HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...
HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...
HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...
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El.ctrophoreb vs 6icd olt chhc. i. Mighry Snatl cel TM [ (HoeGr scienlific<br />
InrrdrunB, Sd Ftuds, Califomia, UsA) or M.dim li4 c.l (sisq Cimish sr<br />
hui4 USA) Th. @pLs w .larophoi!.d it 10 'C !jn& 2t mitti eps.! (mAMlS)<br />
(Pwq Supply PS-lroO. Eef.r Scimdic Innruncm, Srn Frdcjs,<br />
Cdiaoflid, USA). Wha d. tBcting dye rcich€d lo the seplr ing gd n was ton at It<br />
nAM?S u il $e bronophdol blue dtc fiont cscap.d ftom the lowd .d8e of rhe Bel Thc<br />
optimum lmounl of splddd wrs d.t.rmi.ed by Eparen dak<br />
Th. polyFptide bmds wG dd6r.d by tttning rhe g.k virh C@oaicbnlior blu.<br />
(R-250) disolvcd i! 25% nElhqol loy. gl.cirl &cric lcid &d distilld Mld Tn gelr wcrc<br />
sci..d for I min in microMvc dci (N!don.l, ,ap.n) ai loe pow.. .oil dc$aj.ed wilh .<br />
$lulion @nsisting of25% nelhmo! ind ICr'l. glacial aeio aoid, for I min in micro*ave 0l<br />
low pows Th. gel wm th€n lrmier.d ov.r a 6lter pap€r dd died for tvo nouF ar 30 "C<br />
using a slab-drycr (Model443, Bio-Rad Lrbodloris, Richnoid. Cllifonj., USA), hok.d<br />
to ! ncg.livc p.eurc t@uo surc. (Gat MiuftdurinS c.rpodion, B. on H.rnor,<br />
MicliSd, USA). Th. gcl. w phorogntlr.d !i.A lo.odrft Poldoid w!.
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