HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

ISOLATION AND PNOTACATTON OF EPS AGENT
Sou.c. of vitut ttrd it! prop.grtlonr Fivc isolats of AAV wde r@v.rd from liv.r
homog.. e or ihe bnds died of HPS lolrowing srr.drd p.e.dur* (Nadm d/ at., | 995).
l$la|e A & B vcrc broild i$lrt.s, C & D w. bt4ds i.ol 6 ald E wls prororrp. ishc
A 20./q hoooscml. fron liE v.r prcpar.d in phosphlre buF.r $lin (pH 7 2) wirh
lddirion of p.nicillin sodiun (1q000 | U/Dl)r ltEplomyci. slphal. (2 hs/ml)r s.nllmicin
suhhde (1.0 mglml), t.namycin suhh.t. (065 ns/ml)i and mpholdicin B (002 ng/nrli
S.nne, 1939). The homo8oab w!! c.ntitugcd it 3,000 rp6 aor 20 ninutes in ! rofiigdatd
c.idtuge (4 lo'C) to editunl li$u. d.bis rnd my b.ct.ria. Thc euhi.s $pendbnt *as
.*prqlly renov.d .nd 6lr6ed rhrouAh 0.,15 ..d 0 2 mic.on 6lG. The fiXrat. s3 funho
@rifuged a125,000 rpo (30,000 x B) lor m miorB Th. p.lld wN esp.ndcd i. PBS (pH
?.2) aDd fhcred rh'ough lm nm filla. ro.tlo* only $. p.ss€ oatitus Th. 6lt rc wr!
lrd for ielllioo ofvirus lhrou8h lhc i,nlowing proc.du6:
lll C.ll cultur. inodlarion
A total or l0 clinically nomd broild cliiclo (Hubbd x Hubbed) $. .9. of 25
drys ws. obtdned frcn . lo@l hlrch.ry. Th. chictr w.rc id€dined by winS bdding ind
divid.d i.ro rhr@ ercup3 ach oBining of id broil6s. Aft.r rt:trfd 10 1fi. isol,tion nnir .f
rhe Anio.l Healtti Lib., Aninil Scid€ l6litur., Nliionrl Agdoltunl R@rch Cotrq
lslanab.d, individu.l sa oflh. ohick v@ indyz.d by aed s.l
l9?3) !o dctcmiie preiioelarion antibodi.s
pr4iDitrtion Lst (Ctowl.,