HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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'l rbli Vlll. l. Scnrilivily ThBrhold ofAg.rGd PMipit ior Isr, ^g: Anris.n t^g)Co @tr ion r5% &oD.sri.i AAv i$ltG ojs.d in sP! chicts SPF chick B sdm n gdivc for HPS .nlibodid A.rig.n = 20,/" tiver hooog.nil. haling HPS vitus 5% 209l. l-ivd hotug@t. n.8td* lor HPS viru.. Andbody = Sdn hlviig li|lhodv .s.ini HPs Yi$ M*imuD dclelion ldel.
lrr T.hL vllr.I S.ninivny thBhoftl of Dot lmEu.obindtogA!!ry. l:20 l:40 l:40 l: l60 l:640 ts.rum, Ands.n (As) Con.qtr ioi 2O/o tao,4 t0% SAo 2,5% Contot ': I 80 dihlion of*tud,ri*d in SpF.hic!o, asainsr purificd pARC_l l$ld. D: I :30 diturion of&tun of SpF chickar neg.tiv. tirr Hps eribodi.s. Ag: AnriEd = 2O.d tjw. homogorrc having HpS vm3 ': 2Ctl. UB hohogicmlc n.garaw for HpS riru. ab: Antibody = S.oh iiised osainlr I+S vitus sions pdhiv. @lour on .irocdtd@ nM6r&c nnp ++ Moddrrc Dositiv..otour. + W.rk poliriv. color ": AnriCo @nc. of 2.5% .nd antibody ditldon of I I t 60 v$ obFR.d.
Page 1 and 2:
OBSERVATIONS ON CAUSATIVD AGENT(S)
Page 3 and 4:
"M sooD&{!B N|,lr97
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,/2 ./. -t4 In tlrc tnme of God. ll
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ACKNOIYLEDGEMENTS "Pro(hrm in th. n
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AC(NOWLEDGEMENTE LIST O[ TABLES LIS
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Cell cuhur. inocul io. PriMry viNr
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5. RJSULTS AND DISCUSSION coNclUsto
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EXPERfMENT TNL VIll S€tuo aitibod
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T N TRoDUc7IoN
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nds in 0ocks is th€ onty signilio
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R E v T E w o F L t T E R A T U R E
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highly d.trs I'otlrry ar@s in the i
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A l.orFdc Poi$n.: h) Chlorin.red hy
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Oc@ny (Vop, | 989) Tl'.ic qinr 6nro
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pig or rit. IndnEr ha.m.gsluri.0rio
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Mrner g, z/ (1989) iiocutded lh. su
Page 34 and 35:
virilotigin, perhaps an adenovirurw
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(AGPTT H.sn dr dl, t994) whit. d.re
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Trbl. l.t S.npd.l.!e or Ad.ldids Nu
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Th.s vinoN ae rcid sllblc (Hamparia
Page 42 and 43:
Tte viruss sharc a 6mnon goup antis
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Trbl. Lar S.relogi..l .l.ici6..tio.
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dnibodi., are rising in lield cas6o
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nun'ler ot polypenrid. bands derib.
Page 50 and 51:
Phosphokinls. ocrivity lssi.lcd wil
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sigm. Th.dis4e, whichcanbeqpe mcn$l
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nrrenar oibody vitu! wc i$hr.d fron
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Th. oain problcm with any sologidl
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obwed ihar AAv-polypaptidg could b.
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M A T E R I A L s A N D M E T H o D
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lrydld.tot tc.: Th. $dlr vq!.odn d
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Infation ofchi.k Th. experim.nht ch
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lhe monolaytr (3-5 diF), ihc 06ks w
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..diftg.d d 90,00 X Sb. 2. hor A ti
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Trta |lLr. lr..b.d.t D.Ln h. r.*. .
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EXPLR]MF.NT.IV BIOI,OCICAL CHAMCTOR
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^\]\ M.di.r tgg Inleriv. Doe (EIDq)
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nin Th.r. cells ud. slored.t -20'C
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EXPI;RIMLNT V CHARACTI]RIZATION OF
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El.ctrophoreb vs 6icd olt chhc. i.
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@ ainjr8 l./r gchriq dd inob.t.d fo
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ofPSS .nd srored at -70oC. Pr.&nce
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T.bb V[.], Etpdindtd d6ltr ofv.ccbr
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I;XPERIMENI.VlII A DOT IMMUNOBINDIN
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R EIUL7I A No o Isc u saIo N
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lrig.l, ll.Nrtor!chiclcn dl.d (72 l
Page 94 and 95: Fi8.3, Int.m.l or8.r of (hi.kdr,dl.
Page 96 and 97: ig.5. 8!6rotl.rbri.nBlronHPSig.ntin
Page 98 and 99: Fig. ?. Photodlcrcghpt! of h.rrr rl
Page 100 and 101: f.bL l-t. In nw ,a.l In utuo Ch.r..
Page 102 and 103: 24-48hK Absene ofhdctialgo*lh indic
Page 104 and 105: Rdlts ofrhis srudy ee ongodt wirh r
Page 106 and 107: lndlr.d nuorcsc.trl rntibody (IFA)
Page 108 and 109: PURIFICATION OFTIIE HPS VIRUS men.e
Page 110 and 111: da'dy of l.3s tnto|f i' c|O. noF€
Page 112 and 113: T.bL tl!l. Mo.t lit h DFiL. cti.t .
Page 114 and 115: Pithogmic in rni! nudy lhould ody b
Page 116 and 117: T.bl. w-r. Ell6t ol C.tl Cutrrn psh
Page 118 and 119: The anrier., ftis.d agrinsl purifi.
Page 120 and 121: T.bb rv.3. fttldl .rd Cr.r&.t Cr.st
Page 122 and 123: T.hL lV-4. Virur n.uhlttiio. suhi S
Page 124 and 125: EXPE]IIMLNI.V CII RACTERIZATTON OF
Page 126 and 127: SDSPACE shorira con0uison oa l l *r
Page 128 and 129: Fis -Va rlso indiqt. that in &mtyp
Page 130 and 131: a ibodt in 6 erun of low iite. The
Page 132 and 133: Thc resulls ofSDS-PAOE ds re{@Gd ll
Page 134 and 135: Trbl. Vll-|. S.tun a!.ibodid rg.inr
Page 136 and 137: s 225 175 125 tm 25 :i--.'' i,; \\
Page 138 and 139: T.hl. vl|,3. 0oir.hrllcng. Mort.lly
Page 140 and 141: Tlblc Ml,4 indic.t6 lhd lhc pdort?c
Page 142 and 143: I chdt.ng. t.ot.r.. Fls.Vll,3. trcr
Page 146 and 147: Tabh. VIII. 2. S.nlitivhy threlltol
Page 148 and 149: T.blq Vlll. a. D.lalion oa vinl .nt
Page 150 and 151: n g.tive re;ons Althoush a dositomd
Page 152 and 153: SUMMARY llydropericarditrm lrndr6oe
Page 154 and 155: c o N c L U s I o N s
Page 156 and 157: R E c o n M E N D A 7 I o t I
Page 158 and 159: L T 7 E R A 7URE c I7ED
Page 160 and 161: Arhr,s,s z idulMI xt'r ' lrez. Risr
Page 162 and 163: Cool.lK.A l9t2 Avi,nl|.miGiLturbnor
Page 164 and 165: Gim,TM,DJ Kil!andsH xkva le?6 ^prli
Page 166 and 167: Mirtrtrood, F, RU. Sh,r,id, Mz Ktun
Page 168 and 169: ftrcifl, H 6 1156 n?ii! of ldctuida
Page 170: v.D Ecr, J.H H . ll.C DiErsr, rA M
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HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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