HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

sanples ofvirus dnd CEL conrlolarrigen w.!c dotted on each pumlvrlrip at I cm apan and
wcE allowd to dry ar rmm t.mpeatnr. Un@ted prot.ir-bindinS sir6 q6. btocked by
immssing and tocldns th. stiiF in I % 8.hti.
in Tris-butr r slinc (TBS: 20 mM-Triq 500
mM-NaCl pH ? 5) lor 90 min, folloved by vashing in plain TBS for 5 nin The nitro@lllos.
slrips wqe enhc. u$d ituEdi cly or dti.d md storcd 4 'C fo. tuluc u$.
T[e nnocdllulose srips were pla*d in re$ sen dilured l]20 in TBs lor 90 min ar
.@m !.dperaruc on ! sh'lcr Bort" .hc lnom polirivc lnd n.Sdivc s. @nkols xr.
included ro dereffiinc lhelp@iicilyofrheDlA Ai€r wlshi.g 4 tim.s in TBS aSnalin8 lor 5
min in rh. la$ wrnr, llE slrip w.s uhroged in nbbil lnli-chiclq lsc @njusal.d wirh hor*
Bdish t roxide dilur.n b |:I,000inTBs
And 90 nin of imbitio. i. room l.operalur. lh. stipr w.r. vashcd .s.j! in lh.
mnner dsnb.d rbov. dd vcrc dcvcloped in 50 nM TBS ont.ining 015%
diminobenzidine, O 04% nickelchloride (NiCl:), and 002% hydogcn p4oxid. {H,0,) Th.
rog.nre we.c al,ov.d ro rdct for 25 s@nd' Tne dn!! we vah.d iD LP w3t6 Md air
dned b.fore listral inle.prclaion or lhe rcsults. ln posilivc smpld th. sile of anlieen_
anibody @mplex turn d sl.te bl.ck.
n Conoffilon ol AGPr .nd DL{. Sc6ilivily thtcshold lot AOPT 3.d DlA, lo deter Ag
rdd Ab of avian adc.ovirus isolate. was studied. Be$ out of thcEe lwo teds, wl used rar
d.t€lion or vi6l rntiscns lw.b i. @nmdcillly .vlilahl. HPSV vteim!
cl Con.lllEtion of Vird PrniclB in V|.cind: Four HPSV vaeines cohdcrcially
av0ihblc in rh. mirk.l (onpany A to onp..y-D) ed linh vaccinc, p.epared lrom HPSV
posirirc lez vs honoeen*c (posniv. conlrol), was iicluded in rhh stody Thc ldels of
viril anriacn!, in .ll olrh.$ vroci..s, w.c d.torniDed ro omptrc th. vdiltion in qu.lirv of
vaccin. from difer. muuflcluring udB.