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89 Abstract Candida adhesion to polymeric surfaces has been related to hydrophobic interactions. Objective: Therefore, this study evaluated if surface modifications with plasma treatments would reduce Candida glabrata adhesion to a denture base resin. Methods: Specimens (n=54) with smooth surfaces were made and divided into three groups (n=18): control – non-treated; experimental groups – specimens submitted to glow-discharge plasma treatment to obtain hydrophilic surfaces (Ar/50 W; AAt/130 W). Contact angles measurements were performed immediately after the treatments and after immersion in water for 48 h. For each group, half (n=9) of the specimens were precondicionated with saliva before the adhesion assay. The number of adhered C. glabrata was evaluated by counting after crystal violet staining. Results: Ar/50W group showed significantly lower C. glabrata adherence than the control group, in the absence of saliva. After preconditioning with saliva, C. glabrata adherence in experimental and control groups did not differ significantly. The plasma treatments were effective in modifying the acrylic surface. However, there were significant changes in the contact angles after 48 h of immersion in water. Conclusions: The results demonstrated that Ar/50 W plasma treatment showed promising potential for reducing C. glabrata adhesion to denture base resins. Keywords: Candida; Candida glabrata; saliva; acrylic resins.
90 Introduction The ability of Candida to grow attached to oral surfaces in communities known as biofilms is an important factor in the development of denture stomatitis. Although Candida albicans still is the microorganism most often associated with this infection, non-albicans species have been isolated from denture surfaces and oral mucosa [1]. Candida glabrata was the second most commonly isolated pathogen in patients with denture-induced stomatitis, followed by C. pseudotropicalis, C. Krusei, C. tropicalis, C. parapsilosis and others [1]. In addition, mixed Candida albicans and Candida glabrata biofilms have been associated with denture stomatitis [2], indicating that Candida glabrata may also play an integral role in this pathogenesis [2]. Moreover, in recent years, the prevalence of C. glabrata infections has increased, mainly in compromised patients [3]. This fact must receive attention because the mortality rate of C. glabrata infections is higher compared with infection with other non-albicans Candida and are more often difficult to treat [3]. Since the adhesion of Candida spp. to surfaces is a prerequisite for the formation of biofilm and development of denture stomatitis, the inhibition of this process could be effective to treat or prevent this pathology [4]. Many factors that affect Candida adherence have been described, among them the hydrophobic interactions. It has been demonstrated that these interactions are involved in the adherence of Candida to acrylic [4-8]. The closer the surface free energy of the substrate and the yeast, the higher was the probability of adherence [6]. A significant positive correlation between cell surface hydrophobicity and adhesion
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UNIVERSIDADE ESTADUAL PAULISTA “J
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iii Dados Curriculares Camila Andra
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v exemplos, de quem eu me orgulho m
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vii Agradecimentos Especiais Muito
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ix mais novo”! Se eu pudesse, tor
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xi Aos meus amigos de turma do curs
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xiii À Capes (Coordenação de Ape
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Sumário Resumo....................
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ii alterações significantes nos v
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ii adherence of Candida albicans, e
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21 como: não eliminação do micro
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23 mais hidrofóbica a superfície,
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25 2008), resultando em uma superf
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3 Capítulos 3.1 Capítulo 1 Adhere
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31 Introduction The inability of cu
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33 negatively charged surfaces 22 .
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35 Plasma Treatments After roughnes
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37 measurements were performed in a
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Page 46 and 47: 45 effect 27,30,42-44 or decreased
Page 48 and 49: 47 3 Yildirim MS, Hasanreisoglu U,
Page 50 and 51: 49 of Thai silk treated by SF 6 pla
Page 52 and 53: 51 33 Taylor R, Maryan C, Verran J.
Page 54 and 55: 53 47 Waters MGJ, Williams DW, Jagg
Page 56 and 57: 55 Table 2 - Means and standard dev
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Page 60 and 61: 3.2 Capítulo 2 Evaluation of funga
Page 62 and 63: 61 adhering to denture surfaces [1]
Page 64 and 65: 63 mimicking the tissue-fitting sur
Page 66 and 67: 65 spectroscopy analysis (XPS), car
Page 68 and 69: 67 suspension was added to each wel
Page 70 and 71: 69 Initial adherence of Candida alb
Page 72 and 73: 71 increase probably occurred by me
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Page 76 and 77: 75 3) For all groups evaluated, no
Page 78 and 79: 77 12. Pereira-Cenci T, Cury AADB,
Page 80 and 81: 79 25. Ramage G, Tomsett K, Wickes
Page 82 and 83: 81 38. Nikawa H, Hayashi S, Nikawa
Page 84 and 85: 83 Tables Table 1 - Means and stand
Page 86 and 87: 85 Figures Figure 1
Page 88 and 89: 87 Figure Legends Figure 1 - XPS an
Page 92 and 93: 91 to acrylic surfaces of Candida g
Page 94 and 95: 93 minutes. The denture base acryli
Page 96 and 97: 95 average was calculated. Specimen
Page 98 and 99: 97 Statistical Analysis Differences
Page 100 and 101: 99 the C. glabrata adhesion. The me
Page 102 and 103: 101 One other important observation
Page 104 and 105: 103 6. Minagi S, Miyake Y, Inagaki
Page 106 and 107: 105 19. Nikawa H, Hayashi S, Nikawa
Page 108 and 109: 107 33. Polukoshko KM, Brudvik JS,
Page 110 and 111: 109 Tables Table 1 - Means and stan
Page 112 and 113: 111 5.0 Log (cel/mm2) 4.0 3.0 2.0 *
Page 114 and 115: 3.4 Capítulo 4 Effect of different
Page 116 and 117: 115 Introduction The presence of Ca
Page 118 and 119: 117 Italy) measuring 13.8 X 2 mm we
Page 120 and 121: 119 4 - 6 ºC during the experiment
Page 122 and 123: 121 Candida adhesion to dentures, a
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Page 126 and 127: 125 1. Silva WJ, Seneviratne J, Par
Page 128 and 129: 127 14. Karaagaclioglu L, Can G, Yi
Page 130 and 131: 129 28. Hahnel S, Rosentritt M, Han
Page 132 and 133: 131 Tables Table 1 Table 1 - Median
Page 134 and 135: 3.5 Capítulo 5 The effect of human
Page 136 and 137: 135 Introduction An important step
Page 138 and 139: 137 albicans adhesion to a denture
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139 For group G 3 , whole human uns
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141 each specimen, using a light mi
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143 was collected from one donor or
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145 In conclusion, this study focus
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147 11. Edgerton M, Scannapieco FA,
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149 24. Thein ZM, Samaranayake YH,
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151 37. Zamperini CA, Machado AL, V
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153 Figures Figure 1 *
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155 Figure Legends Figure 1. Mean a
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158 significantemente menores quand
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160 diminuindo os valores de ângul
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162 Nos estudos apresentados nos ca
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164 pela coloração cristal violet
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166 Diferentemente dos resultados o
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5 Conclusão Dentro das limitaçõe
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172 6. Bürgers R, Hahnel S, Reiche
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174 22. Hodak SK, Supasai T, Paosaw
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176 40. Nikawa H, Chen J, Hamada T,
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178 56. Pusateri CR, Monaco EA, Edg
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180 73. Tari BF, Nalbant D, Al DF,
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7 Anexos Anexo 1
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Anexo 3
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Anexo 5
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Anexo 7
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8 Apêndice Apêndice 1 Os resultad
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194 Tabela 3A - Grupo submetido ao
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Apêndice 2 Os resultados obtidos d
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224 Os resultados obtidos durante a
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universidade de sÃ£o paulo - Faculdade de Odontologia - Unesp

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