Journal Thoracic Oncology

Abstracts Journal of Thoracic Oncology • Volume 12 Issue S1 January 2017
was measured by transwell assay and scratch assay; cell cycle and apoptosis
was examined by flow cytometry; the mRNA and protein expressions of VIM,
TWIST1 and CDH1 were detected by qPCR and western blotting, respectively.
Results: miR-30d was significantly downregulated not only in NCI-H2452
cells compared with MeT-5A cells, but also in chrysotile exposed MeT-5A
cells compared with the negative control and in plasm of asbestos exposed
subjects compared with unexposed subjects. After transfected by miR-
30d, the proliferation rate, migration rate and invasion cells of NCI-H2452
cells decreased, early apoptosis rate and total apoptosis rate increased in
miR-30d transfected NCI-H2452 cells. The mRNA and protein expression of
VIM and TWIST1 decreased and the mRNA expression of CDH1 increased by
overexpressed miR-30d in NCI-H2452.
(See Figures next page)
Conclusion: Downregulation of miR-30d is related to malignant mesothelioma
and asbestos exposure. miR-30d might be a tumor-suppressor miRNA, and its
down-regulation might contribute to pleural MM progression and asbestos
carcinogenesis.
Keywords: Mesothelioma, neoplasm invasiveness, microRNA-30d (miR-30d),
asbestos
POSTER SESSION 3 – P3.03: MESOTHELIOMA/THYMIC MALIGNANCIES/ESOPHAGEAL
CANCER/OTHER THORACIC
MESOTHELIOMA TRANSITIONAL –
WEDNESDAY, DECEMBER 7, 2016
P3.03-012 TUMOR-INFILTRATING LYMPHOCYTES, PDL-1, BAP-
1, VEGFR-2 AND IGF-1R EXPRESSION IN MALIGNANT PLEURAL
MESOTHELIOMA
Luca Ampollini 1 , Letizia Gnetti 2 , Matteo Goldoni 3 , Nicoletta Campanini 2 ,
Luigi Ventura 1 , Cesare Braggio 1 , Marcello Tiseo 4 , Valeria Balestra 1 , Paolo
Carbognani 1 , Antonio Mutti 3 , Enrico Maria Silini 2 , Michele Rusca 1
1 Department of Surgical Sciences, Thoracic Surgery, University Hospital of Parma,
Parma/Italy, 2 Pathological Anatomy and Histology, University Hospital of Parma,
Parma/Italy, 3 Department of Clinical and Experimental Medicine,university
Hospital of Parma, Parma/Italy, 4 Medical Oncology, University Hospital of Parma,
Parma/Italy
Background: to investigate whether there was any relationship between
survival and the expression of tumor infiltrating lymphocytes (TILs),
programmed cell-death-ligand-1 (PDL-1), BAP-1 (BRCA1-Associated Protein
1), VEGFR-2 (vascular endothelial growth factor receptor 2) and IGF-1R
(Insulin-Like Growth Factor 1 Receptor) in malignant pleural mesothelioma
(MPM). Methods: 63 cases of MPM were identified. All tissues were obtained
at the time of diagnosis. There were 40 males; mean age was 70.4 years. 34
patients were smokers and 40 had a certain history of asbestos exposure.
All histological slides were revised; there were 30 epithelioid subtypes, 20
biphasics and 13 sarcomatoids. The presence of TILs was scored as absent,
weak, moderate and strong according to a quantitative assessment on
hematoxylin and eosin slides. The expression of BAP-1, VEGFR-2, PDL-1 and IGF-
1R was analyzed by immunohistochemistry. The impact of asbestos exposure,
tobacco consumption and histological subtypes on survival were also
assessed. The survival analysis was analyzed by Kaplan Meier curve. Results:
TILs were present in 89% of cases and were found to be a favorable prognostic
factor (p=0.009) although related with histological subtypes (p=0.008). The
absence of TILs was higher in biphasic and sarcomatoid subtypes (90.9%,
30/33) compared to epithelioid MPM (53.3%, 16/30 p