universidade de são paulo - Faculdade de Odontologia - Unesp

122
were no significant differences in the mean roughness values among all groups
evaluated, for both crystal violet staining and XTT assay (Table 1).
The results from crystal violet staining and XTT assay revealed that the
differences among all experimental groups were not significant, indicating that the
periods of preconditioning with saliva evaluated in the present investigation did
not influence the adhesion of Candida albicans (Table 2). These findings suggest
that in studies of Candida albicans adhesion to acrylic resins, shorter periods of
preconditioning with saliva, such as 30 or 60 minutes could be used, making the
adhesion assay procedures easier and quicker to perform. Nevertheless, it is
important to point out that the saliva used in this study was filter sterilized 27 to
obtain more reproducible results 17 . Given that salivary pellicle formation on
biomaterials is a selective process 31 , the filtration process may have depleted
some of the protein complexes, such as certain mucin complexes involved in
Candida adhesion, that are present in human saliva 17 . Therefore, further studies
should be conducted to evaluate the influence of different periods of
preconditioning the substrates on Candida adhesion, when using whole saliva.
The coefficient of correlation between crystal violet staining and XTT
assay was low (r=0.223, P = 0.141), showing no significant correlation between
the two methods used for assessing C. albicans adherence. The results obtained by
staining demonstrated that there were no significant differences between the
experimental groups and the control, indicating that preconditioning with saliva
did not influence the fungal adhesion (Table 2). For XTT assay, although the
statistical analysis failed to find significant differences among the groups, the