HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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lndlr.d nuorcsc.trl rntibody (IFA) rat. Fluoesccrc. B li6h€d ro fte nuclei of i'r6tcd ellt S.v4.l sall pinpoint indusions wse ioitia[y ddd!bl.. At th. later sttg.s oa infection rh*e cnltrg.d dd inclo.sed in rumbq until scvenl 1.r8. id.g!l.r ar.8 of ouor*nl rntig€n w.r. obs.Ped in inadcd nuclei Anlis.n sF o0cn @@nlriLd t@nd th..uclar pqiph.ry' Duc lo rh. lsynchronour nallr. ol th. infelion, nu.l.i at diffcrcnt sl.g* wer. onen s.en in lhc srdc ohure Oift rdt 3uges arc slo$ i. FiB 9 Pr6@t infomalion i3 ale in .8r..B.nl wnh 1ne inrlinss of KawaoE tt zl ( 1964), Fldly (19?s) and Relter ., al (19?9) who d*ib.d vlrious sEg's of inclusion bodv fouion hy ad.novirus ASrr gcl pr.cipit.lion (aCP) l.!l Th. AGP t.st d.mNtRl.d slon8 line ol iddnv bNan rh' vinl 'nli84 0nd lnismn ro AAV *rot pc4 at 48 hG No line oi iddthv wls d'mnslritcd be{wd lhc vir.l Mrig.n and tr.sativc antiserun, sutsg.{ing $.t lhe i$laled viN shred srclp{Pecifrc od@opdd antisc., *ith gdp I rtan .ddovios Obed.tions of $i3 siudy @dobot.E llt findin$ of Malherbe ind Strickland_ Cholml.t (t9s3) who {gg.st.d thar virutinduc.d dotphologic chans6 i'volwd en' d.e.e of c€ll di$rBaniatiot\ .ilh€r in $e form of de{rudion and di$onion of cell componcnts, or in rhc fom or dkplrcedcm of lhcle bv inttusion of n@g'nelic naierials El@ron miqospy 6 dilcd Nbrlc d.toDkrl chxa.!, bu mr' onmonlv cdl al'uc r*hniquc h .mploycd lor thc del.ction ol vitus plnicl's l[mselves and nol rh'changes rhev indu@ Th. loolion .nd 3htp. of lpdific ..ti8dic $bst'@s @al'd bv immuno_ ,luorsc.n6 undd the llr6viol.l nidotcope mlv in I ene indicatiig nolpholoeial ahdatioNint c.lt Cowdry (1914) onis *pltin d lh.t virus indlc'd inclusions mv or ntv not onl0in vitu! plnicl6 TIE crlop.thic.ffd! olddovios M'ot 8 ' ol" b' Gldil' coitued wnh thos ofothq viruss
PhotomicmgrrDh ofnuore$enc.,linir.d ro the nnct.i ot HPS 4.ni inLcr.d lEp.tocyr.r, rhowlng inctuion bodt.t
Page 1 and 2:
OBSERVATIONS ON CAUSATIVD AGENT(S)
Page 3 and 4:
"M sooD&{!B N|,lr97
Page 5:
,/2 ./. -t4 In tlrc tnme of God. ll
Page 8 and 9:
ACKNOIYLEDGEMENTS "Pro(hrm in th. n
Page 10 and 11:
AC(NOWLEDGEMENTE LIST O[ TABLES LIS
Page 12 and 13:
Cell cuhur. inocul io. PriMry viNr
Page 14 and 15:
5. RJSULTS AND DISCUSSION coNclUsto
Page 16 and 17:
EXPERfMENT TNL VIll S€tuo aitibod
Page 18 and 19:
T N TRoDUc7IoN
Page 20 and 21:
nds in 0ocks is th€ onty signilio
Page 22 and 23:
R E v T E w o F L t T E R A T U R E
Page 24 and 25:
highly d.trs I'otlrry ar@s in the i
Page 26 and 27:
A l.orFdc Poi$n.: h) Chlorin.red hy
Page 28 and 29:
Oc@ny (Vop, | 989) Tl'.ic qinr 6nro
Page 30 and 31:
pig or rit. IndnEr ha.m.gsluri.0rio
Page 32 and 33:
Mrner g, z/ (1989) iiocutded lh. su
Page 34 and 35:
virilotigin, perhaps an adenovirurw
Page 36 and 37:
(AGPTT H.sn dr dl, t994) whit. d.re
Page 38 and 39:
Trbl. l.t S.npd.l.!e or Ad.ldids Nu
Page 40 and 41:
Th.s vinoN ae rcid sllblc (Hamparia
Page 42 and 43:
Tte viruss sharc a 6mnon goup antis
Page 44 and 45:
Trbl. Lar S.relogi..l .l.ici6..tio.
Page 46 and 47:
dnibodi., are rising in lield cas6o
Page 48 and 49:
nun'ler ot polypenrid. bands derib.
Page 50 and 51:
Phosphokinls. ocrivity lssi.lcd wil
Page 52 and 53:
sigm. Th.dis4e, whichcanbeqpe mcn$l
Page 54 and 55:
nrrenar oibody vitu! wc i$hr.d fron
Page 56 and 57: Th. oain problcm with any sologidl
Page 58 and 59: obwed ihar AAv-polypaptidg could b.
Page 60 and 61: M A T E R I A L s A N D M E T H o D
Page 62 and 63: lrydld.tot tc.: Th. $dlr vq!.odn d
Page 64 and 65: Infation ofchi.k Th. experim.nht ch
Page 66 and 67: lhe monolaytr (3-5 diF), ihc 06ks w
Page 68 and 69: ..diftg.d d 90,00 X Sb. 2. hor A ti
Page 70 and 71: Trta |lLr. lr..b.d.t D.Ln h. r.*. .
Page 72 and 73: EXPLR]MF.NT.IV BIOI,OCICAL CHAMCTOR
Page 74 and 75: ^\]\ M.di.r tgg Inleriv. Doe (EIDq)
Page 76 and 77: nin Th.r. cells ud. slored.t -20'C
Page 78 and 79: EXPI;RIMLNT V CHARACTI]RIZATION OF
Page 80 and 81: El.ctrophoreb vs 6icd olt chhc. i.
Page 82 and 83: @ ainjr8 l./r gchriq dd inob.t.d fo
Page 84 and 85: ofPSS .nd srored at -70oC. Pr.&nce
Page 86 and 87: T.bb V[.], Etpdindtd d6ltr ofv.ccbr
Page 88 and 89: I;XPERIMENI.VlII A DOT IMMUNOBINDIN
Page 90 and 91: R EIUL7I A No o Isc u saIo N
Page 92 and 93: lrig.l, ll.Nrtor!chiclcn dl.d (72 l
Page 94 and 95: Fi8.3, Int.m.l or8.r of (hi.kdr,dl.
Page 96 and 97: ig.5. 8!6rotl.rbri.nBlronHPSig.ntin
Page 98 and 99: Fig. ?. Photodlcrcghpt! of h.rrr rl
Page 100 and 101: f.bL l-t. In nw ,a.l In utuo Ch.r..
Page 102 and 103: 24-48hK Absene ofhdctialgo*lh indic
Page 104 and 105: Rdlts ofrhis srudy ee ongodt wirh r
Page 108 and 109: PURIFICATION OFTIIE HPS VIRUS men.e
Page 110 and 111: da'dy of l.3s tnto|f i' c|O. noF€
Page 112 and 113: T.bL tl!l. Mo.t lit h DFiL. cti.t .
Page 114 and 115: Pithogmic in rni! nudy lhould ody b
Page 116 and 117: T.bl. w-r. Ell6t ol C.tl Cutrrn psh
Page 118 and 119: The anrier., ftis.d agrinsl purifi.
Page 120 and 121: T.bb rv.3. fttldl .rd Cr.r&.t Cr.st
Page 122 and 123: T.hL lV-4. Virur n.uhlttiio. suhi S
Page 124 and 125: EXPE]IIMLNI.V CII RACTERIZATTON OF
Page 126 and 127: SDSPACE shorira con0uison oa l l *r
Page 128 and 129: Fis -Va rlso indiqt. that in &mtyp
Page 130 and 131: a ibodt in 6 erun of low iite. The
Page 132 and 133: Thc resulls ofSDS-PAOE ds re{@Gd ll
Page 134 and 135: Trbl. Vll-|. S.tun a!.ibodid rg.inr
Page 136 and 137: s 225 175 125 tm 25 :i--.'' i,; \\
Page 138 and 139: T.hl. vl|,3. 0oir.hrllcng. Mort.lly
Page 140 and 141: Tlblc Ml,4 indic.t6 lhd lhc pdort?c
Page 142 and 143: I chdt.ng. t.ot.r.. Fls.Vll,3. trcr
Page 144 and 145: 'l rbli Vlll. l. Scnrilivily ThBrho
Page 146 and 147: Tabh. VIII. 2. S.nlitivhy threlltol
Page 148 and 149: T.blq Vlll. a. D.lalion oa vinl .nt
Page 150 and 151: n g.tive re;ons Althoush a dositomd
Page 152 and 153: SUMMARY llydropericarditrm lrndr6oe
Page 154 and 155: c o N c L U s I o N s
Page 156 and 157:
R E c o n M E N D A 7 I o t I
Page 158 and 159:
L T 7 E R A 7URE c I7ED
Page 160 and 161:
Arhr,s,s z idulMI xt'r ' lrez. Risr
Page 162 and 163:
Cool.lK.A l9t2 Avi,nl|.miGiLturbnor
Page 164 and 165:
Gim,TM,DJ Kil!andsH xkva le?6 ^prli
Page 166 and 167:
Mirtrtrood, F, RU. Sh,r,id, Mz Ktun
Page 168 and 169:
ftrcifl, H 6 1156 n?ii! of ldctuida
Page 170:
v.D Ecr, J.H H . ll.C DiErsr, rA M
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