universidade de são paulo - Faculdade de Odontologia - Unesp

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Statistical Analysis
Differences in the adherent yeast cells (crystal violet staining assay)
among the experimental conditions were evaluated by two-way analysis of
variance followed by Tukey’s test. Data of yeast counts (cells mm -2 ) were
transformed by log. For each group, statistical analysis was performed using the
paired Student's t test to evaluate the effect of the time of measurement on the
contact angle. One-way analysis of variance, followed by Tukey’s test, was used
to determine differences in the contact angle among groups after 48 hours of
immersion in water. Comparison of the roughness values among the groups was
performed by the non-parametric Kruskal-Wallis test. A significance level of 0.05
was used for all statistical tests.
Results
Candida glabrata adherence as determined by crystal violet staining assay
is shown in Figure 1. In the absence of saliva, group Ar/50W showed significantly
lower Candida glabrata adherence than the control group (P.05), in the presence of saliva. Figure 1 also
shows that, within each group, there was no significant difference between
absence and presence of saliva. All negative controls exhibited no metabolic
activity (data not shown). Table 1 shows the means and standard deviations of
contact angle. There was significant change in the mean contact angle after 48 h of
immersion in water, for all groups evaluated (P