universidade de são paulo - Faculdade de Odontologia - Unesp

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was prepared in acetone at 0.4 mM immediately before each assay. After washing,
the specimens were transferred to new wells with 158 μl PBS with 200mM
glucose, 40 μl XTT and 2 μl menadione were inoculated to each well. The plates
were incubated for 3 h in the dark at 37 ºC 29 . The whole content of each well was
transferred to a tube, and centrifuged at 5000 g for 2 minutes. The colorimetric
change of the supernatant was measured using a microtiter plate reader (Thermo
Plate – TP Reader) at 492 nm.
Differences in the metabolic activity (XTT assay) among the experimental
conditions (smooth and rough surface, presence and absence of saliva), within
each group, was evaluated by Kruskal-Wallis test. Because no significant
differences were found, data from each group were then pooled together, and a
Kruskal-Wallis non-parametric analysis was performed to detect differences
among the groups. For each group, two-way repeated measure analysis of
variance, followed by Tukey’s test, was used to evaluate the effect of investing
technique and time of measurement on the contact angle. Data from roughness
measurements were analyzed by Kruskall-Wallis non-parametric test. A
significance level of 0.05 was used for all statistical tests.
Results
Candida albicans adherence as determined by XTT assay is shown in
Figure 1. Groups ArO 2 /70 W and ArSF 6 /70 W were not different from each other
and both showed significantly lower absorbance readings than the other groups
(p