universidade de são paulo - Faculdade de Odontologia - Unesp

ii
adherence of Candida albicans, evaluated by crystal violet staining, was observed
between the plasma treated and control groups, irrespective of the presence or
absence of saliva, and surface roughness. The number of adhered Candida
glabrata, evaluated by counting after crystal violet staining, was significantly
lower in Ar/50W group than the control group, in the absence of saliva. However,
after preconditioning with saliva, Candida glabrata adherence in experimental
and control groups did not differ significantly. The different periods of
preconditioning with saliva had no significant influence in the Candida albicans
adhesion, but the centrifugation parameters (speed and time) and number of
donors of saliva influenced the results of Candida albicans adhesion to the
denture base acrylic resin. No significant correlation was found between the two
methods used for assessing Candida albicans adhesion, crystal violet staining and
XTT reduction method. Thus, the results demonstrated that ArO 2 /70W and
ArSF 6 /70W plasma treatments showed promising potential for reducing Candida
albicans adhesion, while the Ar/50W plasma treatment showed promising
potential for reducing Candida glabrata adhesion to denture base resins.
Moreover, the saliva pellicle, depending of experimental conditions, may increase
the Candida albicans adhesion, but it not significantly influences the Candida
glabrata adhesion. The diverse methodological procedures regarding to
preconditioning with saliva alter the results of Candida albicans adhesion.
Keywords: Cell adherence; biofilms; Candida albicans; Candida glabrata; acrylic
resins;
saliva.