HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

nun'ler ot polypenrid. bands derib.d for ormmdiln adenovitu*s viris in dillsmr
sludi*. Th€ b.nds sr. dor. rhi. len ud d. dep.deol on rh. ssorype i.d r€hniqua
mproyed How.vcr, th. lvie &tenovirus CELO stBin (FAv_ r ) w!! show b hlve . salle.
number ofpo!?rptid.! in a shdy byk!.reral (1971) white Li 314l (l9s4a) obsetoed ar
leesr 14 srtoctural prol.ins. Th. MW ofslncrural polyp.plid.s variq ftom 5 KD lo l2O KD
(Nonnys,d/, I976:wisonds/al, r932)andtorFAv6KDro toor\DGieral, l9s4!)
Ad.novirus iyp. 2 virioB in sodiun dod@yt $tDh.G Doty0crytlmid. gcl
eleckopho.csis (SDs PAGE) rcvqlcd 9 to 12 potpeplid6 witn Mwr:s8ing oom I xD to
120 KD (Evdiu er zl, l9?r; tudslon e/ 4/., l9?1, N.rftul, l gsoj Nqmul, l9s7)
Vnd polypeDlids (\?) , Ill rV,Ilta, Vt, V0t end tx consritur. orarc Mciired
witr! the thre stodurat sbunirs oa.ddovirus viz. h*o,, p.nlon brse and ribE (Norby ei
dt , t916, \|igand et al , 1932).
Th. hqon n i t.intr of rtn4 ide.riat potrTepride mdeuls (\? rD Vn l
polypepnd. n has a MW of t08 KD. ,Thc primary structure ofrhis potypeptide suggesrs thar
thl confonr ion oarhc middt. ponion is ditt rcnr tron thii 6frhe rwo .nds, potyp.pride
VlIl (lr KD) lnd po$ihly \,P \rI . 24 KD prot.in is .ts hdon eodcd. potypcp.id. ItIr
(66 kD) L N$chted qirh rhc pqip.ntonll hdoE (Eqill ./ aa, l97j; N.tur, r gso)
Ths penton capsom€rc is a conptq ofDenro. bar..nd fibre polyp.ptidd Tbe !.nton
bas. cotuhts of tive subunil3 of VPt0 (85 XD) which h altach.d lo rtE fibrc (rim.r of
poly!.pride lv, 62 KD) Th.libr. consisrs ofa shln wi$ a r.mio.t tnob (N.mut, lgso and
r93?)
Ar lan l4 strudurltproriB wirh r @g. of MWs lron too KD to 6 KD h!v. bq
id.nrified in CELO vinE (Lierat, l9s4a, N.mut, 1987) Tbe CELO vitus core cont.ined
polypeptid.s IX, xar and xlt rhal .r quire ditrer.nr from tnoE rn hund adenovituses
(poryFpridc v rnd Vlt) (Li ., al, l9s{h). Th! ehcrural potyp.ptid* of CELO viG dd
ricir M.wi &e VP II tOO KD hdo., \/P II 92 (D p.nlon, Vp IV 65 KD lona libE ,nd Vp
lrII 44 5 KD shon ffbr. vimt potypcptid. v, Vl, vII!, x, xflt and xtv havc MWs of 62,
55,30, 13,9 and 6 KD rcspectvety (l.ic, dl, 1984a)