HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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Tte viruss sharc a 6mnon goup antiscn distncr liom Crotrp ! rkd.iolitu$ (T!bh rt EDS 1976 (FI27) is the trinciDal pathogen in 8r@p lU ind is serolosically id.ntial wi$ the ielatcs lion wlefowl, which @ coNidscd to h. rhc pro3ditore ol EDS 76 Crcup I adenovituss @i be subdivid.d into 12 *otlT* (Tibl.l.4), which.re cxrensisly disriblrcd arcns @oocrcial chicks FAVI, of\4'ich CELo i! th. ryP.3t in, is a.tige.i.ally rehted lo QB vitus. The Tipron strin, respoBiblc lor IBH in rh. U.n.d Sta.es, is cl.$ified $ a Europed erotypc 5 (US seto.ypc 3) Physicd r.d Ch.oi..l Ch.mctdirti6 oaAvirddoviruB Thc stuotu.e or cdenovio rcr is *.ll d efined by elecron mico tcope (EM ) Th. virus prni€lcs .rc icosihcdral .nd nedrc ?4-30 nm in diands Th. 6pson6€! .rc atugcd 'n tiangular facets qi$ sx crpsmet s along .ich edS. with most pr.par.tioB of EDS tios individurl spsoncrd 6i, bc difhal o 'caolv€. How.vci, Kan sr al (1979) wq. .bl. rd dd6nst6le panicles wnh rlTial addovi mQholosJ The fowl .doovirus hav. iwo fibres poj*rins from .00h vett* *hered EDS lirus has onlv one nbre (Gcld'6lom and M.ichteldppe. l9s2) virus Plrlicl6 and a$ocialcd irclunoB Q. b. dcmoshied i. the nrcleus by thin seclion and nutlar inclusioN by haemalaxvlin and @sin (u&E) slai.in8 aid imouoonuor4enl le{ (IFr) Ad.noviruss snibn r hig! d.gd ol Gillrn@ to ! sid. mg. of phvsidl dd chenical igenls: most ar. stable on horinglo 56'C; thhouah someisolaks ri. inaciivaled a1 50 "C (McFem. and Adtn, 197?). Ess dop svndrone (EDS) virus inf@ viry is ddrold on h.arirg at 56 oC for I ht. (Ad.n sr d/. l9?9) d.61.$ist.nce is.nh.nced in thc Dcs.ne of monov.lent bul nol ol divrld @tiom The ifl36 arc also rdistrnl lo idcriv.rion bv o.Eanic solvents, do\acholar., photodyMic itaclivalion $d qr.re of pE thcv "' m.ximally snblc betwee. pll 6 and 9 (MoF.ran ind Adat, 1977) Iormaldchvde disinf'ction ir .tr div. in d€totlns rhc infdivity of rvis ddoviG d.n *h.. p'ed in tludv (Ide l9?9) Since nolyncrisaion to th. inaclive t.raaom.ldehvd. can occor.t low t'mperdors,
TrlL l$ Or5a.L. dArbr ^dd.'l|s Gl!.Dl LcllidE dyEqd.@tr) 0-!2) Quil Broml'irL (Ql) Ilrdqcicrii! $rrd@0Do GrrlD n ftlktlldrb8bBnriior|B) cflpllr EgCDtq SYid@.@O Adol.d ed st@ (196)
Page 1 and 2: OBSERVATIONS ON CAUSATIVD AGENT(S)
Page 3 and 4: "M sooD&{!B N|,lr97
Page 5: ,/2 ./. -t4 In tlrc tnme of God. ll
Page 8 and 9: ACKNOIYLEDGEMENTS "Pro(hrm in th. n
Page 10 and 11: AC(NOWLEDGEMENTE LIST O[ TABLES LIS
Page 12 and 13: Cell cuhur. inocul io. PriMry viNr
Page 14 and 15: 5. RJSULTS AND DISCUSSION coNclUsto
Page 16 and 17: EXPERfMENT TNL VIll S€tuo aitibod
Page 18 and 19: T N TRoDUc7IoN
Page 20 and 21: nds in 0ocks is th€ onty signilio
Page 22 and 23: R E v T E w o F L t T E R A T U R E
Page 24 and 25: highly d.trs I'otlrry ar@s in the i
Page 26 and 27: A l.orFdc Poi$n.: h) Chlorin.red hy
Page 28 and 29: Oc@ny (Vop, | 989) Tl'.ic qinr 6nro
Page 30 and 31: pig or rit. IndnEr ha.m.gsluri.0rio
Page 32 and 33: Mrner g, z/ (1989) iiocutded lh. su
Page 34 and 35: virilotigin, perhaps an adenovirurw
Page 36 and 37: (AGPTT H.sn dr dl, t994) whit. d.re
Page 38 and 39: Trbl. l.t S.npd.l.!e or Ad.ldids Nu
Page 40 and 41: Th.s vinoN ae rcid sllblc (Hamparia
Page 44 and 45: Trbl. Lar S.relogi..l .l.ici6..tio.
Page 46 and 47: dnibodi., are rising in lield cas6o
Page 48 and 49: nun'ler ot polypenrid. bands derib.
Page 50 and 51: Phosphokinls. ocrivity lssi.lcd wil
Page 52 and 53: sigm. Th.dis4e, whichcanbeqpe mcn$l
Page 54 and 55: nrrenar oibody vitu! wc i$hr.d fron
Page 56 and 57: Th. oain problcm with any sologidl
Page 58 and 59: obwed ihar AAv-polypaptidg could b.
Page 60 and 61: M A T E R I A L s A N D M E T H o D
Page 62 and 63: lrydld.tot tc.: Th. $dlr vq!.odn d
Page 64 and 65: Infation ofchi.k Th. experim.nht ch
Page 66 and 67: lhe monolaytr (3-5 diF), ihc 06ks w
Page 68 and 69: ..diftg.d d 90,00 X Sb. 2. hor A ti
Page 70 and 71: Trta |lLr. lr..b.d.t D.Ln h. r.*. .
Page 72 and 73: EXPLR]MF.NT.IV BIOI,OCICAL CHAMCTOR
Page 74 and 75: ^\]\ M.di.r tgg Inleriv. Doe (EIDq)
Page 76 and 77: nin Th.r. cells ud. slored.t -20'C
Page 78 and 79: EXPI;RIMLNT V CHARACTI]RIZATION OF
Page 80 and 81: El.ctrophoreb vs 6icd olt chhc. i.
Page 82 and 83: @ ainjr8 l./r gchriq dd inob.t.d fo
Page 84 and 85: ofPSS .nd srored at -70oC. Pr.&nce
Page 86 and 87: T.bb V[.], Etpdindtd d6ltr ofv.ccbr
Page 88 and 89: I;XPERIMENI.VlII A DOT IMMUNOBINDIN
Page 90 and 91: R EIUL7I A No o Isc u saIo N
Page 92 and 93:
lrig.l, ll.Nrtor!chiclcn dl.d (72 l
Page 94 and 95:
Fi8.3, Int.m.l or8.r of (hi.kdr,dl.
Page 96 and 97:
ig.5. 8!6rotl.rbri.nBlronHPSig.ntin
Page 98 and 99:
Fig. ?. Photodlcrcghpt! of h.rrr rl
Page 100 and 101:
f.bL l-t. In nw ,a.l In utuo Ch.r..
Page 102 and 103:
24-48hK Absene ofhdctialgo*lh indic
Page 104 and 105:
Rdlts ofrhis srudy ee ongodt wirh r
Page 106 and 107:
lndlr.d nuorcsc.trl rntibody (IFA)
Page 108 and 109:
PURIFICATION OFTIIE HPS VIRUS men.e
Page 110 and 111:
da'dy of l.3s tnto|f i' c|O. noF€
Page 112 and 113:
T.bL tl!l. Mo.t lit h DFiL. cti.t .
Page 114 and 115:
Pithogmic in rni! nudy lhould ody b
Page 116 and 117:
T.bl. w-r. Ell6t ol C.tl Cutrrn psh
Page 118 and 119:
The anrier., ftis.d agrinsl purifi.
Page 120 and 121:
T.bb rv.3. fttldl .rd Cr.r&.t Cr.st
Page 122 and 123:
T.hL lV-4. Virur n.uhlttiio. suhi S
Page 124 and 125:
EXPE]IIMLNI.V CII RACTERIZATTON OF
Page 126 and 127:
SDSPACE shorira con0uison oa l l *r
Page 128 and 129:
Fis -Va rlso indiqt. that in &mtyp
Page 130 and 131:
a ibodt in 6 erun of low iite. The
Page 132 and 133:
Thc resulls ofSDS-PAOE ds re{@Gd ll
Page 134 and 135:
Trbl. Vll-|. S.tun a!.ibodid rg.inr
Page 136 and 137:
s 225 175 125 tm 25 :i--.'' i,; \\
Page 138 and 139:
T.hl. vl|,3. 0oir.hrllcng. Mort.lly
Page 140 and 141:
Tlblc Ml,4 indic.t6 lhd lhc pdort?c
Page 142 and 143:
I chdt.ng. t.ot.r.. Fls.Vll,3. trcr
Page 144 and 145:
'l rbli Vlll. l. Scnrilivily ThBrho
Page 146 and 147:
Tabh. VIII. 2. S.nlitivhy threlltol
Page 148 and 149:
T.blq Vlll. a. D.lalion oa vinl .nt
Page 150 and 151:
n g.tive re;ons Althoush a dositomd
Page 152 and 153:
SUMMARY llydropericarditrm lrndr6oe
Page 154 and 155:
c o N c L U s I o N s
Page 156 and 157:
R E c o n M E N D A 7 I o t I
Page 158 and 159:
L T 7 E R A 7URE c I7ED
Page 160 and 161:
Arhr,s,s z idulMI xt'r ' lrez. Risr
Page 162 and 163:
Cool.lK.A l9t2 Avi,nl|.miGiLturbnor
Page 164 and 165:
Gim,TM,DJ Kil!andsH xkva le?6 ^prli
Page 166 and 167:
Mirtrtrood, F, RU. Sh,r,id, Mz Ktun
Page 168 and 169:
ftrcifl, H 6 1156 n?ii! of ldctuida
Page 170:
v.D Ecr, J.H H . ll.C DiErsr, rA M
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HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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