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75 3) For all groups evaluated, no significant effect was detected with regard to the influence of surface roughness and saliva on the adherence of Candida albicans. Acknowledgements This research was supported by FAPESP (Grant - 2007/02210-1 and 2007/04917-5) and CNPq (Grant 479252/2007-6). We thank Prof. Peter Hammer for his assistance with the XPS analysis. References 1. Shmack G, Dutschk V, Pisanova E. Modification of polyamide fibres to improve their biocompatibility. Fibre Chemistry 2000; 32: 48-55. 2. Lai J, Sunderland B, Xue J, Yan S, Zhao W, Folkard M, Michael BD, Wang Y. Study of hydrophilicity of polymer surfaces improved by plasma treatment. Appl Surf Sci 2006; 252: 3375-3379. 3. Verran J, Maryan CJ. Retention of Candida albicans on acrylic resin and silicone of different surface topography. J Prosthet Dent 1997; 77: 535-539. 4. Yildirim MS, Hasanreisoglu U, Hasirci N, Sultan N. Adherence of Candida albicans to glow-discharge modified acrylic denture base polymers. J Oral Rehabil 2005; 32: 518-525. 5. Nikawa H, Yamamoto T, Hamada T, Rahardjo MB, Murata H. Antifungal effect of zeolite-incorporated tissue conditioner against
76 Candida albicans growth and/or acid production. J Oral Rehabil 1997; 24: 350-357. 6. Dagistan S, Aktas AE, Caglayan F, Ayyildiz A, Bilge M. Differential diagnosis of denture-induced stomatitis, Candida, and their variations in patients using complete denture: a clinical and mycological study. Mycoses 2009; 52: 266-271. 7. Henriques M, Azeredo J, Oliveira R. Adhesion of Candida albicans and Candida dubliniensis to acrylic and hydroxyapatite. Colloids Surf B Biointerfaces 2004; 33: 235-241. 8. Samaranayake LP, McCourtie J, MacFarlane TW. Factors affecting the in-vitro adherence of Candida albicans to acrylic surfaces. Arch Oral Biol 1980; 25: 611-615. 9. Pereira-Cenci T, Cury AADB, Cenci MS, Rodrigues- Garcia RCM. In vitro Candida colonization on acrylic resins and denture liners: influence of surface free energy, roughness, saliva, and adhering bacteria. Int J Prosthodont 2007; 20: 308-310. 10. Moura JS, Silva WJ, Pereira T, Cury AADB, Garcia RCMR. Influence of acrylic resin polymerization methods and saliva on the adherence of four Candida species. J Prosthet Dent 2006; 96: 205-211. 11. Park SE, Periathamby AR, Loza JC. Effect of surfacecharged poly(methylmethacrylate) on the adhesion of Candida albicans. J Prosthodont 2003; 12: 249-254.
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UNIVERSIDADE ESTADUAL PAULISTA “J
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iii Dados Curriculares Camila Andra
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v exemplos, de quem eu me orgulho m
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vii Agradecimentos Especiais Muito
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ix mais novo”! Se eu pudesse, tor
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xi Aos meus amigos de turma do curs
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xiii À Capes (Coordenação de Ape
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Sumário Resumo....................
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ii alterações significantes nos v
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ii adherence of Candida albicans, e
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21 como: não eliminação do micro
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23 mais hidrofóbica a superfície,
Page 26: 25 2008), resultando em uma superf
Page 30 and 31: 3 Capítulos 3.1 Capítulo 1 Adhere
Page 32 and 33: 31 Introduction The inability of cu
Page 34 and 35: 33 negatively charged surfaces 22 .
Page 36 and 37: 35 Plasma Treatments After roughnes
Page 38 and 39: 37 measurements were performed in a
Page 40 and 41: 39 was prepared in acetone at 0.4 m
Page 42 and 43: 41 forces, the development of the m
Page 44 and 45: 43 adherence observed in the presen
Page 46 and 47: 45 effect 27,30,42-44 or decreased
Page 48 and 49: 47 3 Yildirim MS, Hasanreisoglu U,
Page 50 and 51: 49 of Thai silk treated by SF 6 pla
Page 52 and 53: 51 33 Taylor R, Maryan C, Verran J.
Page 54 and 55: 53 47 Waters MGJ, Williams DW, Jagg
Page 56 and 57: 55 Table 2 - Means and standard dev
Page 58 and 59: Figure 2 57
Page 60 and 61: 3.2 Capítulo 2 Evaluation of funga
Page 62 and 63: 61 adhering to denture surfaces [1]
Page 64 and 65: 63 mimicking the tissue-fitting sur
Page 66 and 67: 65 spectroscopy analysis (XPS), car
Page 68 and 69: 67 suspension was added to each wel
Page 70 and 71: 69 Initial adherence of Candida alb
Page 72 and 73: 71 increase probably occurred by me
Page 74 and 75: 73 to the adsorption of salivary pr
Page 78 and 79: 77 12. Pereira-Cenci T, Cury AADB,
Page 80 and 81: 79 25. Ramage G, Tomsett K, Wickes
Page 82 and 83: 81 38. Nikawa H, Hayashi S, Nikawa
Page 84 and 85: 83 Tables Table 1 - Means and stand
Page 86 and 87: 85 Figures Figure 1
Page 88 and 89: 87 Figure Legends Figure 1 - XPS an
Page 90 and 91: 89 Abstract Candida adhesion to pol
Page 92 and 93: 91 to acrylic surfaces of Candida g
Page 94 and 95: 93 minutes. The denture base acryli
Page 96 and 97: 95 average was calculated. Specimen
Page 98 and 99: 97 Statistical Analysis Differences
Page 100 and 101: 99 the C. glabrata adhesion. The me
Page 102 and 103: 101 One other important observation
Page 104 and 105: 103 6. Minagi S, Miyake Y, Inagaki
Page 106 and 107: 105 19. Nikawa H, Hayashi S, Nikawa
Page 108 and 109: 107 33. Polukoshko KM, Brudvik JS,
Page 110 and 111: 109 Tables Table 1 - Means and stan
Page 112 and 113: 111 5.0 Log (cel/mm2) 4.0 3.0 2.0 *
Page 114 and 115: 3.4 Capítulo 4 Effect of different
Page 116 and 117: 115 Introduction The presence of Ca
Page 118 and 119: 117 Italy) measuring 13.8 X 2 mm we
Page 120 and 121: 119 4 - 6 ºC during the experiment
Page 122 and 123: 121 Candida adhesion to dentures, a
Page 124 and 125: 123 absorbance values obtained afte
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125 1. Silva WJ, Seneviratne J, Par
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127 14. Karaagaclioglu L, Can G, Yi
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129 28. Hahnel S, Rosentritt M, Han
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131 Tables Table 1 Table 1 - Median
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3.5 Capítulo 5 The effect of human
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135 Introduction An important step
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137 albicans adhesion to a denture
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139 For group G 3 , whole human uns
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141 each specimen, using a light mi
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143 was collected from one donor or
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145 In conclusion, this study focus
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147 11. Edgerton M, Scannapieco FA,
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149 24. Thein ZM, Samaranayake YH,
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151 37. Zamperini CA, Machado AL, V
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153 Figures Figure 1 *
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155 Figure Legends Figure 1. Mean a
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158 significantemente menores quand
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160 diminuindo os valores de ângul
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162 Nos estudos apresentados nos ca
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164 pela coloração cristal violet
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166 Diferentemente dos resultados o
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5 Conclusão Dentro das limitaçõe
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172 6. Bürgers R, Hahnel S, Reiche
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174 22. Hodak SK, Supasai T, Paosaw
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176 40. Nikawa H, Chen J, Hamada T,
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178 56. Pusateri CR, Monaco EA, Edg
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180 73. Tari BF, Nalbant D, Al DF,
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7 Anexos Anexo 1
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Anexo 3
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Anexo 5
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Anexo 7
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8 Apêndice Apêndice 1 Os resultad
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194 Tabela 3A - Grupo submetido ao
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Apêndice 2 Os resultados obtidos d
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224 Os resultados obtidos durante a
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