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universidade de são paulo - Faculdade de Odontologia - Unesp

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145<br />

In conclusion, this study focused mainly on the effect of variations in the<br />

collection and preparation of saliva on the Candida albicans adhesion. The results<br />

<strong>de</strong>monstrated that variations in the centrifugation parameters and number of<br />

donors can influence the effect of saliva on Candida albicans adhesion, thus<br />

emphasizing that the protocols must be standardized. As the present study<br />

evaluated only one <strong>de</strong>nture base acrylic resin, further investigations with other<br />

materials are necessary. Moreover, other Candida species must also be evaluated<br />

in future studies. Besi<strong>de</strong>s, in addition to the speed and time of centrifugation and<br />

number of donors, the use of filtered or unfiltered saliva has varied among studies<br />

and the influence of this difference on Candida adhesion to <strong>de</strong>nture materials<br />

needs further investigations.<br />

Acknowledgements<br />

This research was supported by FAPESP (Grant - 2008/05339-8) and<br />

CNPq (Grant - 479895/2009-0).<br />

References<br />

1. Klotz SA, Drutz DJ, Zajic JE. Factors governing adherence of Candida<br />

species to plastic surfaces. Infect Immun. 1985; 50 (1): 97-101.<br />

2. Radford DR, Challacombe SJ, Walter JD. Denture plaque and adherence<br />

of Candida albicans to <strong>de</strong>nture-base materials in vivo and in vitro. Crit<br />

Rev Oral Biol Med. 1999; 10 (1): 99-116.<br />

3. Verran J, Maryan CJ. Retention of Candida albicans on acrylic resin<br />

and silicone of different surface topography. J Prosthet Dent. 1997; 77:<br />

535-9.

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