HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

pig or rit. IndnEr ha.m.gsluri.0rio. (lrtA) rcsr *s f@nd ro h. rapid and sif,pl. dhod for
quanl anv. analysis of anribodi* 0gainsr Hls/AD aScnt tt Ms iecomm.ndcd rhal dis
pro4durc mi8ht be used lor scr.eninS of flocks in .pidemiotoridl sltrdics ofHpS/AD and for
.!€luainglhe innunc surls olchicks (R.hmrn.ral, 1989)
f,fietotVi.usV cirs on gPS
Vaccinario. !g.in$ Newcslle disdse (ND), inaeious bonchitis 0B), infectious
bur$l disas (lBD), .Bg drop sy.drooc (EDS) lnd rmvito! did not h.lp ii minimirnS o,
6nrolofHPs(Jal,le,1933)Thcchic*rv.ein'redwirhtBovirusv!@in.3rrinDTsdid
lor wnhstood a ch.lle.g. of500 LD, of lhe hotated virus .nd 80% ofrhc chd o8ed bi.ds
dien showirg rypical ldions of lhediss. (KhdRoj0 erat, 1933).
Expqimdts 6nducr.d by difercnl woikeG indiqr. rhll tBD vr@idrion do6 nor
p.orcat th. btr& agiiBt H?s upon exp.im.nialty chdteng. Muns sr ,/ (1939) iho
repond rhlr IBDV va@inar.d broilq chi.k.ns kre ndr ptorecr ed ,gainsl th. Hps
lrbonlory ll6t Sylr.nr
C.ll cuhord rd chick.n dnbry$ hav. h6 rscd fot the istuioi of Hps agerr
( ldb, I 988 ). C h€ema er 41. ( I 983) inoculared 8 d.y-old dcvetopins chicke. ehbryos rnroD gh
th. choiodllrntoic n mb6nc (CAM) atrd yotk ec {YS) butcs wilh tivs homogod. Mosr
oa rhc cnbryos inoal.r.d via CAM died wnhin 4 ro 6 days posl-inocul.rion *lib tn o@
mbryo Inoculared rhrough the Ys r6urosuryived. Lder.7 ind lo days old cnbryonarsd e88!
serc inocuhtcd rhrouCh cnori@llaiioic sc (CAS) lnd yS oul.s. Th. r.sutrs o6lain.n in lO
days ord embryomr.d.gge ws. rot.nouESiig a conp!rcd ro rhe Butt! obefl.d in 7_
day-old.mbryo@red cegs The mal.i.l harc&d from fie chorioallnroic fluid ofrh.
i.f€ctdd embryos was anrntuSed al 4oo0 xg tor l0 ninures rnd iijecred to de su$eptibtc
h..lrh, hroil€r chick. &tort@uiy Typiol taioD of HpS luch d E6ric Dd
hr.hoiihaeic aoci in livc., n.phrirt and nuid in p.riordilt ec w. obw.d in thr
inoculared bnds 6haw6jr, 1933)