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3.4 Capítulo 4 Effect of different periods of preconditioning with saliva on adhesion of C. albicans to a denture base resin by crystal violet staining and XTT assay Short title: Effect of saliva on adhesion of Candida Camila Andrade Zamperini 1, Patrícia Cristiane dos Santos Schiavinato 1, Ana Lucia Machado 1*, Eunice Teresinha Giampaolo 1, Ana Claudia Pavarina 1, Carlos Eduardo Vergani 1 1 Araraquara Dental School, UNESP - Univ Estadual Paulista, Department of Dental Materials and Prosthodontics, Araraquara, São Paulo, Brazil. *Corresponding author: Profa. Dra. Ana Lucia Machado Araraquara Dental School, UNESP – Univ Estadual Paulista, Department of Dental Materials and Prosthodontics, Araraquara, São Paulo, Rua Humaitá nº 1680, CEP 14.801-903, Brazil. Tel: 55-16-33016410 Fax: 55-16-33016406. Email: cucci@foar.unesp.br
114 Abstract The role of saliva on Candida adhesion to biomaterials is not clearly defined. Aim: this study investigated whether different periods of preconditioning with saliva would influence the adhesion of Candida albicans to a denture base resin. Methods: samples (n=90) with smooth surfaces were made and then divided into five groups: 1 control - without saliva; 4 experimental groups – conditioned in saliva for periods of: 30, 60, 180 or 720 minutes. Candida adhesion was evaluated by crystal violet staining and XTT assay. Results: the one-way analyses of variance revealed that there were no significant differences among the mean numbers of adherent cells or among the mean absorbances for all groups. No significant correlation was found between the two methods used for assessing C. albicans adhesion. Conclusions: the different periods of preconditioning with saliva had no significant influence on the adhesion of Candida albicans to the denture base acrylic resin. Key words: Acrylic Resins; Biofilms; Adhesion; Candida albicans; Saliva.
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UNIVERSIDADE ESTADUAL PAULISTA “J
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iii Dados Curriculares Camila Andra
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v exemplos, de quem eu me orgulho m
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vii Agradecimentos Especiais Muito
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ix mais novo”! Se eu pudesse, tor
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xi Aos meus amigos de turma do curs
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xiii À Capes (Coordenação de Ape
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Sumário Resumo....................
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ii alterações significantes nos v
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ii adherence of Candida albicans, e
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21 como: não eliminação do micro
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23 mais hidrofóbica a superfície,
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25 2008), resultando em uma superf
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3 Capítulos 3.1 Capítulo 1 Adhere
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31 Introduction The inability of cu
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33 negatively charged surfaces 22 .
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35 Plasma Treatments After roughnes
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37 measurements were performed in a
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39 was prepared in acetone at 0.4 m
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41 forces, the development of the m
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43 adherence observed in the presen
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45 effect 27,30,42-44 or decreased
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47 3 Yildirim MS, Hasanreisoglu U,
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49 of Thai silk treated by SF 6 pla
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51 33 Taylor R, Maryan C, Verran J.
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53 47 Waters MGJ, Williams DW, Jagg
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55 Table 2 - Means and standard dev
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Figure 2 57
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3.2 Capítulo 2 Evaluation of funga
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61 adhering to denture surfaces [1]
Page 64 and 65: 63 mimicking the tissue-fitting sur
Page 66 and 67: 65 spectroscopy analysis (XPS), car
Page 68 and 69: 67 suspension was added to each wel
Page 70 and 71: 69 Initial adherence of Candida alb
Page 72 and 73: 71 increase probably occurred by me
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Page 76 and 77: 75 3) For all groups evaluated, no
Page 78 and 79: 77 12. Pereira-Cenci T, Cury AADB,
Page 80 and 81: 79 25. Ramage G, Tomsett K, Wickes
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Page 84 and 85: 83 Tables Table 1 - Means and stand
Page 86 and 87: 85 Figures Figure 1
Page 88 and 89: 87 Figure Legends Figure 1 - XPS an
Page 90 and 91: 89 Abstract Candida adhesion to pol
Page 92 and 93: 91 to acrylic surfaces of Candida g
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Page 96 and 97: 95 average was calculated. Specimen
Page 98 and 99: 97 Statistical Analysis Differences
Page 100 and 101: 99 the C. glabrata adhesion. The me
Page 102 and 103: 101 One other important observation
Page 104 and 105: 103 6. Minagi S, Miyake Y, Inagaki
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Page 108 and 109: 107 33. Polukoshko KM, Brudvik JS,
Page 110 and 111: 109 Tables Table 1 - Means and stan
Page 112 and 113: 111 5.0 Log (cel/mm2) 4.0 3.0 2.0 *
Page 116 and 117: 115 Introduction The presence of Ca
Page 118 and 119: 117 Italy) measuring 13.8 X 2 mm we
Page 120 and 121: 119 4 - 6 ºC during the experiment
Page 122 and 123: 121 Candida adhesion to dentures, a
Page 124 and 125: 123 absorbance values obtained afte
Page 126 and 127: 125 1. Silva WJ, Seneviratne J, Par
Page 128 and 129: 127 14. Karaagaclioglu L, Can G, Yi
Page 130 and 131: 129 28. Hahnel S, Rosentritt M, Han
Page 132 and 133: 131 Tables Table 1 Table 1 - Median
Page 134 and 135: 3.5 Capítulo 5 The effect of human
Page 136 and 137: 135 Introduction An important step
Page 138 and 139: 137 albicans adhesion to a denture
Page 140 and 141: 139 For group G 3 , whole human uns
Page 142 and 143: 141 each specimen, using a light mi
Page 144 and 145: 143 was collected from one donor or
Page 146 and 147: 145 In conclusion, this study focus
Page 148 and 149: 147 11. Edgerton M, Scannapieco FA,
Page 150 and 151: 149 24. Thein ZM, Samaranayake YH,
Page 152 and 153: 151 37. Zamperini CA, Machado AL, V
Page 154 and 155: 153 Figures Figure 1 *
Page 156: 155 Figure Legends Figure 1. Mean a
Page 159 and 160: 158 significantemente menores quand
Page 161 and 162: 160 diminuindo os valores de ângul
Page 163 and 164: 162 Nos estudos apresentados nos ca
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164 pela coloração cristal violet
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166 Diferentemente dos resultados o
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5 Conclusão Dentro das limitaçõe
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172 6. Bürgers R, Hahnel S, Reiche
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174 22. Hodak SK, Supasai T, Paosaw
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176 40. Nikawa H, Chen J, Hamada T,
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178 56. Pusateri CR, Monaco EA, Edg
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180 73. Tari BF, Nalbant D, Al DF,
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7 Anexos Anexo 1
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Anexo 3
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Anexo 5
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Anexo 7
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8 Apêndice Apêndice 1 Os resultad
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194 Tabela 3A - Grupo submetido ao
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Apêndice 2 Os resultados obtidos d
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224 Os resultados obtidos durante a
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