HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

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The anrier., ftis.d agrinsl purifi.d HpS ag.nl in chict., did nol show .ny c,o!, r@cr'oi asa,nn organNms such .r iif.crior bu^aldhcse vios (lBDv), chick maemid agem (CM), N.wqlde dis4 vatu (NDv), @virur .nd iiterio$ broiciiln vios (tBV) N6G of rhose vitu*s werc isoldl.d fron iifEled bkds indicaling rhat only rhe HISV prodfted Angara dis.ls lBaoN. Il hax how.v.r,6c indicaled thd s@ qnid studies havd sug86l.d enc rc|. ol IBDV or r.ovios in podu.ing HPS by AAV {McDougalt & percij, t97a, M.len^n .t al , t974) An i@eh.drl shrp. of $c HPS lior simil.. to .viNn id..ovirus, w.s obseoed ulder lhc m'cioeopc in the grids p.epar.d 6on lhe luorose gndie.t puritied preparalions (Fis Iv l) h may b. renriond d'i! HPSV ssp.Nion did ndr hlr any odaminaton wirh TIE i'Eulation oflhe puified viru3 @s.d lypidl l6ioB ofl{PS wnh hiBh modrlir, in sr$.plibb bnds. Nempry dlmimtion old€ad and modid cnickds rcv. .d pale ur disloured liver that w.s slishdy rwoller al{l |riabl. lnd had necroric loci ofvariohle siz.s Protu*t@umul ion off,uid in !.risrdid ec wa ob*r.d ir 30 pq eni of$. dod bnds The kidncys ofinl@ted birds wer. pale, md cnlarged wirh disrctrd.d lubules Microscdpic .min.lion ol snaB fton inldtcd tivc6 rcval.d b.ephilic int..nucldr inclusioo bodi.s ln rhe virus chlracr.daion nudi.,, heat |ran.d (6o'C aor r0 nin) vitos did rcr prcduc. fo'i.liry in the sscepr ibl. unds orbl.lv-l) Th. chlorotoh and crhd lr.rm.nk did nor inactivate the virus s it ered typical HIS upon inoculation into susc.pribG bnds ll @ al$ obeaed rh HPSV i.feritty wa not afecrcd !y irs lr..16. wnh dt.ctu pH of Ad.novirus .xhihn a hi8h des.e ol r4isr.n@ to a vid. rans. of physicrl a.d chemicalagenls, mos !!e sabl. on hating lo t6"C alrhough some isohtcs are inacrvared sr 5o'c (McFs.M .nd Adan, 1977) Fidis Eponr indi{r.d rh.r EDS vitu ina.crivity w., d.(rey.d o! h.lting it 0t 56"c for I hr (Ad.hriar, r9?9) Tne turt.y hodo.ihigic
Iris.lv.t. Eh.tu' Dio.o[ Il' orlll'sY piiidc (rirc no: tA;120 'lxys DDnnn'rhriotr;{hred:?r4t-r915 tl,?0,000\r,rgnifiqnion).
Page 1 and 2:
OBSERVATIONS ON CAUSATIVD AGENT(S)
Page 3 and 4:
"M sooD&{!B N|,lr97
Page 5:
,/2 ./. -t4 In tlrc tnme of God. ll
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ACKNOIYLEDGEMENTS "Pro(hrm in th. n
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AC(NOWLEDGEMENTE LIST O[ TABLES LIS
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Cell cuhur. inocul io. PriMry viNr
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5. RJSULTS AND DISCUSSION coNclUsto
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EXPERfMENT TNL VIll S€tuo aitibod
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T N TRoDUc7IoN
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nds in 0ocks is th€ onty signilio
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R E v T E w o F L t T E R A T U R E
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highly d.trs I'otlrry ar@s in the i
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A l.orFdc Poi$n.: h) Chlorin.red hy
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Oc@ny (Vop, | 989) Tl'.ic qinr 6nro
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pig or rit. IndnEr ha.m.gsluri.0rio
Page 32 and 33:
Mrner g, z/ (1989) iiocutded lh. su
Page 34 and 35:
virilotigin, perhaps an adenovirurw
Page 36 and 37:
(AGPTT H.sn dr dl, t994) whit. d.re
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Trbl. l.t S.npd.l.!e or Ad.ldids Nu
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Th.s vinoN ae rcid sllblc (Hamparia
Page 42 and 43:
Tte viruss sharc a 6mnon goup antis
Page 44 and 45:
Trbl. Lar S.relogi..l .l.ici6..tio.
Page 46 and 47:
dnibodi., are rising in lield cas6o
Page 48 and 49:
nun'ler ot polypenrid. bands derib.
Page 50 and 51:
Phosphokinls. ocrivity lssi.lcd wil
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sigm. Th.dis4e, whichcanbeqpe mcn$l
Page 54 and 55:
nrrenar oibody vitu! wc i$hr.d fron
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Th. oain problcm with any sologidl
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obwed ihar AAv-polypaptidg could b.
Page 60 and 61:
M A T E R I A L s A N D M E T H o D
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lrydld.tot tc.: Th. $dlr vq!.odn d
Page 64 and 65:
Infation ofchi.k Th. experim.nht ch
Page 66 and 67:
lhe monolaytr (3-5 diF), ihc 06ks w
Page 68 and 69: ..diftg.d d 90,00 X Sb. 2. hor A ti
Page 70 and 71: Trta |lLr. lr..b.d.t D.Ln h. r.*. .
Page 72 and 73: EXPLR]MF.NT.IV BIOI,OCICAL CHAMCTOR
Page 74 and 75: ^\]\ M.di.r tgg Inleriv. Doe (EIDq)
Page 76 and 77: nin Th.r. cells ud. slored.t -20'C
Page 78 and 79: EXPI;RIMLNT V CHARACTI]RIZATION OF
Page 80 and 81: El.ctrophoreb vs 6icd olt chhc. i.
Page 82 and 83: @ ainjr8 l./r gchriq dd inob.t.d fo
Page 84 and 85: ofPSS .nd srored at -70oC. Pr.&nce
Page 86 and 87: T.bb V[.], Etpdindtd d6ltr ofv.ccbr
Page 88 and 89: I;XPERIMENI.VlII A DOT IMMUNOBINDIN
Page 90 and 91: R EIUL7I A No o Isc u saIo N
Page 92 and 93: lrig.l, ll.Nrtor!chiclcn dl.d (72 l
Page 94 and 95: Fi8.3, Int.m.l or8.r of (hi.kdr,dl.
Page 96 and 97: ig.5. 8!6rotl.rbri.nBlronHPSig.ntin
Page 98 and 99: Fig. ?. Photodlcrcghpt! of h.rrr rl
Page 100 and 101: f.bL l-t. In nw ,a.l In utuo Ch.r..
Page 102 and 103: 24-48hK Absene ofhdctialgo*lh indic
Page 104 and 105: Rdlts ofrhis srudy ee ongodt wirh r
Page 106 and 107: lndlr.d nuorcsc.trl rntibody (IFA)
Page 108 and 109: PURIFICATION OFTIIE HPS VIRUS men.e
Page 110 and 111: da'dy of l.3s tnto|f i' c|O. noF€
Page 112 and 113: T.bL tl!l. Mo.t lit h DFiL. cti.t .
Page 114 and 115: Pithogmic in rni! nudy lhould ody b
Page 116 and 117: T.bl. w-r. Ell6t ol C.tl Cutrrn psh
Page 120 and 121: T.bb rv.3. fttldl .rd Cr.r&.t Cr.st
Page 122 and 123: T.hL lV-4. Virur n.uhlttiio. suhi S
Page 124 and 125: EXPE]IIMLNI.V CII RACTERIZATTON OF
Page 126 and 127: SDSPACE shorira con0uison oa l l *r
Page 128 and 129: Fis -Va rlso indiqt. that in &mtyp
Page 130 and 131: a ibodt in 6 erun of low iite. The
Page 132 and 133: Thc resulls ofSDS-PAOE ds re{@Gd ll
Page 134 and 135: Trbl. Vll-|. S.tun a!.ibodid rg.inr
Page 136 and 137: s 225 175 125 tm 25 :i--.'' i,; \\
Page 138 and 139: T.hl. vl|,3. 0oir.hrllcng. Mort.lly
Page 140 and 141: Tlblc Ml,4 indic.t6 lhd lhc pdort?c
Page 142 and 143: I chdt.ng. t.ot.r.. Fls.Vll,3. trcr
Page 144 and 145: 'l rbli Vlll. l. Scnrilivily ThBrho
Page 146 and 147: Tabh. VIII. 2. S.nlitivhy threlltol
Page 148 and 149: T.blq Vlll. a. D.lalion oa vinl .nt
Page 150 and 151: n g.tive re;ons Althoush a dositomd
Page 152 and 153: SUMMARY llydropericarditrm lrndr6oe
Page 154 and 155: c o N c L U s I o N s
Page 156 and 157: R E c o n M E N D A 7 I o t I
Page 158 and 159: L T 7 E R A 7URE c I7ED
Page 160 and 161: Arhr,s,s z idulMI xt'r ' lrez. Risr
Page 162 and 163: Cool.lK.A l9t2 Avi,nl|.miGiLturbnor
Page 164 and 165: Gim,TM,DJ Kil!andsH xkva le?6 ^prli
Page 166 and 167: Mirtrtrood, F, RU. Sh,r,id, Mz Ktun
Page 168 and 169:
ftrcifl, H 6 1156 n?ii! of ldctuida
Page 170:
v.D Ecr, J.H H . ll.C DiErsr, rA M
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