HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

@m@n lor lhe pu.ii€rion otlinl DNA Th. firslpio..duE imlud€ culdvadon ofvitos in
approprial. @ll cuhures, lysh of infded cell! vith sdiuo dodecyl 3ulph,t. (SDS), phenol
cxrncrion of prorein, and fin.lty praipndion of th. DNA Thc oth.r pro*due involvas virus
purilorioD by ultdEntritu8anon (cscl de6iy s.&lidD, Phool dutcrion of prot.in .od
viralDNA pr€cipiition E!.h proccdurc hu ils own advettgca and disadvanhgcs. Thc l.tt.r
deib.d proc.dua is nol .s onv.nid for lh. punidioi ol rir.l DNA fton di{crot
!.mples or f6r sm.llsl. prcp@tion of non-hb€I.! DNA, .r it requir.s rhe addhionil wo*
ofhb.linS md ultftenlritug ion.
Ernytral(199Dlscdnin.eltiorionendonucl.isesind$udi'd24AustralianFAvs
olBdotypc grdp E 6 d.nn d by Z!.t dd Kisarv (1984b) F@r oa$e en vm's (H'!'III
8d,HI, &61 and /t oRI) geneded sinil& DNA fragmen$ while th. otliet tN' (APdl, Drul
Hp.I, Stpl a'xt KPnlt lahonia (m flnhd distincl 86up! On' o'lhcs grclps @tsisrt or
16 virulcd FAV9 a$lattn fton @lc IsH (htpdituLnD *hiL rh' othe' group consists of
.i!ht, nild or non-virule fAVs
Zhsg md Nagdj. (l9s9) lsd six retridion 'ndoruclM ('g' rr' F'oRl' Hin'llrl
Hha 1, Xho | $d EMAI) atu obw.d marked .L.va8e Ptlletn
diierocB dong rhr*
sologicrlly indisringuislttbl. viru*s oravirn .demvitus SrouP l! spl'nooesrl, vid!
Th. stoup III .viin admviros: $c EDS isl'rs Me sludi'd bv using Btncnon
.ndo.ucloar* Niie chic*.n virulca holat.d liom llt Unit.d Ki'sdon (UK) 'nd DclSium
sec id.nical Howwd, thre duct i$hrd Fon th. U( produc€d did4nt fragndc from
thal of chioken isol.te! An Auslt.li.n chjcken isol.tc w8 llso difio'ent ton the EuropeD
isolate oodd .t,l , r988)
VIRAL
'ROTEINS:
Th. stN.tural polrt+dda of ad.novitus have b"t $udi'd hv oev workes
Thse ht! bd somc disasr@dr is to lbc Polvpoprid' cont'ols ofld'novitus padicl's Th'