HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

SUMMARY
llydropericarditrm lrndr6oe (HPS) cn a!i!n rde.ovitu ina€ction h.r bcen idenlitied
in poolt.y 0@ksallos nakistin This Foj.ct Ms dBisred to studyvrnoussp€crsoaHPs
in remr of ns Neliolog} (vitu i$laton, pu.in6tion, ind ptopaBaion t, p,/r.r, rouie ol
tansnbson, clinical picture, ud prd$logy In addition, idcnlity oa rh. conla8ium wst
conlim.d lhroueh nnmononuor.sne, .lcto. nicrospt biologial riklion, s.rorypinA
and nol*ulrr ch&cteiatio.. Andp8 wer. als rud. ro d4€lop improwd vaaiB
Inv.sriSllion on puined H?S oscnt indic.ted rhNt ili! isol,b wa5 ! nw avian
rddolins (AAU patholyp. b.longing ro sotyp€4 ol grouDr This kol.l. wm nm.d
ans P.kiian Asnahunl R.s@ch Courcil-l a PARC-I i$| 6 Tb. t.shs oaoneway
vitus ncurraliarion test with referenc. AAV antissa (l.ll) coinmed nrr n'e isolate
designrted as I'ARC-r ishre belonsed ro s.rorype 4. The AAV serotype4 kolarc has th.
potoriil to produ@ rh. imunop.gipnaing lnd virus ndtftlitng tntibodi.s
h wis obsew€d lhat this i$hl. is capable ol au3ing HIS in hroiler chicks. Th.
lesionsc.uled hyihisviruswereidenlicoltollr*ofHPS obs.fl.d underthctield oulbraks
ln emb.yonited chickd .98i, rh. i$lrtc ous monalily, g.n olized mulol.r @ngdion,
hep'riti! lnd su.r.d mbryonic BrNh Thir vios al$ ou!c! ir?i6l +oparhic .tr4c:
rounding, nDderale swelling and grapclike clundring upon inocolanon onro chicken embryo
liver (CEl,) celh The biological ch.tui.rizrion indidl.d thlr th. i$lare pARC-l pos4i.d
standnrd prop.di6 of oder Lnow sotyps of AAV. AllhouSl', rhc ncw isolde is
biolosiqlly ..d srologic.lly iddi@l ro *rotype4 ofAAV, ir is nore vidl.nr rhm th.
previowly hmwr strains oaerolype-4 TIE presd wor* hA turlhe. indic{.d rMt ielares
obr.in.n dof ditrGMr HPS @lb..ik ots th.ld l0 y.r6 h.v. iddric.l i, vrra ald t'
The polypeptide analysis usins SDS-PACE confrrmed the identiry of tlPS lirus
AAv t he protein patrcr. of p.ototyDc tlnir of serolype4 is qune conl'd.bl. with thN