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3.2 Capítulo 2 Evaluation of fungal adherence to plasma-modified polymethylmethacrylate Fungal adherence to polymethylmethacrylate Zamperini CA a , Machado AL a, *, Vergani CE a , Pavarina AC a , Rangel EC b , Cruz NC b a Araraquara Dental School, UNESP – Univ Estadual Paulista, Department of Dental Materials and Prosthodontics, Araraquara, São Paulo, Rua Humaitá nº 1680, CEP 14.801-903, Brazil. b UNESP – Univ Estadual Paulista, Laboratory of Technological Plasmas, Sorocaba, São Paulo, Avenida 3 de Março nº 511, CEP 18.085-180, Brazil. *Corresponding author: Profa. Dra. Ana Lucia Machado Araraquara Dental School , UNESP –Univ. Estadual Paulista, Department of Dental Materials and Prosthodontics, Araraquara, São Paulo, Rua Humaitá nº 1680, CEP 14.801-903, Brazil. Tel: 55-16-33016410 Fax: 55-16-33016406 Email: cucci@foar.unesp.br Summary There is a propensity for fungal adherence to the polymethylmethacrylate used for making denture bases. Therefore, this study investigated whether surface modifications with plasma treatments would reduce the adherence of C. albicans to a denture base resin. Samples (n=180) with smooth and rough surfaces were
60 made and divided into five groups: control – non treated; experimental groups – submitted to plasma treatments to obtain surfaces with different hydrophobicities (Ar/50 W; ArO 2 /70 W; AAt/130 W) or with incorporated fluoride (Ar/SF 6 70 W). Contact angles were measured immediately after treatments and after samples were immersed in water for 48 hours. For each group, half the samples were incubated with saliva before the adherence test. The number of adhered C. albicans was evaluated by counting after violet crystal staining. The plasma treatments were effective in modifying the polymethylmethacrylate surface. However, there was a significant alteration in the contact angle measured after immersion in water. No statistically significant difference in the adherence of C. albicans was observed between the experimental and control groups, irrespective of the presence or absence of saliva, and surface roughness. Keywords: Candida spp; Candida albicans; fungal adherence; denture stomatitis; cell-surface-hydrophobicity. Introduction Polymers are widely used materials in different areas [1, 2]. In dentistry, polymethylmethacrylate is the polymer of choice for making removable denture bases for the purpose of rehabilitating partially or completely edentulous patients. Nevertheless, in spite of the good mechanical and esthetic properties of this material, microorganisms, particularly Candida albicans, have a propensity for
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UNIVERSIDADE ESTADUAL PAULISTA “J
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iii Dados Curriculares Camila Andra
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v exemplos, de quem eu me orgulho m
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vii Agradecimentos Especiais Muito
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Page 17 and 18: ii alterações significantes nos v
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Page 30 and 31: 3 Capítulos 3.1 Capítulo 1 Adhere
Page 32 and 33: 31 Introduction The inability of cu
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Page 36 and 37: 35 Plasma Treatments After roughnes
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Page 52 and 53: 51 33 Taylor R, Maryan C, Verran J.
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Page 84 and 85: 83 Tables Table 1 - Means and stand
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Page 98 and 99: 97 Statistical Analysis Differences
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Page 102 and 103: 101 One other important observation
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109 Tables Table 1 - Means and stan
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111 5.0 Log (cel/mm2) 4.0 3.0 2.0 *
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3.4 Capítulo 4 Effect of different
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115 Introduction The presence of Ca
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117 Italy) measuring 13.8 X 2 mm we
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119 4 - 6 ºC during the experiment
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121 Candida adhesion to dentures, a
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123 absorbance values obtained afte
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125 1. Silva WJ, Seneviratne J, Par
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127 14. Karaagaclioglu L, Can G, Yi
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129 28. Hahnel S, Rosentritt M, Han
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131 Tables Table 1 Table 1 - Median
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3.5 Capítulo 5 The effect of human
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135 Introduction An important step
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137 albicans adhesion to a denture
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139 For group G 3 , whole human uns
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141 each specimen, using a light mi
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143 was collected from one donor or
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145 In conclusion, this study focus
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147 11. Edgerton M, Scannapieco FA,
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149 24. Thein ZM, Samaranayake YH,
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151 37. Zamperini CA, Machado AL, V
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153 Figures Figure 1 *
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155 Figure Legends Figure 1. Mean a
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158 significantemente menores quand
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160 diminuindo os valores de ângul
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162 Nos estudos apresentados nos ca
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164 pela coloração cristal violet
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166 Diferentemente dos resultados o
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5 Conclusão Dentro das limitaçõe
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172 6. Bürgers R, Hahnel S, Reiche
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174 22. Hodak SK, Supasai T, Paosaw
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176 40. Nikawa H, Chen J, Hamada T,
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178 56. Pusateri CR, Monaco EA, Edg
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180 73. Tari BF, Nalbant D, Al DF,
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7 Anexos Anexo 1
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Anexo 3
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Anexo 5
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Anexo 7
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8 Apêndice Apêndice 1 Os resultad
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194 Tabela 3A - Grupo submetido ao
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Apêndice 2 Os resultados obtidos d
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224 Os resultados obtidos durante a
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