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Zamperini CA. Effects of different plasma treatments and variations in the collection, preparation and preconditioning with saliva on Candida adhesion to a denture base acrylic resin [Tese de Doutorado]. Araraquara: Faculdade de Odontologia da UNESP; 2011. Abstract The adhesion of Candida to denture surfaces is the initial step for occurrence of denture stomatitis. Among the various factors involved on Candida adhesion to polymeric surfaces are the hydrophobic and eletrostatic interactions, surface roughness and pellicle salivary. Hence, the aims of this study were: to investigate the potential of different plasma treatments (Ar/50W; ArO 2 /70W; AAt/130W; ArSF 6 /70W) to modify a denture base acrylic resin (VIPIWAVE) to reduce the Candida albicans adhesion (ATCC 90028), evaluated by XTT reduction assay and crystal violet staining. The effect of surface roughness and saliva coating was also evaluated; to investigate the potential of two plasma treatments (Ar/50W; AAt/130W) to modify a denture base acrylic resin to reduce the Candida glabrata adhesion (ATCC 2001), evaluated by crystal violet staining. Moreover, the effect of saliva coating was also evaluated; and to assess the effect of different periods of preconditioning with saliva (0 min; 30 min; 60 min; 180 min; 720 min), variations in the centrifugation parameters (speed and time) and number of donors of saliva on Candida albicans adhesion to a denture base resin using crystal violet staining and XTT reduction assay. Additionally, the correlation between the two methods used for assessing Candida albicans adhesion was also evaluated. The results obtained demonstrated that the plasma treatments were effective in modifying hydrophobicity or incorporation of fluorine into acrylic resin. However, there were significant alterations in the contact angle measured after immersion in water. Groups ArO 2 /70W and ArSF 6 /70W showed significantly lower absorbance readings to Candida albicans adhesion than the other groups. No statistically significant difference in the
ii adherence of Candida albicans, evaluated by crystal violet staining, was observed between the plasma treated and control groups, irrespective of the presence or absence of saliva, and surface roughness. The number of adhered Candida glabrata, evaluated by counting after crystal violet staining, was significantly lower in Ar/50W group than the control group, in the absence of saliva. However, after preconditioning with saliva, Candida glabrata adherence in experimental and control groups did not differ significantly. The different periods of preconditioning with saliva had no significant influence in the Candida albicans adhesion, but the centrifugation parameters (speed and time) and number of donors of saliva influenced the results of Candida albicans adhesion to the denture base acrylic resin. No significant correlation was found between the two methods used for assessing Candida albicans adhesion, crystal violet staining and XTT reduction method. Thus, the results demonstrated that ArO 2 /70W and ArSF 6 /70W plasma treatments showed promising potential for reducing Candida albicans adhesion, while the Ar/50W plasma treatment showed promising potential for reducing Candida glabrata adhesion to denture base resins. Moreover, the saliva pellicle, depending of experimental conditions, may increase the Candida albicans adhesion, but it not significantly influences the Candida glabrata adhesion. The diverse methodological procedures regarding to preconditioning with saliva alter the results of Candida albicans adhesion. Keywords: Cell adherence; biofilms; Candida albicans; Candida glabrata; acrylic resins; saliva.
Page 1 and 2: UNIVERSIDADE ESTADUAL PAULISTA “J
Page 3 and 4: iii Dados Curriculares Camila Andra
Page 5 and 6: v exemplos, de quem eu me orgulho m
Page 7 and 8: vii Agradecimentos Especiais Muito
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Page 17: ii alterações significantes nos v
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Page 26: 25 2008), resultando em uma superf
Page 30 and 31: 3 Capítulos 3.1 Capítulo 1 Adhere
Page 32 and 33: 31 Introduction The inability of cu
Page 34 and 35: 33 negatively charged surfaces 22 .
Page 36 and 37: 35 Plasma Treatments After roughnes
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67 suspension was added to each wel
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69 Initial adherence of Candida alb
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71 increase probably occurred by me
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73 to the adsorption of salivary pr
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75 3) For all groups evaluated, no
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77 12. Pereira-Cenci T, Cury AADB,
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79 25. Ramage G, Tomsett K, Wickes
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81 38. Nikawa H, Hayashi S, Nikawa
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83 Tables Table 1 - Means and stand
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85 Figures Figure 1
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87 Figure Legends Figure 1 - XPS an
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89 Abstract Candida adhesion to pol
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91 to acrylic surfaces of Candida g
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93 minutes. The denture base acryli
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95 average was calculated. Specimen
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97 Statistical Analysis Differences
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99 the C. glabrata adhesion. The me
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101 One other important observation
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103 6. Minagi S, Miyake Y, Inagaki
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105 19. Nikawa H, Hayashi S, Nikawa
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107 33. Polukoshko KM, Brudvik JS,
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109 Tables Table 1 - Means and stan
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111 5.0 Log (cel/mm2) 4.0 3.0 2.0 *
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3.4 Capítulo 4 Effect of different
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115 Introduction The presence of Ca
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117 Italy) measuring 13.8 X 2 mm we
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119 4 - 6 ºC during the experiment
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121 Candida adhesion to dentures, a
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123 absorbance values obtained afte
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125 1. Silva WJ, Seneviratne J, Par
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127 14. Karaagaclioglu L, Can G, Yi
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129 28. Hahnel S, Rosentritt M, Han
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131 Tables Table 1 Table 1 - Median
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3.5 Capítulo 5 The effect of human
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135 Introduction An important step
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137 albicans adhesion to a denture
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139 For group G 3 , whole human uns
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141 each specimen, using a light mi
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143 was collected from one donor or
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145 In conclusion, this study focus
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147 11. Edgerton M, Scannapieco FA,
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149 24. Thein ZM, Samaranayake YH,
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151 37. Zamperini CA, Machado AL, V
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153 Figures Figure 1 *
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155 Figure Legends Figure 1. Mean a
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158 significantemente menores quand
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160 diminuindo os valores de ângul
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162 Nos estudos apresentados nos ca
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164 pela coloração cristal violet
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166 Diferentemente dos resultados o
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5 Conclusão Dentro das limitaçõe
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172 6. Bürgers R, Hahnel S, Reiche
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174 22. Hodak SK, Supasai T, Paosaw
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176 40. Nikawa H, Chen J, Hamada T,
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178 56. Pusateri CR, Monaco EA, Edg
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180 73. Tari BF, Nalbant D, Al DF,
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7 Anexos Anexo 1
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Anexo 3
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Anexo 5
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Anexo 7
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8 Apêndice Apêndice 1 Os resultad
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194 Tabela 3A - Grupo submetido ao
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Apêndice 2 Os resultados obtidos d
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224 Os resultados obtidos durante a
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