HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

n g.tive re;ons Althoush a dositomd.r hrs b.en ur.d to r@rd the rc$hs (Futoya c/ o/ .
1984), uilny oaDIA !r! routin. ff.ld t.n would n*d a simpl. inl.rpretalion
Nordous r.pons o. hydro|qiordium syndomc (lrPSI h.vo @@lcd a npid
inc.d& in ir s i.cid.nc in ind oursid. P.kiitd Duc ro l.cl ol c.ll alror. Lcilit ia in mod or
E d i3ea3e di.glost ic laboiitories, thepa.rceofHlS isconnmd by as& gcl precipitation
t.st (ACPT) or by.4ro'3y findings On th. othe hdd nonc ofthe approp.iatc mdhodolog
to eddr. ihe ommdcid HPSV vd€i6 hlr ben rd+t.d by, rh.8ov@t of Plkien
Errli.r, r.nztnllinkcd imnnosorb.nt i$ay (ELISA) e$ d.snb.{t ror th. dcrerion of
rniibodier tgri.* HPS (Ih$u d al, 1994). Allhough rh. ELISA provid.r v.lurbl.
di.gnorric Dromrtion on lvia ldcnovirus, ihe lime dd th. cosr lnd nrlur. of equipdcnr
involv.d in ottaining rdlB .nd lhc spcifioty md olititit, ofELISA are lh. inpodr
limning fdctoB aor thi! r.chnoloSy.
E zymelink.d innunobinding !$iye senerally uso polystyoe lnd sinilar ma&ials
lh.t h!v. a low bindi.g oprcity for prot.i$ Tne dctction ol proi.jm by $c w61cm bloi
kclDique following poly.crylmide scl .l.ctrophordis har dmonsrlred rh. ruDqior bindi.s
caFcily oaproteins to nitrocellulos m.nbmre. Tnh propcny h.s bd qploh.d by ,eved
r.e.crch*s ro dot ctude .viml sndgc$ di@rly oito nilroc.llulo$ prp.t lot h sjtt
visuslialion of .ntis.n (As)dtibody (Ab) 6frplq6 witi cn4nelinkd imm!@gtobutin3
rnd ! tubstarc, vhich loms .n in$lubl., @lord dd-prodlcr (Towbin er 41, | 9?9; Hawkes
et al , \9a2.,Fntuy^ el 41.,\9a4: Gordon and Bitins. 1933i Heblrlilg er 4t, 1938; Muner e,
al, 1939 and Naeen and Coral, l99o)