HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...
HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...
HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...
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Rdlts ofrhis srudy ee ongodt wirh rh. findi.as ofwim.rfield.r d/ (197r) who<br />
reponed thtl sooe hddovitus holalcs, $rch as dt <strong>IN</strong>D-C virus, have been propagated<br />
suc.stully by rh. YS rnd CIS .out6 ofi.oolaion; ornd grow mrc @dilr rhrough YS<br />
rourd of inocul.tion FindDgr of Fadly lnd Winlclll.ld (19?l), and Fadly (1975) suB8c$<br />
wi.iioN imona i$l0t.s of ad.mvitur from chjcl(cB in lh.n .biliry b mukiply i. chido<br />
embryos. Fdcro6 thit .lf.ct th. HISV in protagaion ire .'nbryo 3cetribilfy, age of<br />
embrros, and roure of in@lat io., rnd andbody rlol us of bE dq Rocrs ti€lding ECE<br />
The0ndin8soath.presentsrudyarein@nfooityvilhlheobs.N ionsofYit$and<br />
Fry ( 1957), Glllina ./ o/. ( l9?3) and wincrfi.ld e/ a/ { l97l) who @rd.d lhal i$l.tcs ftom<br />
chiok.ns oncn cause dclth of eEbryos in 2-7 days upon inoctrlarion vit lhe CAS route lr<br />
drlt dqth o@rn onbryG indi€r. gd.dl msoltr 6n8.slior or hicn$dhagic lgioos<br />
Delay in desrh, slunling and drling is rho obs.d vhq eobryos d. ini4led *ith avitn<br />
inferioue bro@hiris vitut. Minur. op.qu. plrqus o. thc CAM ..c $m.tind 5n Eobryos<br />
onen posess helatitis chtrmteiad by varyi.g degr@s of moltling and Ectols<br />
Hisrologielly, inur.trclar i.clujon hodq (lNlB) tG pr.snl h hePllocvr.s Inll.nrorory<br />
ccll infihmte in lhc Donal are8 ol liver rcdpoed olheterophik and liuroblastic cslls are<br />
obl.fud b, Gallina./4t (197r).<br />
F0dly.nd wint.rield (19?l) and Fadly (1975) tepolted rhai rhe €mhrto dods<br />
follo,ins YS i@dlarion of Tiplon $rain vjds sasllly @r wjlhin 5.l 0 d0's Pl Th.<strong>IN</strong>|B<br />
dc $cn in h.p.tocyG of.nbryosrhot dicslare d! 10 day3PI lfthclirusk inocul.led on<br />
rh. CAM il lcday old.mbryc, oDaqu., ncrotic phqns ir. evidenl. lt is qun. sisninanl<br />
1har olly tlE cmbryos not containinB specilic virus neuralizing {VN) a.tibodies and<br />
mployng rh. YS 'outc lor $4stu1 ptopiSt'ion of thB stu! $ain<br />
Cell Culture Inoculrtion:<br />
P.tnobgy ofi et d cdr.drlrd<br />
Udinfeded ohi.ken enbryo liver (CEL) c.llE tormed larse irresuhr shapsd isllnds or<br />
.pnhelial cells wirn a Gw tbroblsllikc eldngrio$ i' lhc apa@s bctwed th'm The<br />
evenruallyjoinedlosetherasth.y8rryoutIoform!w.ll_delin<br />
d, u.'ionn monolavd wnh|n
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