HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

inabal.d lt 4 oC for 24 h.s. Thc dhq ss rllow€d io cv.po6re from rh. t.aned
preparalion Experimenbl rnd cont.ol natcidh wcrc inodlrtcd .s a sinsle dos. rale of I ml
ps chick slbolanoNly in l5-day old chi.li.nd non.lit, w.s r@rded up to 5 d.ys. Th€
vin! w.r 6osidd.i ,.NniE if qpNF b d. .rh.r @llcd in orc lo8 dd6. in
inldiliry conpden lo th. uDtral.d conlrols
Elfect of pH: The puriicd vitus ws nixcd wilh butr B ol pHr and pHtr Aner I hr of
inobltion .r l? oC, rhc $lurao wc inocuht.d 10 l5n!y old su&.prible cfick! .| $e dos
ai. of I mt pd t@ln.nt Mondny us @rdcd thqe !ffq up to 5 days Aderoscolone
log or morc in inlectivily wrs considdcd a! indicatire of &nsitivily lo lov ot high pH The
rct..ti6. of acrivity o. of lny d€@e lBs thin one log, w.s @nsidcrcd indicative of
S.n-tyDing ol th. i$lil.t Thc o.e *6y virus ndtralialion tdi wrs ploy.d in CEL cell
qltur. ro ryp. ihe i$lrt. by lsins prototyp. snlisa of l-12 sqottTs ol sronpl avid
.dqovitus tollosing tha lroc.durc deribcd a id (McFcrtn, 199!) ari.ny, 50-500
TclD! oa HIS virus w$ rdl.d l€ci6t 2lold dilurioM of AAV r.ferde sm ol l_l I Th.
test {ds pcrforoed h 96-*.ll plares cont.irring conflue.t monollyer of CEL cclls. Aner, 6
{tays iMbaion a( 37C in 5rl. CO,,lhe.nd poinl w€r. cdol.lcd .s tb. high.st dilulion of
!@n q$ing @mplqto n4k li2lnon of vi6l inrcriviry.