HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...
HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...
HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...
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lhis oonolayer boundlriq of indilidud c.ll! w... indistirct and ih. 6.ten|' appded lainit,<br />
The HPs vitur i$lrrcd lion infeid chidds lnd ECE r.pliqed rcll in bofi CEL<br />
dd chic*cn embryo kidncy (CEK) cllaltuB Th. hiddt vitus titr6 we ohlaio.d usi.e<br />
orads ftom l6-day{ld cmhryos. Howcv.r, th. HPSV did nol ause any siBnincr.r CPE in<br />
chicken .dbry6 6broblin (CEF), orso. cunure, qudl l.nicl. iQT) c€lk 6. vtro cell lincs<br />
For dis.gaEgarion of.piih.lill elh of livcr ind kidn.y, seve.al perqrogd i0 0t<br />
l%) oflrypsin (Rith or wnhoul ved.n.), p.ovidins oold and hot envi.onmenh wa8 usel A<br />
0 5%co.cent.rionolqpsinar25'C lor I nr, dhcggr.e ed celhcompletely<br />
ln living prepararions obscRcd undq lh. micoscope lo*power maBnifictrtion, rhe<br />
f*t noticohle change i6 inletcd cultlc (CEL) wd een !t 18 hts Pl phen rhe bound0rie!<br />
of mny cells in the cpilh.lial ares ippe.r.d dilrinct ind ofr.. ihilke.ed 6mpi€d with rhc<br />
@dDls The.e was .ounding and nod..u. lwclli g ol i.i..t.d @lls which {nded at dJour<br />
24 hB li€r inoolarion Th. Mll.n ccll! botu tlfirclile and detached from rhe slas or<br />
polyiyr.n surfa@ uilhin 48 hB posl-infcdion. Thi cPE w$ fid rc$nd.d to<br />
ci.omsc.ibed ar4 larsely ro rhc .pilncti.l elb Thc fibrobhss h.lNd $e .pnhe[!l Gllndt<br />
we.. la'gely nninfdr.d Ldg. numbc* ofroundd c.lh in Srapc-like cluslqs we.c pr.s..r i"<br />
fic nedium la1€r il exl.ndcd lo th! whol. cul|urc, reslhing in 100% CPE ber!*n 72-100<br />
hs posl-inreclion Pliqug prodlc.d by rins in CEL ccll culture wqe both mall and lar8.<br />
Plaque beaa' ro appear 6 days afte. inooul ion and becam. aboul I .o 4 nn in dirmeter on<br />
Th.findings ofoui study ri. congru.nr with those rponed by Aghakhan (19?4) and<br />
McFeran (1980) who obsery€d thal lh.chick.n lddoviruss repliare hetter in honolqous<br />
than in heteroloeous ceu oltur. systetu Ad.noviN isolares oa chick.n oigin prop.g'te<br />
r..dily in €hic*.n kidney elt cnd ob.yonic lidnq Dd livd celh<br />
Tle 16 Gofphqu.fom ion trr. rlso sppo.l.d by lhe oh$N0rions ofcnomi.k d,<br />
at ( 1 96 1 ) *ho Eoon.d rhot rhq. is d.v.loptu of pl.qus i. CEK cells overl.id by igr<br />
and en h. @Dn.ed 5 days postin@htion of th. viD!
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