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3.5 Capítulo 5 The effect of human whole saliva on the in vitro adhesion of Candida albicans to a denture base acrylic resin: a focus on collection and preparation of saliva samples Short title: Effect of human saliva on adhesion of Candida Zamperini CA a , Schiavinato PCS a , Pavarina AC a , Giampaolo ET a , Vergani CE a , Machado AL a, * a Araraquara Dental School, UNESP – Univ Estadual Paulista, Department of Dental Materials and Prosthodontics, Araraquara, São Paulo, Rua Humaitá nº 1680, CEP 14.801-903, Brazil. *Corresponding author: Profa. Dra. Ana Lucia Machado Araraquara Dental School, UNESP – Univ. Estadual Paulista, Department of Dental Materials and Prosthodontics, Araraquara, São Paulo, Rua Humaitá nº 1680, CEP 14.801-903, Brazil. Tel: 55-16-33016543; Fax: 55-16-33016406 Email: cucci@foar.unesp.br
134 Summary The effect of saliva on Candida albicans adhesion still remains controversial. The diverse protocols used for collection and preparation of saliva samples may contribute to the conflicting results. Thus, this study investigated whether variations in the centrifugation parameters and number of donors of saliva would influence the adhesion of C. albicans (ATCC 90028) to a denture base resin. Samples (n=72) with smooth surfaces were made and then divided into four groups: 1 control (C) - without saliva; 3 experimental groups – G1: saliva from 15 volunteers centrifuged at 10000 rpm for 5 min; G2: saliva from 15 volunteers centrifuged at 12000 rpm for 30 min; G3: saliva from 1 volunteer centrifuged at 10000 rpm for 5 min. Candida adhesion was evaluated by XTT reduction assay and crystal violet staining. Data were analyzed by one-way analyses of variance (P=0.05). For XTT assay, groups G 2 , G 3 and control were not significantly different, while group G 1 showed significantly higher absorbance value than control. For crystal violet staining, there were no significant differences among the mean log numbers of adherent cells for all groups. The results indicated that variations in the centrifugation parameters and number of donors may influence the effect of saliva on Candida albicans adhesion to denture base resins. Key words: Acrylic Resins; Biofilms; Candida; Candida albicans; Saliva.
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UNIVERSIDADE ESTADUAL PAULISTA “J
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iii Dados Curriculares Camila Andra
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v exemplos, de quem eu me orgulho m
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vii Agradecimentos Especiais Muito
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ix mais novo”! Se eu pudesse, tor
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xi Aos meus amigos de turma do curs
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xiii À Capes (Coordenação de Ape
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Sumário Resumo....................
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ii alterações significantes nos v
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ii adherence of Candida albicans, e
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21 como: não eliminação do micro
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23 mais hidrofóbica a superfície,
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25 2008), resultando em uma superf
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3 Capítulos 3.1 Capítulo 1 Adhere
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31 Introduction The inability of cu
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33 negatively charged surfaces 22 .
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35 Plasma Treatments After roughnes
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37 measurements were performed in a
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39 was prepared in acetone at 0.4 m
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41 forces, the development of the m
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43 adherence observed in the presen
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45 effect 27,30,42-44 or decreased
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47 3 Yildirim MS, Hasanreisoglu U,
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49 of Thai silk treated by SF 6 pla
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51 33 Taylor R, Maryan C, Verran J.
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53 47 Waters MGJ, Williams DW, Jagg
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55 Table 2 - Means and standard dev
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Figure 2 57
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3.2 Capítulo 2 Evaluation of funga
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61 adhering to denture surfaces [1]
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63 mimicking the tissue-fitting sur
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65 spectroscopy analysis (XPS), car
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67 suspension was added to each wel
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69 Initial adherence of Candida alb
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71 increase probably occurred by me
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73 to the adsorption of salivary pr
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75 3) For all groups evaluated, no
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77 12. Pereira-Cenci T, Cury AADB,
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79 25. Ramage G, Tomsett K, Wickes
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81 38. Nikawa H, Hayashi S, Nikawa
Page 84 and 85: 83 Tables Table 1 - Means and stand
Page 86 and 87: 85 Figures Figure 1
Page 88 and 89: 87 Figure Legends Figure 1 - XPS an
Page 90 and 91: 89 Abstract Candida adhesion to pol
Page 92 and 93: 91 to acrylic surfaces of Candida g
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Page 96 and 97: 95 average was calculated. Specimen
Page 98 and 99: 97 Statistical Analysis Differences
Page 100 and 101: 99 the C. glabrata adhesion. The me
Page 102 and 103: 101 One other important observation
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Page 106 and 107: 105 19. Nikawa H, Hayashi S, Nikawa
Page 108 and 109: 107 33. Polukoshko KM, Brudvik JS,
Page 110 and 111: 109 Tables Table 1 - Means and stan
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Page 114 and 115: 3.4 Capítulo 4 Effect of different
Page 116 and 117: 115 Introduction The presence of Ca
Page 118 and 119: 117 Italy) measuring 13.8 X 2 mm we
Page 120 and 121: 119 4 - 6 ºC during the experiment
Page 122 and 123: 121 Candida adhesion to dentures, a
Page 124 and 125: 123 absorbance values obtained afte
Page 126 and 127: 125 1. Silva WJ, Seneviratne J, Par
Page 128 and 129: 127 14. Karaagaclioglu L, Can G, Yi
Page 130 and 131: 129 28. Hahnel S, Rosentritt M, Han
Page 132 and 133: 131 Tables Table 1 Table 1 - Median
Page 136 and 137: 135 Introduction An important step
Page 138 and 139: 137 albicans adhesion to a denture
Page 140 and 141: 139 For group G 3 , whole human uns
Page 142 and 143: 141 each specimen, using a light mi
Page 144 and 145: 143 was collected from one donor or
Page 146 and 147: 145 In conclusion, this study focus
Page 148 and 149: 147 11. Edgerton M, Scannapieco FA,
Page 150 and 151: 149 24. Thein ZM, Samaranayake YH,
Page 152 and 153: 151 37. Zamperini CA, Machado AL, V
Page 154 and 155: 153 Figures Figure 1 *
Page 156: 155 Figure Legends Figure 1. Mean a
Page 159 and 160: 158 significantemente menores quand
Page 161 and 162: 160 diminuindo os valores de ângul
Page 163 and 164: 162 Nos estudos apresentados nos ca
Page 165 and 166: 164 pela coloração cristal violet
Page 167 and 168: 166 Diferentemente dos resultados o
Page 170: 5 Conclusão Dentro das limitaçõe
Page 173 and 174: 172 6. Bürgers R, Hahnel S, Reiche
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Page 179 and 180: 178 56. Pusateri CR, Monaco EA, Edg
Page 181 and 182: 180 73. Tari BF, Nalbant D, Al DF,
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7 Anexos Anexo 1
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Anexo 3
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Anexo 5
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Anexo 7
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8 Apêndice Apêndice 1 Os resultad
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194 Tabela 3A - Grupo submetido ao
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Apêndice 2 Os resultados obtidos d
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224 Os resultados obtidos durante a
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