Journal Thoracic Oncology

Abstracts Journal of Thoracic Oncology • Volume 12 Issue S1 January 2017
POSTER SESSION 2 – P2.05: RADIOTHERAPY
MULTIMODALITY TREATMENT –
TUESDAY, DECEMBER 6, 2016
P2.05-027 EFFECTS OF THERMO-CHEMOTHERAPY FOR LUNG
CANCER INDUCED BY NANO-PACLITAXEL MAGNETIC FLUID
Runlei Hu 1 , Hong Jiang 1 , Dongshan Wei 1 , Shenglin Ma 2
1 Thoracic Surgery, Hangzhou First People’s Hospital, Hangzhou/China, 2 Hangzhou
First People’s Hospital, Hangzhou/China
Background: The aim of this study was to investigated the effects of thermochemotherapy
induced by nano-paclitaxel magnetic fluid for lung cancer
A549 proliferation, apoptosis and cell cycle in vitro, and therapeutic effect
of human carcinoma A549 xenograft in nude mice in vivo. Methods: In vitro,
nano-paclitaxel magnetic liquid was synthesised by chemical coprecipitation
and ultrasound emulsification. Lung cancer A549 cells were set up the control
group (group A), thermal therapy group (group B), chemotherapy group
(group C) and thermo-chemotherapy group (group D), which exposed to an
alternative magnetic field (AMF) for 30 min. And then the optical density (OD)
of viable cell, cytotocixity index, growth curve of cells, morphologic changes
of cell, cell cycle and aposptosis were measured. When tumor length to
diameter (6 ~ 8 mm), they were randomly divided into 4 groups: control group,
magnetic heat treatment group, paclitaxel magnetic thermo-chemotherapy
group and chemotherapy group, the tumor was heated in an AMF for 30 min.
Tumor volumes were then measured every week. The therapeutic effect
was assessed by measuring the tumor volume and weight. Pathological
examination was performed with a light microscope following treatment.
Immunohistochemical detecting tumor after treatment tumor cell apoptosis,
calculate the apoptosis index to compare the efficacy of treatment. Results:
In 43°C, with the increase of paclitaxel concentrations, are more obvious A549
lung cancer cell proliferation inhibition, the number of cells in living cells of
optical density value, the killing rate (cytotoxity index, CI). Cell apoptosis rate
increased. Heat treatment group the stagnation of the cell cycle in S phase, S
phase cells and G2 phase increases, S phase decreased in the chemotherapy
group, after heat treatment of lung cancer cells in electron microscope
magnetic apoptotic changes. The temperature inside the tumor can be
quickly rise to 43 °C. Tumors in three experimental groups are suppressed,
magnetic thermo-chemotherapy group tumor growth inhibition is more
obvious, immunohistochemical confirmed the tumor cell apoptosis in change,
apoptosis index increased. Conclusion: In vitro, with the increase of paclitaxel
concentrations, are more obvious A549 lung cancer cells proliferation
inhibition in 43 °C. The number of cells in living cells of optical density value,
the killing rate (cytotoxity index, CI), cell apoptosis rate increased. Thermochemotherapy
induced by nano-paclitaxel magnetic fluid can inhibit the
growth of A549 lung cancer nude mice transplantation tumor, nano paclitaxel
magnetic thermo-chemotherapy can enhance the anti-tumor effect in vivo.
Keywords: Thermo-chemotherapy, magnetic fluid, chemotherapy, lung cancer
POSTER SESSION 2 – P2.05: RADIOTHERAPY
MULTIMODALITY TREATMENT –
TUESDAY, DECEMBER 6, 2016
P2.05-028 COMPARISON OF ADJUVANT CHEMOTHERAPY WITH OR
WITHOUT RADIOTHERAPY IN NSCLC PATIENTS WITH STAGE IIIA-
SINGLE STATION N2
Jian Ni 1 , Yiqin Kuai 1 , Jianfang Xu 2 , Hongyu Wu 2 , Di Zheng 3
1 Department of Oncology, Shanghai Pulmonary Hospital,tongji University School
of Medicine, Shanghai/China, 2 Shanghai Pulmonary Hospital, Zhengmin Road/
China, 3 Medical Oncology, Shanghai Pulmonary Hospital, Shanghai/China
Background: The role of adjuvant radiotherapy (ART) on stage IIIA-N2 NSCLC
is still controversial especially in different status of lymph nodes invasion. We
conducted this retrospective study here to evaluate the effect of adjuvant
radiotherapy (ART) on non-small cell lung cancer (NSCLC) patients with
resectable stage IIIA--single station N2. Methods: Between January 2010
and December 2013, 383 resectable NSCLC patients with stage IIIA-single
station N2 were recruited in Shanghai Pulmonary Hospital. The patients
received neoadjuvant therapy or no adjuvant chemotherapy (ACT) and
those were mixed with small cell lung cancer components were excluded
from the study. Their clinicopathological data were collected and their
survival times were recorded. The last follow-up was finished on May 31,
2016. Kaplan-Meier survival method was used here to calculate the overall
survival (OS), disease-free survival (DFS) and Cox regression analysis was
used to conduct multivariate analysis. Results: Overall 341 patients with
median age of 59 yrs (25-79yrs) were included. There were 164 patients with
adenocarcinoma (AD), 106 with squamous cell lung cancer (SCC) and 61 others
(37 with adenosquamous, 26 with large cell carcinoma and 8 with sarcomatoid
carcinoma). Totally 26 patients were lost of follow-up. One hundred and
eighty-nine patients (55.4%) were confirmed recurrence and 152 patients
(44.6%) died until the last follow-up. Among them, 79 patients received ART
and ACT after operation and 262 patients only completed ACT. The patients’
baseline characteristics of these two groups were balanced. The median DFS
for the whole group patients, ART+ACT group and ACT group were 30 (23.9-
36.1), 31 (19.8-42.2) and 30 (21.8-34.2) months respectively. The median OS for
the whole group patients, ART+ACT group and ACT group were 52 (39.4-64.6),
54 (NR) and 50(36.7-63.3) months respectively. Multivariate analysis showed
no difference in DFS and OS between ART+ACT and ACT groups. In subgroup
analysis, we found the significant benefit in favor of ART (n=44) regarding
DFS (HR 0.55, 95% CI 0.323-0.938, p =0.028), and a tendency in OS (HR 0.553,
95% CI 0.284-1.078, p =0.082) in AD patients. While in SCC patients, ART
(n=18) seemed a poor prognostic factor. (HR 2.0 for DFS, 95% CI 0.935-2.485,
p = 0.074 and HR 0.757 for OS, 95% CI 0.225-2.553, p =0.654). Conclusion: ART
significantly decreased the risk of recurrence in Adenocarcinoma patients
with stage IIIA-single station N2 and might improve these patients’ survival.
The benefit of ART for SCC patients didn’t be proved here.
Keywords: Adenocarcinoma, NSCLC, adjuvant radiotherapy, stage IIIa single
station N2
POSTER SESSION 2 – P2.05: RADIOTHERAPY
MULTIMODALITY TREATMENT –
TUESDAY, DECEMBER 6, 2016
P2.05-029 MICROWAVE THERMAL THERAPY ENHANCES
RADIOSENSITIVITY OF HIGHLY INVASIVE HUMAN NON-SMALL CELL
LUNG CANCER H460 CELLS VIA INHIBITING DNA REPAIR
Yanyan Zhao 1 , Shirong Zhang 1 , Zhibing Wu 2 , Jingjing Zhang 1 , Lucheng Zhu 3 ,
Yasi Xu 1 , Shenglin Ma 3
1 Center for Translational Medicine, Hangzhou First People’s Hospital, Nanjing
Medical University, Hangzhou/China, 2 Hangzhou Cancer Hospital, Hangzhou/
China, 3 Department of Radiation Oncology, Hangzhou First People’s Hospital,
Nanjing Medical University, Hangzhou/China
Background: Hyperthermia has long been recognized as a modality in
anticancer therapy. In present study, we provide an update on the recent
knowledge about the molecular mechanisms of thermal radiosensitization on
highly invasive NSCLC cells. Methods: In previous study, we isolated invasive
subpopulations of cancer cells from established human non-small cell lung
cancer (NSCLC) H460 cell lines. The subpopulation of highly invasive NSCLC
cells (H460-INV) showed cancer cell stemness, increased DNA damage repair.
H460-INV cells were exposed to hyperthermia and irradiation. Cell survival
was determined by an in vitro clonogenic assay and growth curve for the cells
treated with or without hyperthermia. Immunohistochemical staining assay
was performed to detect the expression of Ki67、γH2AX foci. Cell apoptosis
was performed by Flow cytometry. Cell-scratches and transwell invasion
chamber experiments were performed to detect the ability of cell migration
and invasion. Western blot assay was used to detect DNA damage repair
related molecular changes. Results: Hyperthermia can significantly enhance
irradiation-killing cells. SER was 1.823. Ki67 immunofluorescence results
suggested that thermo-radiation can significantly inhibit cell proliferation
(p < 0.01). Flow cytometry results showed that the apoptotic cells increased
significantly in heat treatment group (p < 0.05). Compared with the control
group, H460-INV cell migration and invasion ability significantly reduced.
WB results suggested that thermal downregulated the expression of E
cadherin, upregulated N-cadherin. Relative persistence of γ-H2A.X nuclear
foci in the H460-INV cells after IR treatment was observed, when compared
to the no treat H460-INV cells. WB results suggested that thermal combined
with radiation inhibited the DNA repair by inhibiting expression of Ku70 and
Ku80. Conclusion: Microwave thermal therapy can increase the sensitivity
of highly invasive NSCLC cells to radiation and its mechanism may be related
to inhibition of radiation induced DNA damage repair, promoting tumor cell
apoptosis, and thermo- radiotherapy can inhibit tumor cell invasion ability.
This study suggests a beneficial clinical impact of maicrowave thermal
therapy as a radiosensertizer for benefiting highly invasive lung cancer
patients.
Keywords: hyperthermia, radiosensitivity, non small lung cancer, DNA damage
repair
POSTER SESSION 2 – P2.05: RADIOTHERAPY
MULTIMODALITY TREATMENT –
TUESDAY, DECEMBER 6, 2016
P2.05-030 WBRT PRIOR EGFR TKIS IS EFFECTIVE TREATMENT
OPTION FOR NSCLC PATIENTS WITH CNS METASTASES HARBORING
EGFR MUTATION
Pawel Krawczyk 1 , Marcin Nicoś 1 , Dariusz Kowalski 2 , Rodryg Ramlau 3 , Kinga
Winiarczyk 2 , Katarzyna Szyszka-Barth 3 , Katarzyna Reszka 4 , Kamila Wojas-
Copyright © 2016 by the International Association for the Study of Lung Cancer
S549