Journal Thoracic Oncology

Abstracts Journal of Thoracic Oncology • Volume 12 Issue S1 January 2017
targeting against lung adenocarcinoma with preserving immune function.
POSTER SESSION 3 – P3.01: BIOLOGY/PATHOLOGY
FUNCTIONAL BIOLOGY IN LUNG CANCER –
WEDNESDAY, DECEMBER 7, 2016
P3.01-041 ANTI-CANCER EFFECT OF HYPEROXIA ON HUMAN LUNG
CANCER CELLS THROUGH OXIDATIVE STRESS MEDIATED ERK
SIGNALING
Hyonsoo Joo, Jin Young Mo, In Kyoung Kim, Hyeon Hui Kang, Sang Haak Lee
Department of Internal Medicine, The Catholic University of Korea, Seoul/Korea,
Republic of
Background: Abnormal increases in reactive oxygen species (ROS) in cancer
cells serves as a target for tumor-selective killing. Also, several experimental
and clinical trials studied the effect of the hyperoxia condition by difference
of response between normal cells and cancer cells. In this study, the
hypothesis tested was that normobaric high oxygen concentration would
have anti-cancer effects such as inducing apoptosis on human lung cancer cell
line. Methods: Human bronchial epithelial cells (Beas-2b) and human alveolar
adenocarcinoma cells (A549) were exposed with hyperoxia condition in a
time-dependent manner. The changes in the cell morphology, viability and
protein expressions such as p53 and ERK were examined after the exposure of
hyperoxia (90% O 2
). In addition, to investigate whether hyperoxia condition
affects the production of ROS and cell cycle regulation, cells were analyzed
by a flow cytometry. Results: Exposure to the hyperoxia caused morphologic
changes such as atypical nuclei and numerous mitotic figures which inhibited
the cell viability in a time-dependent manner in A549 (p