Journal Thoracic Oncology

Abstracts Journal of Thoracic Oncology • Volume 12 Issue S1 January 2017
PUB094 CORRELATION OF CLINICOPATHOLOGIC FEATURES AND
LUNG SQUAMOUS CELL CARCINOMA SUBTYPES ACCORDING TO
THE 2015 WHO CLASSIFICATION
Yongfeng Yu, Shun Lu
Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai/China
Background: This study aimed to determine the relationship between
clinicopathologic features and lung squamous cell carcinoma subtypes
according to the 2015 WHO classification. Methods: We identified 368
operable lung squamous cell carcinoma patients who had undergone a
complete surgical resection at Shanghai Chest Hospital between April
2015 and March 2016. Results: Among all patients, the percentages of lung
squamous cell carcinoma subtypes were: 68.5%(252/368), 26.2%(97/368),
and 5.1%(19/368) for keratinizing squamous cell carcinoma(KSCC),
nonkeratinizing squamous cell carcinoma(NKSCC), and basaloid squamous
cell carcinoma(BSCC), respectively.There were more smokers in patients
with KSCC than in patients with other subtypes (p=0.004). There were
no significant relationships between pathological subtypes and other
clinicopathologic features, such as gender, age, location type, type of
resection, stage, visceral pleural involvement, lymphovascular invasion, and
lymph node involvement. The expressions of P40, CK5/6, TTF-1, Napsin A,
and CK7 were also not significantly different in three subtypes.Conclusion:
Our study revealed that there were no significant relationships between
clinicopathologic features and lung squamous cell carcinoma subtypes.
Keywords: Lung Squamous cell carcinoma, pathological subtype,
clinicopathologic feature
center of the lung by an air tube (bronchus). Large cell carcinomas can occur in
any part of the lung. Liposomes were used as Effective encapsulation of both
hydrophobic & hydrophilic molecules. Due to their ability to solubilize poorly
water soluble drugs, facilitate their nebulization, minimizing clinical drug
dose and reducing toxicity, prolonging and targeting release of therapeutic
agents by modification of liposome surface. Methods: The failures of the
present delivery systems are – Oral administration of etoposide at varying
dosage for the treatment of lung cancer causes GI toxicity and disturbances
of liver function. The parenteral administration of etoposide for lung
cancer treatment has been reported to cause severe hepatic impairment
and Hypotension. Moreover, intravenously injected ligands mediated
liposomes have been limited due to - Leakage of their contents before they
reach the target tissue, Rapid clearance from the blood stream, uptake by
the macrophages of liver and spleen. Results: Liposomes were prepared by
lipid cast film method, optimized and then coupled with Mannose. Drug
loaded Liposomes were characterized in-vitro for shape, size, and stability
in various body fluids. The Air-jet nebulizer system was used for aerosol and
was characterized for Appearance, Leak Test, Internal Pressure, Amount
discharge/ actuation, Spray Pattern area and Penetration Efficiency. The
in-vivo study comprised of estimation of serum and tissue distribution of
drug and fluorescence microscopy.Conclusion: Liposomes formed were
multilamellar and were found to be stable in gastric and intestinal fluids.
Fluorescence microscopy suggested that liposomes were taken up by the gut
associated lymphoid tissues and therapeutic level of drug can be achieved
at desired site. Thus, from the results obtained it can be concluded that
the Aerosolized mannose liposomes can deliver drug to the cancerous cells
in an effective way and hold great potential for lung targeting. Through
mannosylated liposomes, therapeutic level of drug can be achieved at desired
site. The system carried dual function, firstly retention of drug and secondly
selective delivery of etoposide to lungs.
Keywords: etoposide, Aerosols, liposomes
PUB095 COMPUTED TOMOGRAPHY IMAGING OF SUPERIOR VENA
CAVA INVOLVEMENT IN T4-CLASSED LUNG CANCER
Henda Neji 1 , Salma Jebri 1 , Monia Attia 1 , Ines Baccouche 1 , Mahdi Afrit 2 , Houda
Elbanna 1 , Soumaya Laabidi 2 , Saoussen Hantous 1 , Amel Mekkaoui 1 , Khaoula
Ben Miled-M’Rad 1
1 Abderrahmen Mami Hospital, Ariana/Tunisia, 2 Oncology, Abderrahmen Mami
Hospital, Ariana/Tunisia
Background: Superior vena cava (SVC) can be involved in many ways in
patients with lung cancer. This latter is considered to be the most frequent
cause of SVC syndrome which is a medical emergency requiring prompt
evaluation and treatment. The SVC syndrome can reveal the disease or can be
discovered on computed tomography (CT) of the chest. The objectives of our
study are to specify CT characteristics of SVC involvement in T4-classed lung
cancer and to estimate the frequency of its total obstruction in this patients
category. Methods: Retrospective review of CT scans of 177 consecutive
patients with T4-classed lung cancer, newly and fully diagnosed in our
institution during the year 2015. All CT examinations were performed after
contrast agent administration. Only patients with mediastinal involvement
were retained. Every aspect of SVC involvement was noticed separately.
Results: Among the 177 patients, mediastinal invasion was observed in 132
cases (74.5%). SVC was involved in 32 cases (24%). Lung cancer was located
on the right side in 91% of cases. The involvement included: - A contact with
the tumor with loss of the fat layer (n= 7) - An inclusion of the vessel in the
tumoral mass with narrowing of the lumen (n=17) - An intraluminal tumoral
bud (n=8) - A complete obliteration (n= 6) Collateral venous circulation was
observed in 8 cases. Adenocarcinoma and small cell lung cancer were the most
frequent histological types responsible for SVC involvement Clinical signs of
SVC syndrome were obvious in only 6 patientsConclusion: Superior vena cava
involvement is quite frequent in advanced stage of lung cancer. However,
the total tumoral obstruction of this vessel is rare. CT helps defining the
mechanism and the severity of the SVC involvement.
Keywords: superior vena vava, lung cancer, Advanced stage
PUB097 CARBON NANOTUBES FOR EFFICIENT MITOCHONDRIAL
TUMOR TARGETING
Gomed Agarwal 1 , Saurabh Bhargava 2 , Vishal Bhargava 1
1 Guru Teg Bahadur Hospital, Kanpur/India, 2 Manav Bharti University, Kanpur/India
Background: Cancer is uncontrollable growth of cells which are devoid of
apoptosis. We developed a novel strategy ie Ligand mediated tumor targeting
via carrier systems. Multiwalled Carbon nanotubes (MWCNTs) were used as it
directly enters into the cell without passing through endo-lysosomes, large
inner volume, distinct inner and outer surfaces & have ability to enter the cell
by spontaneous mechanism. Thus, proposed work envisages Rhodamine-123
conjugated Paclitaxel loaded functionalized-CNTs to provide enhanced cell
permeation in order to enhance mitochondrial availability of Paclitaxel.
Methods: The raw MWCNT were procured and purified, oxidized & then
conjugated with rhodamine-123 by carbodimide method. The MWCNT’s were
characterized in-vitro for shape & size by Scanning(SEM) & Transmission
Electron Microscopy(TEM), FTIR analysis, X-ray diffraction and zeta potential
determined. Stability studies were performed at exaggerated conditions
along with Hemolytic Toxicity Study. The Cell Cytotoxicity Study-MTT Assay
was done using Hela cell lines. Mitochondrial localization was determined
by CLSM study. The in-vivo part of the study comprised of determining the
distribution of drug in various organs by fluorescence microscopy. Results:
The Rhodamine-123 conjugated MWCNTs were prepared and characterized.
The CNTs showed high paclitaxel loading, sustained release, and excellent
biocompatibility as evident by in-vitro drug release and low hemolytic
toxicity. MTT assay against HeLa cell lines suggested the potential anticancer
activity of the developed system. CLSM study suggested that mitochondrial
specific localization of Rhodamine-123 conjugated MWCNTs in HeLa cells.
Conclusion: Thus, Rhodamine-123 conjugated Paclitaxel loaded f-CNTs system
have potential to provide an enhanced cell permeation and mitochondrial
localization for effective tumour chemotherapy.
Keywords: paclitaxel, CNT
PUB096 ENHANCED DELIVERY OF ANTICANCER DRUG TO THE
LUNGS VIA LIPOSOMAL AEROSOLS
Mani Agarwal 1 , Mani Bhargava 2 , Saurabh Bhargava 3
1 Manav Bharti University, Kanpur/India, 2 Icfai University, Kanpur/India, 3 Guru Teg
Bahadur Hospital, Kanpur/India
Background: Lung cancer tends to spread very early, and is a very life
threatening cancer and one of the most difficult cancers to treat. Non-small
cell lung cancer (NSCLC) usually grows and spreads more slowly than small cell
lung cancer. There are three forms of NSCLC: Adenocarcinomas are found in
an outer area of the lung. Squamous cell carcinomas are usually found in the
PUB098 PANIC DISORDER AND DYSPNOEA IN PATIENTS WITH
LUNG CANCER IN A NIGERIAN DE,-ADDICTION UNIT
Victor Lasebikan
Psychiatry, College of Medicine, University of Ibadan, Ibadan/Nigeria
Background: The objectives of this study were to determine the prevalence
and dyspnoea and panic disorder in patients with lung cancer in a teaching
hospital and also do determine the association between them. Methods:
S792 Journal of Thoracic Oncology • Volume 12 Issue S1 January 2017