HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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..diftg.d d 90,00 X Sb. 2. hor A tiu h.dt.! it in n. dhidb -d L*E dcuitio *r, c.tet'r 1&. b|.d v|r pn-d !n c"5o o&* $dd.x udls rln be.. fi. prli6d .4do v.r di|d ft. rittE Dt d.!ru oioeo0, .Dd .t ivrdor g4 tEd.d. Yrr l.!.fui.. td E !r.d ro ddy ir ttF It iD iio..roo!io! w i..ol..d ro lO etEedtL k!it- dbb !o r.. it F!$dtD !.!.n md E @ft@d ly A(P t a (qfi,b, l9D).
EXPEAIM<strong>IN</strong>T.III ROUTf,S OT |rPS <strong>IN</strong>FEqIIOit v'ascs nq mre i.to rhe body ofth. h$t by various .out.s sch !s via relpnrtidn, inScslion, bns or oechanical Denetadon. The frequency of virus inplanralion is grearesr wh.G tifls @m6 in di@1 6 lcr uith |h. livins clh $cfi $ .6Pr!to.y tid. Ta d.r.rmin etrect ofinlslion routc olHrs in bnds,lh. follo*ing expe.iMt wa d6ign d. Ti$€ dlrur. propasat d vitus $sp€cion of hydropeio.dion syndro@ vioi GfSv) wa! seoipuiified by the procedurc derih.d by Naeo 3/ dr (1995) wirl $me nodifiqrioB Bd.fly, rh. cldn€d ris$€ cuhure pottg{.d titus homogd.t. (l0o nl). w.! lih.red rhrough 0 3 !.d 0.45 difioocter 6hcrs &d wB $.d in rhir dpcdmenl A rorat oa 120 conmtionltly r!L.d 2 @ls-old broild {Hubhtrd x Hubbard) chickds plocured fiom NtS SB Chicks (Pvt.) Ltd, Rawalpindi, Pakistin, Th.sc bndr w.re lhd tMdonly alloc ed rc !d.n SMps oa20 bnds 4cn i. .n i$lalion unil Aninal llous, Anim uealth Inrrirur., NARC. klsmcbld Bnds w... val.red dd f.d ad /,rir,' An inocuhrm of O.5 6l ol vitus $spc6ion onl.inins 10" nedian i$uc cuhu. infedive doses ('lCIDs) ws administer.d lo the bnd, sroup A-D, rs shovtr in Tablc UI I Thca. groups qs. hous.d roBclhd ud wilh E Soup u'i.odlol.d '@nl!cl birds TIe F s.oup (6ntoD wrs .dddltcr.d o 5 nl ofMEM md t.pr in sdcr isola.ion hul sith sinil& Bi.d! ws. ddincd cliniqlly 4h day dd lny dad ttt& v@ ebj4led |o posl nodem ex.ninarion, including rhe hktolosical mminalion ofth. el&ted r6ua\ op lo l0 days po* inoculalion (Pl) BIood was @lleclcd aron birds co$idercd motibund ed thcv
Page 1 and 2:
OBSERVATIONS ON CAUSATIVD AGENT(S)
Page 3 and 4:
"M sooD&{!B N|,lr97
Page 5:
,/2 ./. -t4 In tlrc tnme of God. ll
Page 8 and 9:
ACKNOIYLEDGEMENTS "Pro(hrm in th. n
Page 10 and 11:
AC(NOWLEDGEMENTE LIST O[ TABLES LIS
Page 12 and 13:
Cell cuhur. inocul io. PriMry viNr
Page 14 and 15:
5. RJSULTS AND DISCUSSION coNclUsto
Page 16 and 17:
EXPERfMENT TNL VIll S€tuo aitibod
Page 18 and 19: T N TRoDUc7IoN
Page 20 and 21: nds in 0ocks is th€ onty signilio
Page 22 and 23: R E v T E w o F L t T E R A T U R E
Page 24 and 25: highly d.trs I'otlrry ar@s in the i
Page 26 and 27: A l.orFdc Poi$n.: h) Chlorin.red hy
Page 28 and 29: Oc@ny (Vop, | 989) Tl'.ic qinr 6nro
Page 30 and 31: pig or rit. IndnEr ha.m.gsluri.0rio
Page 32 and 33: Mrner g, z/ (1989) iiocutded lh. su
Page 34 and 35: virilotigin, perhaps an adenovirurw
Page 36 and 37: (AGPTT H.sn dr dl, t994) whit. d.re
Page 38 and 39: Trbl. l.t S.npd.l.!e or Ad.ldids Nu
Page 40 and 41: Th.s vinoN ae rcid sllblc (Hamparia
Page 42 and 43: Tte viruss sharc a 6mnon goup antis
Page 44 and 45: Trbl. Lar S.relogi..l .l.ici6..tio.
Page 46 and 47: dnibodi., are rising in lield cas6o
Page 48 and 49: nun'ler ot polypenrid. bands derib.
Page 50 and 51: Phosphokinls. ocrivity lssi.lcd wil
Page 52 and 53: sigm. Th.dis4e, whichcanbeqpe mcn$l
Page 54 and 55: nrrenar oibody vitu! wc i$hr.d fron
Page 56 and 57: Th. oain problcm with any sologidl
Page 58 and 59: obwed ihar AAv-polypaptidg could b.
Page 60 and 61: M A T E R I A L s A N D M E T H o D
Page 62 and 63: lrydld.tot tc.: Th. $dlr vq!.odn d
Page 64 and 65: Infation ofchi.k Th. experim.nht ch
Page 66 and 67: lhe monolaytr (3-5 diF), ihc 06ks w
Page 70 and 71: Trta |lLr. lr..b.d.t D.Ln h. r.*. .
Page 72 and 73: EXPLR]MF.NT.IV BIOI,OCICAL CHAMCTOR
Page 74 and 75: ^\]\ M.di.r tgg Inleriv. Doe (EIDq)
Page 76 and 77: nin Th.r. cells ud. slored.t -20'C
Page 78 and 79: EXPI;RIMLNT V CHARACTI]RIZATION OF
Page 80 and 81: El.ctrophoreb vs 6icd olt chhc. i.
Page 82 and 83: @ ainjr8 l./r gchriq dd inob.t.d fo
Page 84 and 85: ofPSS .nd srored at -70oC. Pr.&nce
Page 86 and 87: T.bb V[.], Etpdindtd d6ltr ofv.ccbr
Page 88 and 89: I;XPERIMENI.VlII A DOT IMMUNOBINDIN
Page 90 and 91: R EIUL7I A No o Isc u saIo N
Page 92 and 93: lrig.l, ll.Nrtor!chiclcn dl.d (72 l
Page 94 and 95: Fi8.3, Int.m.l or8.r of (hi.kdr,dl.
Page 96 and 97: ig.5. 8!6rotl.rbri.nBlronHPSig.ntin
Page 98 and 99: Fig. ?. Photodlcrcghpt! of h.rrr rl
Page 100 and 101: f.bL l-t. In nw ,a.l In utuo Ch.r..
Page 102 and 103: 24-48hK Absene ofhdctialgo*lh indic
Page 104 and 105: Rdlts ofrhis srudy ee ongodt wirh r
Page 106 and 107: lndlr.d nuorcsc.trl rntibody (IFA)
Page 108 and 109: PURIFICATION OFTIIE HPS VIRUS men.e
Page 110 and 111: da'dy of l.3s tnto|f i' c|O. noF€
Page 112 and 113: T.bL tl!l. Mo.t lit h DFiL. cti.t .
Page 114 and 115: Pithogmic in rni! nudy lhould ody b
Page 116 and 117: T.bl. w-r. Ell6t ol C.tl Cutrrn psh
Page 118 and 119:
The anrier., ftis.d agrinsl purifi.
Page 120 and 121:
T.bb rv.3. fttldl .rd Cr.r&.t Cr.st
Page 122 and 123:
T.hL lV-4. Virur n.uhlttiio. suhi S
Page 124 and 125:
EXPE]IIMLNI.V CII RACTERIZATTON OF
Page 126 and 127:
SDSPACE shorira con0uison oa l l *r
Page 128 and 129:
Fis -Va rlso indiqt. that in &mtyp
Page 130 and 131:
a ibodt in 6 erun of low iite. The
Page 132 and 133:
Thc resulls ofSDS-PAOE ds re{@Gd ll
Page 134 and 135:
Trbl. Vll-|. S.tun a!.ibodid rg.inr
Page 136 and 137:
s 225 175 125 tm 25 :i--.'' i,; \\
Page 138 and 139:
T.hl. vl|,3. 0oir.hrllcng. Mort.lly
Page 140 and 141:
Tlblc Ml,4 indic.t6 lhd lhc pdort?c
Page 142 and 143:
I chdt.ng. t.ot.r.. Fls.Vll,3. trcr
Page 144 and 145:
'l rbli Vlll. l. Scnrilivily ThBrho
Page 146 and 147:
Tabh. VIII. 2. S.nlitivhy threlltol
Page 148 and 149:
T.blq Vlll. a. D.lalion oa vinl .nt
Page 150 and 151:
n g.tive re;ons Althoush a dositomd
Page 152 and 153:
SUMMARY llydropericarditrm lrndr6oe
Page 154 and 155:
c o N c L U s I o N s
Page 156 and 157:
R E c o n M E N D A 7 I o t I
Page 158 and 159:
L T 7 E R A 7URE c I7ED
Page 160 and 161:
Arhr,s,s z idulMI xt'r ' lrez. Risr
Page 162 and 163:
Cool.lK.A l9t2 Avi,nl|.miGiLturbnor
Page 164 and 165:
Gim,TM,DJ Kil!andsH xkva le?6 ^prli
Page 166 and 167:
Mirtrtrood, F, RU. Sh,r,id, Mz Ktun
Page 168 and 169:
ftrcifl, H 6 1156 n?ii! of ldctuida
Page 170:
v.D Ecr, J.H H . ll.C DiErsr, rA M
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HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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