universidade de sÃ£o paulo - Faculdade de Odontologia - Unesp

More documents

Recommendations

Info

115 Introduction The presence of Candida albicans biofilms on removable denture surfaces plays an important role in the etiology of denture stomatitis. The capacity of this fungus to adhere to surfaces is the first stage in the biofilm formation process, which is followed by colony formation and cell organization, secretion of extracellular matrix, maturation and dissemination of the biofilm 1 . It is known that the adhesion of Candida cells to denture surfaces is mediated by a salivary conditioning film or pellicle 2,3,4 that provides receptors for microbial adhesion 5 . However, the role of saliva on Candida albicans adhesion to biomaterials is, as yet, not fully established, as conflicting results have been reported. Some authors have observed that the saliva pellicle promoted Candida albicans colonization on the materials 6,7,8,9,10 . Conversely, others have found that preconditioning the materials with saliva either did not affect 11,12,13,14 or reduced Candida albicans adhesion 15,16,17,18,19 . The different periods of preconditioning with saliva could interfere in the adhesive capacity of Candida 20 and contribute to the inconsistent findings from these studies. In a number of researches, the materials were incubated with saliva for short periods such as 30 minutes 16,17,18,19 , 1 hour 7,8,9 , 2 to 3 hours 4,10 and 4 hours 11,13 . Longer incubation periods, including overnight 21 , 18 hours 15 and 24 hours 22 have also been used to create a salivary conditioning film. However, to date, no information is available concerning how different periods of preconditioning with saliva affect the adhesion of Candida cells to denture materials.
116 methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) One method used for evaluating adhesion of Candida is the cell staining assay 15,18,19,23,24 . In this method, the adhered cells on the material surface are fixed, stained and counted in a microscope. The dye commonly used in this technique is the crystal violet, which is basic and binds to negatively charged extracellular molecules, including cell surface molecules and polysaccharides in the extracellular matrices 25 . Another method that has been widely used is the XTT assay 1,10,11,16 , which evaluates the metabolic activity of viable cells 26 . This assay is based on the reduction of a water-soluble tetrazolium salt [2,3-bis(2- carbonyl]-2H-tetrazoliumhydroxide] to a formazan product by the mitochondrial dehydrogenases of the live cells. Therefore, the aim of this study was to assess the effect of different periods of preconditioning with saliva on the adhesion of C. albicans to a denture base resin using crystal violet staining and XTT assay. Additionally, the correlation between the two methods used for assessing C. albicans adhesion was evaluated. Materials and Methods Preparation of acrylic resin specimens The specimens (n=90) were fabricated from a microwave denture-base acrylic resin (Vipi Wave - VIPI Indústria e Comércio Exportação e Importação de Produtos Odontológicos Ltda Pirassununga, SP, Brazil) using a conventional flasking and pressure-pack technique. Initially, with the use of a metal mold, diskshaped silicone patterns Zetaplus/Indurent - Zhermack, Badia Polesine, Rovigo,
Page 1 and 2:
UNIVERSIDADE ESTADUAL PAULISTA “J
Page 3 and 4:
iii Dados Curriculares Camila Andra
Page 5 and 6:
v exemplos, de quem eu me orgulho m
Page 7 and 8:
vii Agradecimentos Especiais Muito
Page 9 and 10:
ix mais novo”! Se eu pudesse, tor
Page 11 and 12:
xi Aos meus amigos de turma do curs
Page 13 and 14:
xiii À Capes (Coordenação de Ape
Page 15 and 16:
Sumário Resumo....................
Page 17 and 18:
ii alterações significantes nos v
Page 19:
ii adherence of Candida albicans, e
Page 22 and 23:
21 como: não eliminação do micro
Page 24 and 25:
23 mais hidrofóbica a superfície,
Page 26:
25 2008), resultando em uma superf
Page 30 and 31:
3 Capítulos 3.1 Capítulo 1 Adhere
Page 32 and 33:
31 Introduction The inability of cu
Page 34 and 35:
33 negatively charged surfaces 22 .
Page 36 and 37:
35 Plasma Treatments After roughnes
Page 38 and 39:
37 measurements were performed in a
Page 40 and 41:
39 was prepared in acetone at 0.4 m
Page 42 and 43:
41 forces, the development of the m
Page 44 and 45:
43 adherence observed in the presen
Page 46 and 47:
45 effect 27,30,42-44 or decreased
Page 48 and 49:
47 3 Yildirim MS, Hasanreisoglu U,
Page 50 and 51:
49 of Thai silk treated by SF 6 pla
Page 52 and 53:
51 33 Taylor R, Maryan C, Verran J.
Page 54 and 55:
53 47 Waters MGJ, Williams DW, Jagg
Page 56 and 57:
55 Table 2 - Means and standard dev
Page 58 and 59:
Figure 2 57
Page 60 and 61:
3.2 Capítulo 2 Evaluation of funga
Page 62 and 63:
61 adhering to denture surfaces [1]
Page 64 and 65:
63 mimicking the tissue-fitting sur
Page 66 and 67: 65 spectroscopy analysis (XPS), car
Page 68 and 69: 67 suspension was added to each wel
Page 70 and 71: 69 Initial adherence of Candida alb
Page 72 and 73: 71 increase probably occurred by me
Page 74 and 75: 73 to the adsorption of salivary pr
Page 76 and 77: 75 3) For all groups evaluated, no
Page 78 and 79: 77 12. Pereira-Cenci T, Cury AADB,
Page 80 and 81: 79 25. Ramage G, Tomsett K, Wickes
Page 82 and 83: 81 38. Nikawa H, Hayashi S, Nikawa
Page 84 and 85: 83 Tables Table 1 - Means and stand
Page 86 and 87: 85 Figures Figure 1
Page 88 and 89: 87 Figure Legends Figure 1 - XPS an
Page 90 and 91: 89 Abstract Candida adhesion to pol
Page 92 and 93: 91 to acrylic surfaces of Candida g
Page 94 and 95: 93 minutes. The denture base acryli
Page 96 and 97: 95 average was calculated. Specimen
Page 98 and 99: 97 Statistical Analysis Differences
Page 100 and 101: 99 the C. glabrata adhesion. The me
Page 102 and 103: 101 One other important observation
Page 104 and 105: 103 6. Minagi S, Miyake Y, Inagaki
Page 106 and 107: 105 19. Nikawa H, Hayashi S, Nikawa
Page 108 and 109: 107 33. Polukoshko KM, Brudvik JS,
Page 110 and 111: 109 Tables Table 1 - Means and stan
Page 112 and 113: 111 5.0 Log (cel/mm2) 4.0 3.0 2.0 *
Page 114 and 115: 3.4 Capítulo 4 Effect of different
Page 118 and 119: 117 Italy) measuring 13.8 X 2 mm we
Page 120 and 121: 119 4 - 6 ºC during the experiment
Page 122 and 123: 121 Candida adhesion to dentures, a
Page 124 and 125: 123 absorbance values obtained afte
Page 126 and 127: 125 1. Silva WJ, Seneviratne J, Par
Page 128 and 129: 127 14. Karaagaclioglu L, Can G, Yi
Page 130 and 131: 129 28. Hahnel S, Rosentritt M, Han
Page 132 and 133: 131 Tables Table 1 Table 1 - Median
Page 134 and 135: 3.5 Capítulo 5 The effect of human
Page 136 and 137: 135 Introduction An important step
Page 138 and 139: 137 albicans adhesion to a denture
Page 140 and 141: 139 For group G 3 , whole human uns
Page 142 and 143: 141 each specimen, using a light mi
Page 144 and 145: 143 was collected from one donor or
Page 146 and 147: 145 In conclusion, this study focus
Page 148 and 149: 147 11. Edgerton M, Scannapieco FA,
Page 150 and 151: 149 24. Thein ZM, Samaranayake YH,
Page 152 and 153: 151 37. Zamperini CA, Machado AL, V
Page 154 and 155: 153 Figures Figure 1 *
Page 156: 155 Figure Legends Figure 1. Mean a
Page 159 and 160: 158 significantemente menores quand
Page 161 and 162: 160 diminuindo os valores de ângul
Page 163 and 164: 162 Nos estudos apresentados nos ca
Page 165 and 166: 164 pela coloração cristal violet
Page 167 and 168:
166 Diferentemente dos resultados o
Page 170:
5 Conclusão Dentro das limitaçõe
Page 173 and 174:
172 6. Bürgers R, Hahnel S, Reiche
Page 175 and 176:
174 22. Hodak SK, Supasai T, Paosaw
Page 177 and 178:
176 40. Nikawa H, Chen J, Hamada T,
Page 179 and 180:
178 56. Pusateri CR, Monaco EA, Edg
Page 181 and 182:
180 73. Tari BF, Nalbant D, Al DF,
Page 184 and 185:
7 Anexos Anexo 1
Page 186 and 187:
Anexo 3
Page 188 and 189:
Anexo 5
Page 190 and 191:
Anexo 7
Page 193 and 194:
8 Apêndice Apêndice 1 Os resultad
Page 195 and 196:
194 Tabela 3A - Grupo submetido ao
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Apêndice 2 Os resultados obtidos d
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
224 Os resultados obtidos durante a
Page 227:
Autorizo a reprodução deste traba
show all

universidade de sÃ£o paulo - Faculdade de Odontologia - Unesp

Create successful ePaper yourself

Delete template?

Save as template?