HYDROPXRICAru)II'M SYI{DROME (ANGARA DISEASE) IN ...

The anrier., ftis.d agrinsl purifi.d HpS ag.nl in chict., did nol show .ny c,o!,
r@cr'oi asa,nn organNms such .r iif.crior bu^aldhcse vios (lBDv), chick maemid
agem (CM), N.wqlde dis4 vatu (NDv), @virur .nd iiterio$ broiciiln vios (tBV)
N6G of rhose vitu*s werc isoldl.d fron iifEled bkds indicaling rhat only rhe HISV
prodfted Angara dis.ls lBaoN. Il hax how.v.r,6c indicaled thd s@ qnid studies havd
sug86l.d enc rc|. ol IBDV or r.ovios in podu.ing HPS by AAV {McDougalt & percij,
t97a, M.len^n .t al , t974)
An i@eh.drl shrp. of $c HPS lior simil.. to .viNn id..ovirus, w.s obseoed
ulder lhc m'cioeopc in the grids p.epar.d 6on lhe luorose gndie.t puritied preparalions
(Fis Iv l) h may b. renriond d'i! HPSV ssp.Nion did ndr hlr any odaminaton wirh
TIE i'Eulation oflhe puified viru3 @s.d lypidl l6ioB ofl{PS wnh hiBh modrlir,
in sr$.plibb bnds. Nempry dlmimtion old€ad and modid cnickds rcv. .d pale ur
disloured liver that w.s slishdy rwoller al{l |riabl. lnd had necroric loci ofvariohle siz.s
Protu*t@umul ion off,uid in !.risrdid ec wa ob*r.d ir 30 pq eni of$. dod bnds
The kidncys ofinl@ted birds wer. pale, md cnlarged wirh disrctrd.d lubules Microscdpic
.min.lion ol snaB fton inldtcd tivc6 rcval.d b.ephilic int..nucldr inclusioo bodi.s
ln rhe virus chlracr.daion nudi.,, heat |ran.d (6o'C aor r0 nin) vitos did rcr
prcduc. fo'i.liry in the sscepr ibl. unds orbl.lv-l) Th. chlorotoh and crhd lr.rm.nk
did nor inactivate the virus s it ered typical HIS upon inoculation into susc.pribG bnds ll
@ al$ obeaed rh HPSV i.feritty wa not afecrcd !y irs lr..16. wnh dt.ctu pH of
Ad.novirus .xhihn a hi8h des.e ol r4isr.n@ to a vid. rans. of physicrl a.d
chemicalagenls, mos !!e sabl. on hating lo t6"C alrhough some isohtcs are inacrvared sr
5o'c (McFs.M .nd Adan, 1977) Fidis Eponr indi{r.d rh.r EDS vitu ina.crivity w.,
d.(rey.d o! h.lting it 0t 56"c for I hr (Ad.hriar, r9?9) Tne turt.y hodo.ihigic