HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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obwed ihar AAv-polypaptidg could b. lnalyDd by SDS-PAGE usi.g th. blfe systcd of liemdli ( I 9?0) Li €r al ( I 934a) w.s cbl. r o distinSuidr ar ldst t 4 ittocrural Dotypeptid6 in Scls aollowin8 SDS PAGE. Th. poly?epridcs ar. visurriz.d a&e. srainine wiih coom!$ie brillia blue sbins Thc MW oa stociural polr?epdd4 @iB fron 5 KD ro l2o KD (Nonby et a] , 1t16,\liBnd et al., 1932) 8nd lor FAv, 6 KD ro 100 KD (Li r/ aa, 1984a). This tech.ique is prindily uql lor deteing otibodid to individu.l viril p.ot inc od for d.fining sp*ifi.iry ol mn@lo@l a ibodi6. Tnc leh.iquc h0s ldvdr.g.s ovs r.di6- immunopr€ipitatio. in th ialh. inlisd is pDvidcd i. dcd, .h. anribody bjnding 6ry be quantifi.d Th€ nnro@llulos. mcmbrdca firve b..n used to 'elain protcins fron dilule solulioB lor th.i. eb$quml qu..uratv. ddemin ro.. Towbin g, al (19?9) d€cdb€d varioue sr.ps such as eleclnphoretic blottin& ndning lnd immurolocicdl deteotion of prol€iN requncd fot rhc mrLr oa prot.iN from lhc polyrc.ylmid. g.l {o . nitr@llulos shel They ob&R.d rh.i p.otein! i.Dobilizcd on a dtr@llulose lhcet cotld be u!.d ro ddd lhor rcaporiv. inlibodi.s th. blofiing t4hniqu. hds thc Potqlirl for immuno.leclrophordic .n.lysis oi pror.ins by nsing bindins of lah frlSoents ot bindin8 oa r.ihodier againn r sin8j. d.l.mi..nl such ts oonocloml anlibodi6 Studi* h!v. shown th.l 66p0r.n ro lhc .l@lbpho.ctc t@sfer or prorcinc ro diizobcnzyloarmolnyl (DBIO prp.r, w.ddn blolting with ulmodificd nirroc.llulo$ 8iv.s nrkedly b€ncr re$lntion, i! 3i8nifiqn y 6i.r to pdlom dd d66 @t €qun pre_ equilibrstiod olgrl nor diirclialion oflhc nnrocellulose (Suoette, I93l) w.sldn bbnra knniqu. h6 bcn ued fot.h@laianion of imu.o8o3 olad€novirus (hn dcn Huik dd vm Dtuno Liul.-vor d.n Hurk, 1988) Propiyl.th rnd conrml Sie ddr onv. ioml ldenovitus s4ottte hav. nor bcn associ.t.d vith welr d.ffncd clinicil sy.dromq 6 rol me$ue hiv. nol b.cn dablbhcd For vaeimlio. lgain3| IBH, Fldly ed wi dn€U ( 1975) uscd rhc Tipron i$htc pssaed ?,1 lic in chid cmbryos Th€y d.momtrded th.l va@instcd chioks w4c moch l4 ssoeptible lo
tubdtDou clBlLngc fie mn-vui.rt d Itrd!. Fddondq $bot.||@r vgidtioD ioduc.d highd neui..[atioi indiq thin .re drop vlcciMnon, Gd@ !d King (19??) tunhd demo.srar.d thal birdr {irh . !r.l dtibody to FAv-8 w... morc r6isri.r to honolos@r infdio. rh.n th. bir& vidsur dilFdy SiE IBH ha hd' Mi.r.d with DAiy ld.novios! (McC@k n .t al, 19161cov.n et al., t ), .nr iiturc IBH vroirc volld pr.$n.bly hrve b in@Aonte sqd solyp.' Ho*t€, undl lhe .cliolo9 .nd D.lhog@* of BH bE tcn ddfi.d th. dllG .rd .ltc.cy of ||hwiru3 vdin6 oMr By ir! nrtur..fsddd oni.l md thon ihr|lion wittir . f,et lrearm.nt of IBH h g*nlly @irha r.quircd m. e{fctiE. Ir hs b4n obs.d Ihn IBDV i eri6n di.r'6 the prhos.ni.ity ol lBHr nslur.l .nd qp.dmalll sar would iididc thar itf.ctron eitn IBDV h mt n*$riy for o@rcmc of IBH (M.rcru, l93lh). Ttus, tEinition !9insr IBDV dy rct n...gily .lf4t the dE of IBH Psibt otl'd inmm$ppBiE asdt!, sch rr clict !m.mi! lgent obi@ lhe Ptthoaoi.ily ofldercvitu$ in IBH (Ot|!i cral. 1933: CoM.rdr., t996)
Page 1 and 2:
OBSERVATIONS ON CAUSATIVD AGENT(S)
Page 3 and 4:
"M sooD&{!B N|,lr97
Page 5:
,/2 ./. -t4 In tlrc tnme of God. ll
Page 8 and 9: ACKNOIYLEDGEMENTS "Pro(hrm in th. n
Page 10 and 11: AC(NOWLEDGEMENTE LIST O[ TABLES LIS
Page 12 and 13: Cell cuhur. inocul io. PriMry viNr
Page 14 and 15: 5. RJSULTS AND DISCUSSION coNclUsto
Page 16 and 17: EXPERfMENT TNL VIll S€tuo aitibod
Page 18 and 19: T N TRoDUc7IoN
Page 20 and 21: nds in 0ocks is th€ onty signilio
Page 22 and 23: R E v T E w o F L t T E R A T U R E
Page 24 and 25: highly d.trs I'otlrry ar@s in the i
Page 26 and 27: A l.orFdc Poi$n.: h) Chlorin.red hy
Page 28 and 29: Oc@ny (Vop, | 989) Tl'.ic qinr 6nro
Page 30 and 31: pig or rit. IndnEr ha.m.gsluri.0rio
Page 32 and 33: Mrner g, z/ (1989) iiocutded lh. su
Page 34 and 35: virilotigin, perhaps an adenovirurw
Page 36 and 37: (AGPTT H.sn dr dl, t994) whit. d.re
Page 38 and 39: Trbl. l.t S.npd.l.!e or Ad.ldids Nu
Page 40 and 41: Th.s vinoN ae rcid sllblc (Hamparia
Page 42 and 43: Tte viruss sharc a 6mnon goup antis
Page 44 and 45: Trbl. Lar S.relogi..l .l.ici6..tio.
Page 46 and 47: dnibodi., are rising in lield cas6o
Page 48 and 49: nun'ler ot polypenrid. bands derib.
Page 50 and 51: Phosphokinls. ocrivity lssi.lcd wil
Page 52 and 53: sigm. Th.dis4e, whichcanbeqpe mcn$l
Page 54 and 55: nrrenar oibody vitu! wc i$hr.d fron
Page 56 and 57: Th. oain problcm with any sologidl
Page 60 and 61: M A T E R I A L s A N D M E T H o D
Page 62 and 63: lrydld.tot tc.: Th. $dlr vq!.odn d
Page 64 and 65: Infation ofchi.k Th. experim.nht ch
Page 66 and 67: lhe monolaytr (3-5 diF), ihc 06ks w
Page 68 and 69: ..diftg.d d 90,00 X Sb. 2. hor A ti
Page 70 and 71: Trta |lLr. lr..b.d.t D.Ln h. r.*. .
Page 72 and 73: EXPLR]MF.NT.IV BIOI,OCICAL CHAMCTOR
Page 74 and 75: ^\]\ M.di.r tgg Inleriv. Doe (EIDq)
Page 76 and 77: nin Th.r. cells ud. slored.t -20'C
Page 78 and 79: EXPI;RIMLNT V CHARACTI]RIZATION OF
Page 80 and 81: El.ctrophoreb vs 6icd olt chhc. i.
Page 82 and 83: @ ainjr8 l./r gchriq dd inob.t.d fo
Page 84 and 85: ofPSS .nd srored at -70oC. Pr.&nce
Page 86 and 87: T.bb V[.], Etpdindtd d6ltr ofv.ccbr
Page 88 and 89: I;XPERIMENI.VlII A DOT IMMUNOBINDIN
Page 90 and 91: R EIUL7I A No o Isc u saIo N
Page 92 and 93: lrig.l, ll.Nrtor!chiclcn dl.d (72 l
Page 94 and 95: Fi8.3, Int.m.l or8.r of (hi.kdr,dl.
Page 96 and 97: ig.5. 8!6rotl.rbri.nBlronHPSig.ntin
Page 98 and 99: Fig. ?. Photodlcrcghpt! of h.rrr rl
Page 100 and 101: f.bL l-t. In nw ,a.l In utuo Ch.r..
Page 102 and 103: 24-48hK Absene ofhdctialgo*lh indic
Page 104 and 105: Rdlts ofrhis srudy ee ongodt wirh r
Page 106 and 107: lndlr.d nuorcsc.trl rntibody (IFA)
Page 108 and 109:
PURIFICATION OFTIIE HPS VIRUS men.e
Page 110 and 111:
da'dy of l.3s tnto|f i' c|O. noF€
Page 112 and 113:
T.bL tl!l. Mo.t lit h DFiL. cti.t .
Page 114 and 115:
Pithogmic in rni! nudy lhould ody b
Page 116 and 117:
T.bl. w-r. Ell6t ol C.tl Cutrrn psh
Page 118 and 119:
The anrier., ftis.d agrinsl purifi.
Page 120 and 121:
T.bb rv.3. fttldl .rd Cr.r&.t Cr.st
Page 122 and 123:
T.hL lV-4. Virur n.uhlttiio. suhi S
Page 124 and 125:
EXPE]IIMLNI.V CII RACTERIZATTON OF
Page 126 and 127:
SDSPACE shorira con0uison oa l l *r
Page 128 and 129:
Fis -Va rlso indiqt. that in &mtyp
Page 130 and 131:
a ibodt in 6 erun of low iite. The
Page 132 and 133:
Thc resulls ofSDS-PAOE ds re{@Gd ll
Page 134 and 135:
Trbl. Vll-|. S.tun a!.ibodid rg.inr
Page 136 and 137:
s 225 175 125 tm 25 :i--.'' i,; \\
Page 138 and 139:
T.hl. vl|,3. 0oir.hrllcng. Mort.lly
Page 140 and 141:
Tlblc Ml,4 indic.t6 lhd lhc pdort?c
Page 142 and 143:
I chdt.ng. t.ot.r.. Fls.Vll,3. trcr
Page 144 and 145:
'l rbli Vlll. l. Scnrilivily ThBrho
Page 146 and 147:
Tabh. VIII. 2. S.nlitivhy threlltol
Page 148 and 149:
T.blq Vlll. a. D.lalion oa vinl .nt
Page 150 and 151:
n g.tive re;ons Althoush a dositomd
Page 152 and 153:
SUMMARY llydropericarditrm lrndr6oe
Page 154 and 155:
c o N c L U s I o N s
Page 156 and 157:
R E c o n M E N D A 7 I o t I
Page 158 and 159:
L T 7 E R A 7URE c I7ED
Page 160 and 161:
Arhr,s,s z idulMI xt'r ' lrez. Risr
Page 162 and 163:
Cool.lK.A l9t2 Avi,nl|.miGiLturbnor
Page 164 and 165:
Gim,TM,DJ Kil!andsH xkva le?6 ^prli
Page 166 and 167:
Mirtrtrood, F, RU. Sh,r,id, Mz Ktun
Page 168 and 169:
ftrcifl, H 6 1156 n?ii! of ldctuida
Page 170:
v.D Ecr, J.H H . ll.C DiErsr, rA M
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HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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