HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

A DOT IMMUNOBINDINCASSAY FOR IIPS ASSOCI TED AI'IAN
ADENOVIRUS
Of rh. larious Mrigen con6r.alioN ol l llSV liver lrnog.n0t$ I 5% w.s ddanable
in AGP t.sl wirh | 20 sedm dilutioo (T.bl. Vll!l). O.@ntnry,2 5%.nli8 con€ntalion
wls det.ctrbl. wnh smm ditulion up lo I 160 (T.bl. \4U-2)
EnzF. inmuno.sys gcrcnlly ue s.om diluliont of | 50_ ! : 100 os sctrn'Dg a$rv!
ro elimiMre nonsp.ciic @.lbc obs^€d .t 10*6 dilution3 Endpoinl lihtion with vlious
anrig.n ud sc.a 6nc.nr6ioN indial.d ihd lhe tcnlitititv ot dor immunobindins .sv
(Dl A) was almos 6-fold srdq rb.n t|[l of the ACPT {Tablo vl u_3 )
L.vel ofvn.l !nri8.ns in four oodmcrciauy avtil0bl. HPSV laein.s was obseryed ro
hc qun. variable vith a shndnrd etun dilotion of I : ! 60 in DIA (T!bl. Vlll _4) Thi5 ltn al on
i.! ia.n 6n@nt.lioB olommftill HPsv viaim! on onc h|nd indist.d ditra'n6 i'
pcr@nraBc oaanlrscnic rorcrioh ulaj *til. rokin8 v.cinc! dd on lh. othd hmd dcpicl'd
lack ot quahy conlrol nan.q .t mrnuficiuring untrt
l. pr.sent *o.l usi.slhc AAv i$hte dols on nn.ocellulde (NC) sttips a simple dot
innuiobinding sMy {DlA)
for lhe raPid d€l.dion of vnd a'li8'n and antibodic! wilhoul
rh. n@d for conlainnenr or o$er {@ial equipmenl, Ms d€!.loped The purpose of thh
rcrt wc ro inGtigitc rh. v!h. or lh. Dl^ modifi.d form oa ELISA, lor rhe dcterion or
'
Nirocellulos. o.nbM.s h!rc 0 sttons p'ot.in bindi'8 dprcitv ind hnv' ben used
tor immunoblotring ol Proteins CouPl.d wilh ELISA' nnrocelluloF I'as bcen und
slcce$tully (o develop DIA for rhe delection of vitious vntl anlibodt/anrisen svsrcms (Bode
4 al , l9s4) The cxkftly Io{ 4nenk6lions of neukalizing anibodv in PARC_ l iDrecrions
.nd fi. laalhy tnd sophi{ic.t.d .quiPMldcp.ndat pr'tmls of nindlrd ELISA 'nd
ACPT cnourlsei lo dddop DIA for lh. ripid d.tclion ol PARC_ l AYAb The t6t ai b'
@mpletcd in I 5 hous dd alloss l4lins of I or moc n@inadsed al one lmc