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33 negatively charged surfaces 22 . These results suggest that the surface of materials used in removable complete and partial denture could be modified by plasma treatment, preventing such surfaces to act as infection reservoirs. However, information on the adhesion of Candida albicans to glow-discharge modified acrylic denture base polymers are scarce and only oxygen plasma treatment was evaluated 3 . Moreover, surface modification of denture base resins by fluorine plasma treatment still remains to be investigated. The main purpose of the present in vitro study was to investigate the potential of different plasma treatments to modify a denture base acrylic resin to reduce the Candida albicans adhesion. The effect of substrate surface roughness and saliva coating was also evaluated. Materials and Methods Preparation of Acrylic Resin Specimens The specimens (n=180) were fabricated from an acrylic resin denture base material (Vipi Wave - VIPI Indústria e Comércio Exportação e Importação de Produtos Odontológicos Ltda Pirassununga, SP, Brazil) using a conventional flasking and pressure-pack technique. Initially, a metal mold was used to make disk-shaped silicone patterns Zetaplus/Indurent - Zhermack, Badia Polesine, Rovigo, Italy) measuring 13.8 X 2 mm. Half of the silicone patterns were invested in the flaks directly in dental stone, while the other half of the patterns were sandwiched between two glass slides before investing. These two types of investing techniques were used to obtain rough and smooth specimens, thus mimicking the tissue-fitting surface and the outer surface of dentures,
34 respectively. The flasks were separated, the silicone patterns were removed, and the stone surfaces were painted with a separating medium (Vipi Film - VIPI Indústria e Comércio Exportação e Importação de Produtos Odontológicos Ltda Pirassununga, SP, Brazil). For each specimen, 1 g of powder and 0.47 mL of monomer liquid were mixed and processed according to the manufacturer’s instructions. The mixture was packed into the molds, a trial pack was completed, and excess material was removed. A final pack was performed and held for 15 minutes. The denture base acrylic resin was processed in a 500 W domestic microwave oven (Brastemp – Brastemp da Amazonia SA, Manaus, AM, Brazil) for 20 minutes at 20% power, followed by 5 minutes at 90% power. The flasks were allowed to bench cool at room temperature, the specimens were deflasked, and excess flash was aseptically removed with a sterile bur (Maxi-Cut; Lesfils de August Malleifer SA, Ballaigues, Switzerland). Surface Roughness Measurements The surface roughness of all specimens was measured with a profilometer (Mitutoyo SJ 400 – Mitutoyo Corporation - Japan). Three measurements were made for each specimen and the average reading was designated as the Ra (μm) value of that specimen. Resolution was 0.01 μm, interval (cutoff length) was 0.8 mm, transverse length was 2.4 mm, the stylus speed was 0.5 mm/s, and the diamond stylus tip radius was 5 μm. All measurements were recorded by one operator.
Page 1 and 2: UNIVERSIDADE ESTADUAL PAULISTA “J
Page 3 and 4: iii Dados Curriculares Camila Andra
Page 5 and 6: v exemplos, de quem eu me orgulho m
Page 7 and 8: vii Agradecimentos Especiais Muito
Page 9 and 10: ix mais novo”! Se eu pudesse, tor
Page 11 and 12: xi Aos meus amigos de turma do curs
Page 13 and 14: xiii À Capes (Coordenação de Ape
Page 15 and 16: Sumário Resumo....................
Page 17 and 18: ii alterações significantes nos v
Page 19: ii adherence of Candida albicans, e
Page 22 and 23: 21 como: não eliminação do micro
Page 24 and 25: 23 mais hidrofóbica a superfície,
Page 26: 25 2008), resultando em uma superf
Page 30 and 31: 3 Capítulos 3.1 Capítulo 1 Adhere
Page 32 and 33: 31 Introduction The inability of cu
Page 36 and 37: 35 Plasma Treatments After roughnes
Page 38 and 39: 37 measurements were performed in a
Page 40 and 41: 39 was prepared in acetone at 0.4 m
Page 42 and 43: 41 forces, the development of the m
Page 44 and 45: 43 adherence observed in the presen
Page 46 and 47: 45 effect 27,30,42-44 or decreased
Page 48 and 49: 47 3 Yildirim MS, Hasanreisoglu U,
Page 50 and 51: 49 of Thai silk treated by SF 6 pla
Page 52 and 53: 51 33 Taylor R, Maryan C, Verran J.
Page 54 and 55: 53 47 Waters MGJ, Williams DW, Jagg
Page 56 and 57: 55 Table 2 - Means and standard dev
Page 58 and 59: Figure 2 57
Page 60 and 61: 3.2 Capítulo 2 Evaluation of funga
Page 62 and 63: 61 adhering to denture surfaces [1]
Page 64 and 65: 63 mimicking the tissue-fitting sur
Page 66 and 67: 65 spectroscopy analysis (XPS), car
Page 68 and 69: 67 suspension was added to each wel
Page 70 and 71: 69 Initial adherence of Candida alb
Page 72 and 73: 71 increase probably occurred by me
Page 74 and 75: 73 to the adsorption of salivary pr
Page 76 and 77: 75 3) For all groups evaluated, no
Page 78 and 79: 77 12. Pereira-Cenci T, Cury AADB,
Page 80 and 81: 79 25. Ramage G, Tomsett K, Wickes
Page 82 and 83: 81 38. Nikawa H, Hayashi S, Nikawa
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83 Tables Table 1 - Means and stand
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85 Figures Figure 1
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87 Figure Legends Figure 1 - XPS an
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89 Abstract Candida adhesion to pol
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91 to acrylic surfaces of Candida g
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93 minutes. The denture base acryli
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95 average was calculated. Specimen
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97 Statistical Analysis Differences
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99 the C. glabrata adhesion. The me
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101 One other important observation
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103 6. Minagi S, Miyake Y, Inagaki
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105 19. Nikawa H, Hayashi S, Nikawa
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107 33. Polukoshko KM, Brudvik JS,
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109 Tables Table 1 - Means and stan
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111 5.0 Log (cel/mm2) 4.0 3.0 2.0 *
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3.4 Capítulo 4 Effect of different
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115 Introduction The presence of Ca
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117 Italy) measuring 13.8 X 2 mm we
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119 4 - 6 ºC during the experiment
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121 Candida adhesion to dentures, a
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123 absorbance values obtained afte
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125 1. Silva WJ, Seneviratne J, Par
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127 14. Karaagaclioglu L, Can G, Yi
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129 28. Hahnel S, Rosentritt M, Han
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131 Tables Table 1 Table 1 - Median
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3.5 Capítulo 5 The effect of human
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135 Introduction An important step
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137 albicans adhesion to a denture
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139 For group G 3 , whole human uns
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141 each specimen, using a light mi
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143 was collected from one donor or
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145 In conclusion, this study focus
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147 11. Edgerton M, Scannapieco FA,
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149 24. Thein ZM, Samaranayake YH,
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151 37. Zamperini CA, Machado AL, V
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153 Figures Figure 1 *
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155 Figure Legends Figure 1. Mean a
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158 significantemente menores quand
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160 diminuindo os valores de ângul
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162 Nos estudos apresentados nos ca
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164 pela coloração cristal violet
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166 Diferentemente dos resultados o
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5 Conclusão Dentro das limitaçõe
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172 6. Bürgers R, Hahnel S, Reiche
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174 22. Hodak SK, Supasai T, Paosaw
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176 40. Nikawa H, Chen J, Hamada T,
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178 56. Pusateri CR, Monaco EA, Edg
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180 73. Tari BF, Nalbant D, Al DF,
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7 Anexos Anexo 1
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Anexo 3
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Anexo 5
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Anexo 7
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8 Apêndice Apêndice 1 Os resultad
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194 Tabela 3A - Grupo submetido ao
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Apêndice 2 Os resultados obtidos d
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224 Os resultados obtidos durante a
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