HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

Chapter 3
VituB, Cdt,.trd M.diun
MATENALS AND METHODS
The virus diainr uscd i. $k experihent were procured fiom the followins sources:
l. Thc Aljd Addolitu3* ws. obtaincd f.on Nrtional Vdddry S.ei@s
Laborirorier (NVSL), Io*r, USA
2. Hydop.riddilm SJndrooc 0lPS) viN isld6 w. obt.in d ftd
Vebdnary Rerarch lBti$te, l-ahorc, rnd Pouhry Res.aroh Institut., Ra*alpindi,
I li?S Vaccine s.oples {qc supplied by I\tS Biolabs, hl.6!b0d, dd
n4s v.ry'c@ (p{) Itrd , R.srlpindi, i'*isun
4. Quiil testicle (QT) ell lin. onsrDatcd from or NvsL, Iow., UsAi
vqo all lin. from Naliodl hstild. oa tL.llh
0.,rlH), Islamrbld, Plkist.n
t. Nesrriw dd p6itiv. b?simun $6 nisqd in spailic pathosd f.@ (SPF)
chickm wse Procuad iiom NVSL, low4 USA
6 Elslc 3 mininrl .sglirl nedh (E' MEM) supploeit.d wnh fet8l @lr@m
(!csiNADC, Ares, IA) and 5 mg/100 t gdtoicin $llal. (sch.ins CorPotation,
Dificrc orgrnr likc liv.r, tidneyt, 3PlM, hc!n, Iungi mu$|.s, lvmphoid orsm,!
bus. of Flbricius, lhymus, inlatin* €r.. w.r. coll.clcd aseptic.llv. froo chrck.6 in which
diss vs dpdimcntdly inJuc.d Th. El.otcd tiscs for histop nobgical dmioadon
v.c fix.n unns wiout fix.tiv.r (lopl. for60l $li@, b. 6 nui4 6ld 2% slurdrrd.hvd.
.tc ). Fo. *olosicrl {udi6, motbid livq tiscs wd. tlor.n .i$.r in srdilizcd plislic bags in
ice or in Blyc.nne durin8 tmponllion