第117å›žæ—¥æœ¬è§£å‰–å­¦ä¼šç·ä¼šãƒ»å ¨å›½å­¦è¡“é›†ä¼š 講演プログラム・抄録集 PDF ...

117 163
P
The metabolism of cholesterol after bile duct degeneration in lamprey
Mayako Morii 1 , Yoshihiro Mezaki 2 , Noriko Yamaguchi 2 , Kiwamu Yoshikawa 2 ,
Mitsutaka Miura 2 , Katsuyuki Imai 2 , Taku Hebiguchi 1 , Ryo Watanabe 1 ,
Hiroaki Yoshino 1 , Haruki Senoo 2
1
Department of Pediatric Surgery, Akita University Graduate School of Medicine,
2
Department of Cell Biology and Morphology, Akita University Graduate School
of Medicine
Lampreys are unique vertebrates in that they lose biliary trees entirely during
the metamorphosis. Despite the biliary atresia, lampreys do not develop neither
biliary cirrhosis nor liver dysfunction. In other vertebrates, obstruction of bile
ducts results in fatality because of the cytotoxicity of bile salts. It means that
lampreys have evolved to use another metabolic pathway of bile juice in order
to evade the cholestasis. However, molecular mechanism of this pathway is still
unknown. The aim of this study is to investigate the way how the lamprey avoid
the pathological consequence of cholestasis. We have cloned the cDNA sequences
of two kinds of P450, which show high degree of homology with mammalian
CYP7A1 and CYP27A1, respectively. We are now evaluating the expression
levels of the mRNA for these genes in liver, gonad, gill, heart and intestine along
with the temporal expression profiles from larva to adult. The present study
suggests how and where bile acids are detoxified in adult lampreys. Understanding
how lampreys avoid cholestasis could be valuable for progress in the treatment of
human obstructive cholangiopathy.
P
NAFLD
1 1 1 2 1
1
2
NAFLD NAFLD
NASH NAFLD
NASH
9 C57BL/6 MCD
30 M R MCD 8 16
30 2 AST, ALT
M 2 AST, ALT
NAFLD
10
M 16 CD68
CD68
R 3
R 16
MCD 16 CD68
30
CD68
P
Deltalike
Deltalike3 DLL3
DLL3
Huh2 DLL3
AnexinV TUNEL singlestrand DNA
DLL3 Huh2
DLL3 binding
assay DLL3 Small G Cdc42
CRIB
DLL3 HuH2 Cdc42
PAK1 MAP kinase
DLL3
DLL3 Cdc42 PAK1 MAP kinase
P
1 2 2 2 1
1
2
CLD
ICR 85O 2
RA21Air 14
RA 7 RA14 Air14d
14 O 2 14dRA 21 Air21dO 2 Air21d
4%PFA
O 2 14d
O 2
Air21d
CLD
P
CEACAM
1 Nabil Eid 1 2 1 1
1
2
prox1
Carcinoembryonic cell adhesion molecule1 CEACAM1
CEACAM1
VEGFC
BJMC338 VEGFC
BJMC3879
2 CEACAM1,
VEGFR3VEGFR3
phosphotyrosine
VEGFA
BJMC3879
CEACAM1 BJMC3879
CEACAM1 BJMC338
BJMC3879
BJMC3879 VEGFR3
BJMC3879 CEACAM1
VEGFC
P
The role of peritoneal lymphatic system on the human peritoneal
dissemination
Masahiro Miura 1 , Yutaka Yonemura 2 , Yoshio Endou 3
1
Department of Human Anatomy, Faculty of Medicine, Oita University, 2 NPO
organization to support peritoneal dissemination treatment, 3 Department of
Molecular Targeting Therapy, Kanazawa University
The role of lymphatic microenvironment on cancer metastasis was studied.
VEGFCgene transfectants of the gastric cancer cell lines were inoculated into
the peritoneal cavity of nude mice. The tissue samples from human disseminated
peritoneum were stained with 5,NaseALPase double staining and aniti
VEGFR3 immunostaining. Mouse VEGFR3 gene expression was induced after
inoculation. In the early peritoneal dissemination PD stage, newly lymphatic
networks LN were detected in the periphery of the primary tumor. The main
source of lymphatic dissemination may originate from the peritumoral LN. In the
PD, three patterns of tumor lymphangiogenesis, namely centrifugal, centripetaland
combination growth patterns were found. Free cancer cells invaded the numerous
blind endings that came from extended subperitoneal LN. In the late PD stage,
the number of LN decreased due to the destruction of cancer cells. In SEM
observation, Macula cribriformis were seen in the diaphragmatic subperitoneal
tissue layer and Morrison pouch. Lymphangioginesis including newly formed LN,
induced by VEGFC is closely associated in formation of PD and lymph node
metastasis mainly via the prelymphatic channels.