HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

a ibodt in 6 erun of low iite. The techniquc cven allovs $c recognitio n of prol'ins thal
are rot nse$sily suda€ sposed {Bld$r d nl., 1984)
Keepirs i. view lhe reqnirem.nt! of sm'ller amounu of tntis'n and antibodv th'
wlB M ued i. th. prent slndv to obdn infomtion on lh' !'olosiol rdaledm$ of
pARC-l with othq AAv tvpei rnd lo d.t.dim wherfis @m rndbodict dn4ted a3linst
Droreins of AAv typc w.r. tyte sp.cifrc
These dl)erin.nlsdemonstraled rhar PARC_l isolare and AAV serolvpe'4 efl ses arc
anrigelicilly clos.ly relared Serotvpe_9 lho shr'd some sntigens iid *ems to be le$
clo$ly relsr.d lmnDnoblols ol orlu AAV s.iotvp6 indided $e trc!'nce of manv @mon
bnnds rsali.g &roloaic linildti.: lhona tha | ! AAv mtvpd
As thc anlkcra dcrins with th. blol 6 onsinad fron AAV PARCr' thos'
prolei.s wcr. only r@Snized wbi.h ver. d.rccr.ble bv $e imnunog'nic lniibodB prd'nl
inlhe serum Th.r.mighthcsoneoths*rolvPcspecificanribodi4inolhdlvpeoftheavian
rderovidses Sinc. out obj@rive ws to idodry rhe @6mon immunogcn'c annse's amons
I I *rolypci onty hrpdimmnm s! fiom AAV-PARC-I inool'r'd bids w' us'd
Tt immud$rd sd,q indi@tql rll!1 d le! fre or th€ mjor I'rot'i
aI il* srtpas (Fi& \4-a). sinn( $!di6 mmpding AAv sorrp6 vith HPS aasild Aev
mre nor inden rkor in I he liast The innftd ol |rolvpQl
ide baids lisiblc in AAV isolat' were 7
Due to tlE pGs.nce of vtnous lnme6 and dincn iheft is 0lwdvs i fak ch!'ce or setns more
bfl& ifdifrd.nr m.lho& loi pot!.pide dhruption sG uld A@e it tu als posibL rh't sm
orde epitop6 wlici did d.ad vitb lhc mtiGt mier h.wb€n d.iatu"d dujng SDSPACE
and lhs d.n.rurcd po&is nighr h.s d l@sni2td rtE 54m ditodi6 (Btmdtc, 193')
More $!di.s dDuld b..rempt d, in lhis rcga.d, bv chmgine fie dderydt3, d$slink6 od 3'l
@n@rrarion trmunoblol oalFis @edcd rhal fie pob?eptides ll dnd V ol HPS 4soci ed
AAY wer. slrcngly related b rhe mo MW proteins of olhd €leven *'otvpq of AAv The
Flypepid.
vl olislat PARc-l dMgly rad.d to $e lrylgimurc mn of HlSv wh€s
rlE $tu s'n ed.d p@ly wnn 24 (D po|.jn of s@gTc9 This itv indiane difecE in
imnogdi.ity moDg lhE ruo.