translational repressor and the component of the RNA granules. Furthermore, we constructed the GFP based reporter plasmid that has the signal sequence and the dendritic localization signal of CaMKII mRNA and observed the localization in dendrites with or without the stimulations. These results suggest that Golgi outposts act synergistically with RNA granules and contribute to the postlocal translational secretory pathway in dendrites.
Page 5 and 6:
2 4 5 6 8 9 10 12 12 13 1
Page 7 and 8:
117 3 1 1 1980 4 2002 200
Page 9 and 10:
117 5 100 http://yamanashiko
Page 11 and 12:
117 7
Page 13 and 14:
117 9 1117TEL: 0552541000 URL:
Page 15 and 16:
117 11 Y 1 Y12 3 26 8001930
Page 17 and 18:
117 13 8 4 PC PC PC
Page 19 and 20:
117 15 24 3 28 9151530 3 28
Page 21 and 22:
117 17
Page 23 and 24:
117 19 2 1P0
Page 25 and 26:
117 21 4008510 4437FAX 055
Page 27 and 28:
117 23 TEL0196515110 5880FAX01990
Page 29:
117
Page 32 and 33:
26 117 ::B M 15 30
Page 34 and 35:
28 S S S S 117 ex vivo
Page 36 and 37:
30 S 117 Coordinated branching mo
Page 38 and 39:
32 117 ::A M M S. 2
Page 40 and 41:
34 117 ::A M M S. S
Page 42 and 43:
36 117 S kisspeptin S
Page 44 and 45:
38 117 S S ::B M M S.
Page 46 and 47:
40 117 E L C LC ::OEPM I
Page 48 and 49:
42 117 E L C LC ::OEAM III
Page 50 and 51:
44 117 2OHAMIII2 2OHAMIII3 1
Page 52 and 53:
46 117 F L C LC ::OFPM VI 3
Page 54 and 55:
48 117 :: I 1P001 S100A
Page 56 and 57:
50 117 1P073 Makoto Naganuma Acti
Page 58 and 59:
52 117 2P018 Conserved properti
Page 60 and 61:
54 117 2P090 2P091 2P092
Page 62 and 63:
56 117 3P027 3P028 ABC 3P0
Page 64 and 65:
58 117 :: 3P102 3P103
Page 67 and 68:
117 59 100 μm 20 nm 1 μ
Page 69 and 70:
117 61 12 345
Page 71 and 72:
117 63 SM G
Page 73 and 74:
117 65 S Physiological roles of ce
Page 75 and 76:
117 67 S 1,2 1 1 2 JST S
Page 77 and 78:
117 69 S The role of autophagyrela
Page 79 and 80:
117 71 S - ATP ionom
Page 81 and 82:
117 73 S 3D 2 3D 3
Page 83 and 84:
117 75 S 1 2 1 1 1
Page 85 and 86:
117 77 S GSK3β gl
Page 87 and 88:
117 79 S niche DNA endos
Page 89 and 90:
117 81 S GABA GABA Cl KCC2C
Page 91 and 92:
117 83 S By means of retrograd
Page 93 and 94:
117 85 S S
Page 95 and 96:
117 87 S 1,2,3 1 2 CREST, JS
Page 97 and 98:
117 89 S C 1 1 2 1 2 1
Page 99 and 100:
117 91 S Focused Ion BeamScanning
Page 101 and 102:
117 93 S 2
Page 103 and 104:
117 95 S S
Page 105 and 106:
117 97 W WG 1,2 1 2 WG
Page 107 and 108:
117 99 OEPMI Singleneuron tracing
Page 109 and 110:
117 101 OFPMII 1 2 3 2
Page 111 and 112:
117 103 OHPM Two immunogenic passe
Page 113 and 114:
117 105 OEAMIII PHAL
Page 115 and 116:
117 107 OFAMIV 1 1 1 2
Page 117 and 118:
117 109 OGAMII BrdU Labelr
Page 119 and 120:
117 111 OHAMIII 1 2 2 2
Page 121 and 122:
117 113 ODPMI Large Maf Large
Page 123 and 124: 117 115 OEPMVII 1 1 2 2
Page 125 and 126: 117 117 OFPMVIII α
Page 127 and 128: 117 119 OHPMIV 1
Page 129 and 130: 117 121 OIPMIV 1 2 1 1
Page 131 and 132: 117 123 P SA 1 2 1 2 Glial
Page 133 and 134: 117 125 P γ 4,52 P
Page 135 and 136: 117 127 P cFos 1 2 1 1
Page 137 and 138: 117 129 P Identification of CSPG c
Page 139 and 140: 117 131 P NOS 1 2 4 4
Page 141 and 142: 117 133 P DPEP 1 2 2 1
Page 143 and 144: 117 135 P Activities of human uppe
Page 145 and 146: 117 137 P 2010 77 2
Page 147 and 148: 117 139 P Nacholapithecus kerioi
Page 149 and 150: 117 141 P kirrel 1 1 1
Page 151 and 152: 117 143 P prechordal chond
Page 153 and 154: 117 145 P FIB/SEM 1,3 1 2
Page 155 and 156: 117 147 P VIP 12 VIP
Page 157 and 158: 117 149 P Motor protein KIFA is es
Page 159 and 160: 117 151 P parvic
Page 161 and 162: 117 153 P SEM 75 mM KC
Page 163 and 164: 117 155 P mAtgAAcGFP mDFCPmCherry
Page 165 and 166: 117 157 P αMangostin 1 2
Page 167 and 168: 117 159 P 1 1 1 1 2 1
Page 169 and 170: 117 161 P planar cell polarity
Page 171 and 172: 117 163 P The metabolism of choles
Page 173: 117 165 P 1 1 2 2 1 1
Page 177 and 178: 117 169 P Agerelated changes in es
Page 179 and 180: 117 171 P PACAP 1,2 1,3 Ran
Page 181 and 182: 117 173 P Vc Vc M
Page 183 and 184: 117 175 P C β ISL1 NKX2.2
Page 185 and 186: 117 177 P 1 2 3 4,5 4
Page 187 and 188: 117 179 P TIRF ECM ECM 1
Page 189 and 190: 117 181 P 1 1 1 1 1
Page 191 and 192: 117 183 P 1 2 1 1 1 1
Page 193 and 194: 117 185 P NMJ NMJ d
Page 195 and 196: 117 187 P 2023 66 R
Page 197 and 198: 117 189 P 1 1 1 1 1
Page 199: 117
Page 202 and 203: 192 117 Marson, Lesley Masahiro,
Page 204 and 205: 194 117 1OFPMI2 1OFPM
Page 206 and 207: 196 117 3P042 2OFAMV2 3OEPM
Page 208 and 209: 198 117 1P015 2P020
Page 210 and 211: 200 117 2OHAMI1 2OHAMIII3 1P0
Page 212 and 213: 202 117 S36 1P097 S95 S52
Page 214 and 215: 204 117 3OEPMVII
Page 216 and 217: 206 117 1P022 2P130
Page 219: 117 209 117 2012 2 22
Page 227 and 228:
3D Helios NanoLab TM •3D •
show all

ç¬¬117åæ¥æ¬è§£åå­¦ä¼ç·ä¼ã»å ¨å½å­¦è¡éä¼ è¬æ¼ãã­ã°ã©ã ã»æé²é PDF ...

Create successful ePaper yourself

Delete template?

Save as template?

ç¬¬117åæ¥æ¬è§£åå¦ä¼ç·ä¼ã»å¨å½å¦è¡éä¼ è¬æ¼ããã°ã©ã ã»æé²é PDF ...