HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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Trbl. Lar S.relogi..l .l.ici6..tio. of .v1.. .doorirqa. Itgtg Ittta @ SB }l'ad r|mI.r AfrrL! tr. Slr i Adspted from McF.dtn (l99l ) US,r {o..ry R.!rrdi.. ''1- :" : "l : c
.teoper0lurein cxcessof22 oC ise$enrid Wittiams0nd Sieset(1969) hive recomnended a lemp€ralurc or2l lo 24 oC md 70 per ent hunidity for etrarilc tormatd.hyde tumisaiion Trkey Hmmorhnsic Enr6ilis (THE) vid3 was thoM b be lcnliriv. ro , ons. oa disinferlnrs.hhlush.ll we6ls efdiv. i. pr.se or s.run (Oommurh dd Cros, t9?l) Anong rh. convenional fowl idcnovirus.s (FA9, hredlSSluinirion (HA) has bei demoNh@d only wirh FAV r, vhich.galuriurd orly n..ryfi.ocrre (McFeau lnd Adair, 1977) Eeg dro! syndr6mc (EOS) virus lsslulinatB chick.n, rurkcy md duct eryrhrc.yrg (Adan dr ol, 1979) nalins Hl i ls.tuIidi lor s.ologicll idctuification oaEDs Seveml anrisens h6ve ben lecosnizcd and char.cteized m adenovi^u panicles (McFenan, l93la) The lyp. sp@ific a.tisen sv.s ris. to a ibodi6, which neralize virus infectirity and n th.rforc ls.tuI for cl.$ifi€tion ol i$ht6 into $oryp.! (Tible | 4) Thc srouD- sD*i6c antisc. givc! .i$ ro erjbodi6, which eci in ID, cF or IFT wir[ orhd s.orypcs wnhin lhe gcnu5: i k r.sponsible ror dD nijor division oflh. aanily inro Mastadenovinses a nd Aviadeioviruses Thb *s blsed o n rhe ob$ Raio n rhat th e grou p 3p ecillc antisen shared by the coNenlional FAVS did not cross r.act i! ID t6ts with that of the namroli.n adoo;tu!.! (K.wrmun e, al, l 4; McF.nrn e/ 41, 1975). Commo. ..rigds h.E ben dcmonnntcd i' FAv dd EDS viDs itrlda.d ell! adendale, I9?3) allhough rhey @n.ol bc d.mo.{aled dnally lsitr8 ID as wirh sN.niioml FAvs (Adan er 44, 1979) Comnon dtigeos have bccn dcmdnnrakd by indned means (McFerm et at, 19731)r onry bnds which have .rp.i..c.d nulliple FAV i.'f€tions, lnd which posss hish ls.h of aibody b ine IAv Stoup{l*i6c dtigd will ccs racl wnl EDS vitus by IFT or ELISA (Adii. ?r 41, 1936) Th. 6mmon ddemiMntt are prob.bly presnr in only sm.ll amounc which is tunher sbsr.nri.td by rh. obsnalion rhd sroup sp.oillc p.ecipitin racriom ro 0d.novirus incEase ar d'c a{mc ti'ne thar EDs vitus
Page 1 and 2: OBSERVATIONS ON CAUSATIVD AGENT(S)
Page 3 and 4: "M sooD&{!B N|,lr97
Page 5: ,/2 ./. -t4 In tlrc tnme of God. ll
Page 8 and 9: ACKNOIYLEDGEMENTS "Pro(hrm in th. n
Page 10 and 11: AC(NOWLEDGEMENTE LIST O[ TABLES LIS
Page 12 and 13: Cell cuhur. inocul io. PriMry viNr
Page 14 and 15: 5. RJSULTS AND DISCUSSION coNclUsto
Page 16 and 17: EXPERfMENT TNL VIll S€tuo aitibod
Page 18 and 19: T N TRoDUc7IoN
Page 20 and 21: nds in 0ocks is th€ onty signilio
Page 22 and 23: R E v T E w o F L t T E R A T U R E
Page 24 and 25: highly d.trs I'otlrry ar@s in the i
Page 26 and 27: A l.orFdc Poi$n.: h) Chlorin.red hy
Page 28 and 29: Oc@ny (Vop, | 989) Tl'.ic qinr 6nro
Page 30 and 31: pig or rit. IndnEr ha.m.gsluri.0rio
Page 32 and 33: Mrner g, z/ (1989) iiocutded lh. su
Page 34 and 35: virilotigin, perhaps an adenovirurw
Page 36 and 37: (AGPTT H.sn dr dl, t994) whit. d.re
Page 38 and 39: Trbl. l.t S.npd.l.!e or Ad.ldids Nu
Page 40 and 41: Th.s vinoN ae rcid sllblc (Hamparia
Page 42 and 43: Tte viruss sharc a 6mnon goup antis
Page 46 and 47: dnibodi., are rising in lield cas6o
Page 48 and 49: nun'ler ot polypenrid. bands derib.
Page 50 and 51: Phosphokinls. ocrivity lssi.lcd wil
Page 52 and 53: sigm. Th.dis4e, whichcanbeqpe mcn$l
Page 54 and 55: nrrenar oibody vitu! wc i$hr.d fron
Page 56 and 57: Th. oain problcm with any sologidl
Page 58 and 59: obwed ihar AAv-polypaptidg could b.
Page 60 and 61: M A T E R I A L s A N D M E T H o D
Page 62 and 63: lrydld.tot tc.: Th. $dlr vq!.odn d
Page 64 and 65: Infation ofchi.k Th. experim.nht ch
Page 66 and 67: lhe monolaytr (3-5 diF), ihc 06ks w
Page 68 and 69: ..diftg.d d 90,00 X Sb. 2. hor A ti
Page 70 and 71: Trta |lLr. lr..b.d.t D.Ln h. r.*. .
Page 72 and 73: EXPLR]MF.NT.IV BIOI,OCICAL CHAMCTOR
Page 74 and 75: ^\]\ M.di.r tgg Inleriv. Doe (EIDq)
Page 76 and 77: nin Th.r. cells ud. slored.t -20'C
Page 78 and 79: EXPI;RIMLNT V CHARACTI]RIZATION OF
Page 80 and 81: El.ctrophoreb vs 6icd olt chhc. i.
Page 82 and 83: @ ainjr8 l./r gchriq dd inob.t.d fo
Page 84 and 85: ofPSS .nd srored at -70oC. Pr.&nce
Page 86 and 87: T.bb V[.], Etpdindtd d6ltr ofv.ccbr
Page 88 and 89: I;XPERIMENI.VlII A DOT IMMUNOBINDIN
Page 90 and 91: R EIUL7I A No o Isc u saIo N
Page 92 and 93: lrig.l, ll.Nrtor!chiclcn dl.d (72 l
Page 94 and 95:
Fi8.3, Int.m.l or8.r of (hi.kdr,dl.
Page 96 and 97:
ig.5. 8!6rotl.rbri.nBlronHPSig.ntin
Page 98 and 99:
Fig. ?. Photodlcrcghpt! of h.rrr rl
Page 100 and 101:
f.bL l-t. In nw ,a.l In utuo Ch.r..
Page 102 and 103:
24-48hK Absene ofhdctialgo*lh indic
Page 104 and 105:
Rdlts ofrhis srudy ee ongodt wirh r
Page 106 and 107:
lndlr.d nuorcsc.trl rntibody (IFA)
Page 108 and 109:
PURIFICATION OFTIIE HPS VIRUS men.e
Page 110 and 111:
da'dy of l.3s tnto|f i' c|O. noF€
Page 112 and 113:
T.bL tl!l. Mo.t lit h DFiL. cti.t .
Page 114 and 115:
Pithogmic in rni! nudy lhould ody b
Page 116 and 117:
T.bl. w-r. Ell6t ol C.tl Cutrrn psh
Page 118 and 119:
The anrier., ftis.d agrinsl purifi.
Page 120 and 121:
T.bb rv.3. fttldl .rd Cr.r&.t Cr.st
Page 122 and 123:
T.hL lV-4. Virur n.uhlttiio. suhi S
Page 124 and 125:
EXPE]IIMLNI.V CII RACTERIZATTON OF
Page 126 and 127:
SDSPACE shorira con0uison oa l l *r
Page 128 and 129:
Fis -Va rlso indiqt. that in &mtyp
Page 130 and 131:
a ibodt in 6 erun of low iite. The
Page 132 and 133:
Thc resulls ofSDS-PAOE ds re{@Gd ll
Page 134 and 135:
Trbl. Vll-|. S.tun a!.ibodid rg.inr
Page 136 and 137:
s 225 175 125 tm 25 :i--.'' i,; \\
Page 138 and 139:
T.hl. vl|,3. 0oir.hrllcng. Mort.lly
Page 140 and 141:
Tlblc Ml,4 indic.t6 lhd lhc pdort?c
Page 142 and 143:
I chdt.ng. t.ot.r.. Fls.Vll,3. trcr
Page 144 and 145:
'l rbli Vlll. l. Scnrilivily ThBrho
Page 146 and 147:
Tabh. VIII. 2. S.nlitivhy threlltol
Page 148 and 149:
T.blq Vlll. a. D.lalion oa vinl .nt
Page 150 and 151:
n g.tive re;ons Althoush a dositomd
Page 152 and 153:
SUMMARY llydropericarditrm lrndr6oe
Page 154 and 155:
c o N c L U s I o N s
Page 156 and 157:
R E c o n M E N D A 7 I o t I
Page 158 and 159:
L T 7 E R A 7URE c I7ED
Page 160 and 161:
Arhr,s,s z idulMI xt'r ' lrez. Risr
Page 162 and 163:
Cool.lK.A l9t2 Avi,nl|.miGiLturbnor
Page 164 and 165:
Gim,TM,DJ Kil!andsH xkva le?6 ^prli
Page 166 and 167:
Mirtrtrood, F, RU. Sh,r,id, Mz Ktun
Page 168 and 169:
ftrcifl, H 6 1156 n?ii! of ldctuida
Page 170:
v.D Ecr, J.H H . ll.C DiErsr, rA M
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HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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