HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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T.hL lV-4. Virur n.uhlttiio. suhi Sr lr.lrtJ ri..lbtr NornlL.aion tllF $th AAV rtit.n I | 23a56?1910 ll l HPsv. lbd 2HPSVbMd--->8 lmsvcLhr--->3 i: All i$brs w ft6 mrhd liwr, groprgltcd ep'r 'lvfdtrptorh@ p.ssagd in CEL ccll ottut. .r Tir6 e api:3!.d .t $. diproc.l of th. high.sr dilDtion of mm qusins @dtr. Mtnli4rion ot 50 b too mcdtrn 1is. otuc inf4d@ dold (TCIDT) ofvitus EPSV: Hydr6pdiwdium .rndrom. virus .-ltdr hlmlh.d di&icr xd $mundi.s d{ b=Mrni Mdehr. dhlri.t.nd sutrolldinB a@ F!!r blroc didticr .nd Dfloundi.A .@ d=Frd: F.isalabad dislriot and ercundDg .t is eK.r: Kmchi dn|.d md $roundins a4
ourbreaks oaHPS e.rlicr..porrcd in lil€dtu.e (Ch@o. e, al, 1989r Anju6 ., a/, 1939i AMul,Azia lnd H.sn, 1995; Covo ur zl, 1996) Th. pdhq.niciry ofrh. i,ol.re w6 lho lppdedt fiom tbe mon.liy of.nbryos,lsions on thc ebbryos 8nd cylopothic.trect (CPE) produc.d in chictd .mbDo liv.r (CEL) c.lk. Th. findinB ofthh sudy indicate lhit rhe AAV isolate PARc_l Po$.s all the mqjo! physicd and ch€mial p.op€di6 oarde.ovi$ such s th. prenc ol6s. irl lipi&, nucleic icid iypc, lhermal elibihy, tesistance lo qtrene pH ind sneofr.Plication (McFeran,1939) Thb indiorB thar tn. tw irol.t. bclongr lo !{rrp.4 of AAV h.ving oh.nc.d vitul.E rh.n odliq known $raift ofsblyp.-4. tt hd bc.n.irlid.nrblithcd in cr@ of inclurion body h.pltitis (IBH), where vituloce h4 hen d.mnstnted b.twd i$ld6 of lhc em. lnd ditr rdt serolypcs ofAAV (Wi efield,1934rCow.n.t4i l9t6) ln rlE prBcnl ,tndy oFw.y neumlianion t.sts w. petfom'd To tunh6 invesriSar. soolotsicd tedtidnships beN.cn isola&s n t @.$!ry ro cirry 6ur rwo_w'v n.utraliation iesls P.ovious studies have indidted lh.t idenoviru3 isolal4 po$e$ t bto'd sp.clrum of.ntieenicil, ..d miy do$ r.rcl wirh morc lht' on' stvp' ll is ih'cforc lugsdlcd thar borh r6riction .nzyne iRE) ant$h lnd tulk iaton r'i3 with r'f'Ence .ntisa nor. rhln on. sotyp. .r. requn d ro tullv ch'ncreri& tddovitus i'soltts rs a dos genomic relarionship do6 not ndsilv inplv '3e'olosicll relar'dne$ (Gough dnd
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OBSERVATIONS ON CAUSATIVD AGENT(S)
Page 3 and 4:
"M sooD&{!B N|,lr97
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,/2 ./. -t4 In tlrc tnme of God. ll
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ACKNOIYLEDGEMENTS "Pro(hrm in th. n
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AC(NOWLEDGEMENTE LIST O[ TABLES LIS
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Cell cuhur. inocul io. PriMry viNr
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5. RJSULTS AND DISCUSSION coNclUsto
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EXPERfMENT TNL VIll S€tuo aitibod
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T N TRoDUc7IoN
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nds in 0ocks is th€ onty signilio
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R E v T E w o F L t T E R A T U R E
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highly d.trs I'otlrry ar@s in the i
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A l.orFdc Poi$n.: h) Chlorin.red hy
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Oc@ny (Vop, | 989) Tl'.ic qinr 6nro
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pig or rit. IndnEr ha.m.gsluri.0rio
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Mrner g, z/ (1989) iiocutded lh. su
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virilotigin, perhaps an adenovirurw
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(AGPTT H.sn dr dl, t994) whit. d.re
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Trbl. l.t S.npd.l.!e or Ad.ldids Nu
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Th.s vinoN ae rcid sllblc (Hamparia
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Tte viruss sharc a 6mnon goup antis
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Trbl. Lar S.relogi..l .l.ici6..tio.
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dnibodi., are rising in lield cas6o
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nun'ler ot polypenrid. bands derib.
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Phosphokinls. ocrivity lssi.lcd wil
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sigm. Th.dis4e, whichcanbeqpe mcn$l
Page 54 and 55:
nrrenar oibody vitu! wc i$hr.d fron
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Th. oain problcm with any sologidl
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obwed ihar AAv-polypaptidg could b.
Page 60 and 61:
M A T E R I A L s A N D M E T H o D
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lrydld.tot tc.: Th. $dlr vq!.odn d
Page 64 and 65:
Infation ofchi.k Th. experim.nht ch
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lhe monolaytr (3-5 diF), ihc 06ks w
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..diftg.d d 90,00 X Sb. 2. hor A ti
Page 70 and 71:
Trta |lLr. lr..b.d.t D.Ln h. r.*. .
Page 72 and 73: EXPLR]MF.NT.IV BIOI,OCICAL CHAMCTOR
Page 74 and 75: ^\]\ M.di.r tgg Inleriv. Doe (EIDq)
Page 76 and 77: nin Th.r. cells ud. slored.t -20'C
Page 78 and 79: EXPI;RIMLNT V CHARACTI]RIZATION OF
Page 80 and 81: El.ctrophoreb vs 6icd olt chhc. i.
Page 82 and 83: @ ainjr8 l./r gchriq dd inob.t.d fo
Page 84 and 85: ofPSS .nd srored at -70oC. Pr.&nce
Page 86 and 87: T.bb V[.], Etpdindtd d6ltr ofv.ccbr
Page 88 and 89: I;XPERIMENI.VlII A DOT IMMUNOBINDIN
Page 90 and 91: R EIUL7I A No o Isc u saIo N
Page 92 and 93: lrig.l, ll.Nrtor!chiclcn dl.d (72 l
Page 94 and 95: Fi8.3, Int.m.l or8.r of (hi.kdr,dl.
Page 96 and 97: ig.5. 8!6rotl.rbri.nBlronHPSig.ntin
Page 98 and 99: Fig. ?. Photodlcrcghpt! of h.rrr rl
Page 100 and 101: f.bL l-t. In nw ,a.l In utuo Ch.r..
Page 102 and 103: 24-48hK Absene ofhdctialgo*lh indic
Page 104 and 105: Rdlts ofrhis srudy ee ongodt wirh r
Page 106 and 107: lndlr.d nuorcsc.trl rntibody (IFA)
Page 108 and 109: PURIFICATION OFTIIE HPS VIRUS men.e
Page 110 and 111: da'dy of l.3s tnto|f i' c|O. noF€
Page 112 and 113: T.bL tl!l. Mo.t lit h DFiL. cti.t .
Page 114 and 115: Pithogmic in rni! nudy lhould ody b
Page 116 and 117: T.bl. w-r. Ell6t ol C.tl Cutrrn psh
Page 118 and 119: The anrier., ftis.d agrinsl purifi.
Page 120 and 121: T.bb rv.3. fttldl .rd Cr.r&.t Cr.st
Page 124 and 125: EXPE]IIMLNI.V CII RACTERIZATTON OF
Page 126 and 127: SDSPACE shorira con0uison oa l l *r
Page 128 and 129: Fis -Va rlso indiqt. that in &mtyp
Page 130 and 131: a ibodt in 6 erun of low iite. The
Page 132 and 133: Thc resulls ofSDS-PAOE ds re{@Gd ll
Page 134 and 135: Trbl. Vll-|. S.tun a!.ibodid rg.inr
Page 136 and 137: s 225 175 125 tm 25 :i--.'' i,; \\
Page 138 and 139: T.hl. vl|,3. 0oir.hrllcng. Mort.lly
Page 140 and 141: Tlblc Ml,4 indic.t6 lhd lhc pdort?c
Page 142 and 143: I chdt.ng. t.ot.r.. Fls.Vll,3. trcr
Page 144 and 145: 'l rbli Vlll. l. Scnrilivily ThBrho
Page 146 and 147: Tabh. VIII. 2. S.nlitivhy threlltol
Page 148 and 149: T.blq Vlll. a. D.lalion oa vinl .nt
Page 150 and 151: n g.tive re;ons Althoush a dositomd
Page 152 and 153: SUMMARY llydropericarditrm lrndr6oe
Page 154 and 155: c o N c L U s I o N s
Page 156 and 157: R E c o n M E N D A 7 I o t I
Page 158 and 159: L T 7 E R A 7URE c I7ED
Page 160 and 161: Arhr,s,s z idulMI xt'r ' lrez. Risr
Page 162 and 163: Cool.lK.A l9t2 Avi,nl|.miGiLturbnor
Page 164 and 165: Gim,TM,DJ Kil!andsH xkva le?6 ^prli
Page 166 and 167: Mirtrtrood, F, RU. Sh,r,id, Mz Ktun
Page 168 and 169: ftrcifl, H 6 1156 n?ii! of ldctuida
Page 170: v.D Ecr, J.H H . ll.C DiErsr, rA M
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HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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