HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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lrydld.tot tc.: Th. $dlr vq!.odn d o bd!., trF d b!.di ditu F!q!!d t!6 M/!i 3B cti*., Pdvn. Umibd, rjrd!.d, p*htu. r@ i|ohro ob!i!.d Ad inGdcd cuot@! n d .lifhrd .or!€ in, Fivd. d tov.E d FIn, e@; ,ct y d.gDdc Lbontod.q fid ldqdrg th. ortEt of tjrdroFiq.dih.r!dre@(n@: rdrn&d. Ml!itr., r.h.i!, F.tuld.d .d X!*ta didi.4 !d lt .ti.r.{. ded 6. e..ir6r.l mtr G. !t d in dE Fgr iNdartidt andi-,
ISOLATION AND PNOTACATTON OF EPS AGENT Sou.c. of vitut ttrd it! prop.grtlonr Fivc isolats of AAV wde r@v.rd from liv.r homog.. e or ihe bnds died of HPS lolrowing srr.drd p.e.dur* (Nadm d/ at., | 995). l$la|e A & B vcrc broild i$lrt.s, C & D w. bt4ds i.ol 6 ald E wls prororrp. ishc A 20./q hoooscml. fron liE v.r prcpar.d in phosphlre buF.r $lin (pH 7 2) wirh lddirion of p.nicillin sodiun (1q000 | U/Dl)r ltEplomyci. slphal. (2 hs/ml)r s.nllmicin suhhde (1.0 mglml), t.namycin suhh.t. (065 ns/ml)i and mpholdicin B (002 ng/nrli S.nne, 1939). The homo8oab w!! c.ntitugcd it 3,000 rp6 aor 20 ninutes in ! rofiigdatd c.idtuge (4 lo'C) to editunl li$u. d.bis rnd my b.ct.ria. Thc euhi.s $pendbnt *as .*prqlly renov.d .nd 6lr6ed rhrouAh 0.,15 ..d 0 2 mic.on 6lG. The fiXrat. s3 funho @rifuged a125,000 rpo (30,000 x B) lor m miorB Th. p.lld wN esp.ndcd i. PBS (pH ?.2) aDd fhcred rh'ough lm nm filla. ro.tlo* only $. p.ss€ oatitus Th. 6lt rc wr! lrd for ielllioo ofvirus lhrou8h lhc i,nlowing proc.du6: lll C.ll cultur. inodlarion A total or l0 clinically nomd broild cliiclo (Hubbd x Hubbed) $. .9. of 25 drys ws. obtdned frcn . lo@l hlrch.ry. Th. chictr w.rc id€dined by winS bdding ind divid.d i.ro rhr@ ercup3 ach oBining of id broil6s. Aft.r rt:trfd 10 1fi. isol,tion nnir .f rhe Anio.l Healtti Lib., Aninil Scid€ l6litur., Nliionrl Agdoltunl R@rch Cotrq lslanab.d, individu.l sa oflh. ohick v@ indyz.d by aed s.l l9?3) !o dctcmiie preiioelarion antibodi.s pr4iDitrtion Lst (Ctowl.,
Page 1 and 2:
OBSERVATIONS ON CAUSATIVD AGENT(S)
Page 3 and 4:
"M sooD&{!B N|,lr97
Page 5:
,/2 ./. -t4 In tlrc tnme of God. ll
Page 8 and 9:
ACKNOIYLEDGEMENTS "Pro(hrm in th. n
Page 10 and 11:
AC(NOWLEDGEMENTE LIST O[ TABLES LIS
Page 12 and 13: Cell cuhur. inocul io. PriMry viNr
Page 14 and 15: 5. RJSULTS AND DISCUSSION coNclUsto
Page 16 and 17: EXPERfMENT TNL VIll S€tuo aitibod
Page 18 and 19: T N TRoDUc7IoN
Page 20 and 21: nds in 0ocks is th€ onty signilio
Page 22 and 23: R E v T E w o F L t T E R A T U R E
Page 24 and 25: highly d.trs I'otlrry ar@s in the i
Page 26 and 27: A l.orFdc Poi$n.: h) Chlorin.red hy
Page 28 and 29: Oc@ny (Vop, | 989) Tl'.ic qinr 6nro
Page 30 and 31: pig or rit. IndnEr ha.m.gsluri.0rio
Page 32 and 33: Mrner g, z/ (1989) iiocutded lh. su
Page 34 and 35: virilotigin, perhaps an adenovirurw
Page 36 and 37: (AGPTT H.sn dr dl, t994) whit. d.re
Page 38 and 39: Trbl. l.t S.npd.l.!e or Ad.ldids Nu
Page 40 and 41: Th.s vinoN ae rcid sllblc (Hamparia
Page 42 and 43: Tte viruss sharc a 6mnon goup antis
Page 44 and 45: Trbl. Lar S.relogi..l .l.ici6..tio.
Page 46 and 47: dnibodi., are rising in lield cas6o
Page 48 and 49: nun'ler ot polypenrid. bands derib.
Page 50 and 51: Phosphokinls. ocrivity lssi.lcd wil
Page 52 and 53: sigm. Th.dis4e, whichcanbeqpe mcn$l
Page 54 and 55: nrrenar oibody vitu! wc i$hr.d fron
Page 56 and 57: Th. oain problcm with any sologidl
Page 58 and 59: obwed ihar AAv-polypaptidg could b.
Page 60 and 61: M A T E R I A L s A N D M E T H o D
Page 64 and 65: Infation ofchi.k Th. experim.nht ch
Page 66 and 67: lhe monolaytr (3-5 diF), ihc 06ks w
Page 68 and 69: ..diftg.d d 90,00 X Sb. 2. hor A ti
Page 70 and 71: Trta |lLr. lr..b.d.t D.Ln h. r.*. .
Page 72 and 73: EXPLR]MF.NT.IV BIOI,OCICAL CHAMCTOR
Page 74 and 75: ^\]\ M.di.r tgg Inleriv. Doe (EIDq)
Page 76 and 77: nin Th.r. cells ud. slored.t -20'C
Page 78 and 79: EXPI;RIMLNT V CHARACTI]RIZATION OF
Page 80 and 81: El.ctrophoreb vs 6icd olt chhc. i.
Page 82 and 83: @ ainjr8 l./r gchriq dd inob.t.d fo
Page 84 and 85: ofPSS .nd srored at -70oC. Pr.&nce
Page 86 and 87: T.bb V[.], Etpdindtd d6ltr ofv.ccbr
Page 88 and 89: I;XPERIMENI.VlII A DOT IMMUNOBINDIN
Page 90 and 91: R EIUL7I A No o Isc u saIo N
Page 92 and 93: lrig.l, ll.Nrtor!chiclcn dl.d (72 l
Page 94 and 95: Fi8.3, Int.m.l or8.r of (hi.kdr,dl.
Page 96 and 97: ig.5. 8!6rotl.rbri.nBlronHPSig.ntin
Page 98 and 99: Fig. ?. Photodlcrcghpt! of h.rrr rl
Page 100 and 101: f.bL l-t. In nw ,a.l In utuo Ch.r..
Page 102 and 103: 24-48hK Absene ofhdctialgo*lh indic
Page 104 and 105: Rdlts ofrhis srudy ee ongodt wirh r
Page 106 and 107: lndlr.d nuorcsc.trl rntibody (IFA)
Page 108 and 109: PURIFICATION OFTIIE HPS VIRUS men.e
Page 110 and 111: da'dy of l.3s tnto|f i' c|O. noF€
Page 112 and 113:
T.bL tl!l. Mo.t lit h DFiL. cti.t .
Page 114 and 115:
Pithogmic in rni! nudy lhould ody b
Page 116 and 117:
T.bl. w-r. Ell6t ol C.tl Cutrrn psh
Page 118 and 119:
The anrier., ftis.d agrinsl purifi.
Page 120 and 121:
T.bb rv.3. fttldl .rd Cr.r&.t Cr.st
Page 122 and 123:
T.hL lV-4. Virur n.uhlttiio. suhi S
Page 124 and 125:
EXPE]IIMLNI.V CII RACTERIZATTON OF
Page 126 and 127:
SDSPACE shorira con0uison oa l l *r
Page 128 and 129:
Fis -Va rlso indiqt. that in &mtyp
Page 130 and 131:
a ibodt in 6 erun of low iite. The
Page 132 and 133:
Thc resulls ofSDS-PAOE ds re{@Gd ll
Page 134 and 135:
Trbl. Vll-|. S.tun a!.ibodid rg.inr
Page 136 and 137:
s 225 175 125 tm 25 :i--.'' i,; \\
Page 138 and 139:
T.hl. vl|,3. 0oir.hrllcng. Mort.lly
Page 140 and 141:
Tlblc Ml,4 indic.t6 lhd lhc pdort?c
Page 142 and 143:
I chdt.ng. t.ot.r.. Fls.Vll,3. trcr
Page 144 and 145:
'l rbli Vlll. l. Scnrilivily ThBrho
Page 146 and 147:
Tabh. VIII. 2. S.nlitivhy threlltol
Page 148 and 149:
T.blq Vlll. a. D.lalion oa vinl .nt
Page 150 and 151:
n g.tive re;ons Althoush a dositomd
Page 152 and 153:
SUMMARY llydropericarditrm lrndr6oe
Page 154 and 155:
c o N c L U s I o N s
Page 156 and 157:
R E c o n M E N D A 7 I o t I
Page 158 and 159:
L T 7 E R A 7URE c I7ED
Page 160 and 161:
Arhr,s,s z idulMI xt'r ' lrez. Risr
Page 162 and 163:
Cool.lK.A l9t2 Avi,nl|.miGiLturbnor
Page 164 and 165:
Gim,TM,DJ Kil!andsH xkva le?6 ^prli
Page 166 and 167:
Mirtrtrood, F, RU. Sh,r,id, Mz Ktun
Page 168 and 169:
ftrcifl, H 6 1156 n?ii! of ldctuida
Page 170:
v.D Ecr, J.H H . ll.C DiErsr, rA M
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HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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