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universidade de são paulo - Faculdade de Odontologia - Unesp

universidade de são paulo - Faculdade de Odontologia - Unesp

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93<br />

minutes. The <strong>de</strong>nture base acrylic resin was processed in a 500 W domestic<br />

microwave oven (Brastemp – Brastemp Amazonia SA, Manaus, AM, Brazil) for<br />

20 minutes at 20% power, followed by 5 minutes at 90% power. The flasks were<br />

allowed to bench cool at room temperature, the specimens were <strong>de</strong>flasked, and<br />

excess flash was aseptically removed with a sterile bur (Maxi-Cut; Lesfils <strong>de</strong><br />

August Malleifer SA, Ballaigues, Switzerland).<br />

Surface roughness measurements<br />

The surface roughness of all specimens was measured with a profilometer<br />

(Mitutoyo SJ 400 – Mitutoyo Corporation – Tokyo, Japan). Four measurements<br />

were ma<strong>de</strong> for each specimen and the average reading was <strong>de</strong>signated as the Ra<br />

(μm) value of that specimen. Resolution was 0.01 μm, interval (cutoff length) was<br />

0.8 mm, transverse length was 2.4 mm, the stylus speed was 0.5 mm/s, and the<br />

diamond stylus tip radius was 5 μm. All measurements were recor<strong>de</strong>d by one<br />

operator.<br />

Plasma treatments<br />

After roughness measurements, the specimens were cleaned in ultrasonic<br />

water and <strong>de</strong>tergent bath for 15 minutes, then sonicated in distilled water for 15<br />

minutes and dried in air. The specimens were then divi<strong>de</strong>d into three groups, each<br />

one including 18 specimens. In the control group, the specimens were left<br />

untreated. For the two experimental groups, both specimen surfaces were exposed<br />

to generated plasmas using the following conditions: argon atmosphere at 50 W<br />

(group Ar/50 W); atmospheric air at 130 W (AAt/130 W). The plasma exposure

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