HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

24-48hK Absene ofhdctialgo*lh indicaled sterility of$e supern.rrnl Pr.$nceolHPS
virus wls detqoin.d by .xmininS allMtoic lluid dd livd lioo .nb.yos, uins ACP r.sr
ft€ mSV wB inml{.d to .nibtyos 6r ditr @t .gc ol ECE (8,12 d!y,old) via
CAM roure Th. b.sl vids lilte Dd opdml fluids woe ob$incd by imdlaing HPS vitus ar
lldit.oldEr^E t\ h6n large amou ol\'as(l\lO lDdO I nU $binocuht.d otrro. AM
of I l-dcy-old ECE, llPS vitus produocd rhick.nina of $. CAM, dw.rfisd, ha.mod4a,
r.d hcldnis Th. inoshrion ol.mbryo! wnh . brsc lDounr of HIS vim r-? d.ys ,{t r
inoculariotr Whetr 8-ddy-old ECE were ir@ulaled vl|h r (l x lor lodo I 'nl) kilt.d 'cm
bctw*n3-Tdaysposl inoculetion when 3-day-old ECE w. inocullr.d wnh. b€clnrunt
of vitur (l x lor IDe/o I nl) vi. YS, it Lill.d lh. .mhtyos wnhjn l-6 day3 followins
in@l*ion. Only 2 to 5 cobryG w. tdll.d, vh.n . smlll .nd ofvjN(lot'IDer'oI hl)
w.s administered via YS ud CAM, rcspcctiv.ly
Th€ rate of.nbryo infedivny by IIPSV ielat , w.s quit. hiBh when th€ virus were
propi8.r.d dd ririir.d via rhc Ys rour.. Thc it6 uq. v.ry hiBh (10' EID;ml) olalmon
lll Ys-Dropd!,lcd llPsv r$l0r.r lto*vcr, lhe CAM ri..r rrneed lion. lolo to lo'
ElD,/nl No siSnili clnt viru s lnr€ was d e@lahle u pon inoculddon through allanloic src, and
Rs|B oath. pr&d study !c in.8tmdt th thcelr olYard dd F.y (1e14
Winrrfi.ld sr d/ (1971) md Fidly (1975) who r.pon.d lhrt ldendiruss cdld .€dily
prop.Eare in clicko embryos Howev.r, Mcrermr {1980) rcponed rhal rhe pimary cell
cultures (CEK and CEL) may be nor. s.nsitive to infectio. lnd ad.novins prcPasation
Whcrc embryG... ui.d, inmhrion br rhc chonoaldtoic $c (cAs) dr. rc$rB in qune
g@d rirr6 of vitur, wnich wNld 6na. b.rss lo..lo ELDI wilh difr.Mr ishls The
lngh !n0s yjeld has b..n oblain d i'r .nbryos rcl oontiining n.utali2ing antibodies to lhe
nomolosous adcnovitu3 sor)"es Mor@v.r, €rn6ryd from bradins nock immu. to some
nomologdus adcnovitut s.r6t}?6 do @r rllo* vim dultipliotion (Fadly .id Winleridd,
l9?l; Frdly and winrdticld, I97l: Frdlr, I9?5).