Estudo do polimorfismo do sistema sangüíneo Diego em

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Exon 1 Anexo I o exon 1. Apêndice Seqüência DNA do gene SCL4A1 (GenBank: X77739). Em amarelo encontra-se 1 ggggggctgt ccgtgccccg tgagtgctga gcagttccct gtggagaaaa ctgcagagga 61 ggagtgagtc ctagcgtgga taccaagaga gacgagagga gcaggcgccc ttgcaccttg 121 gggccagagc agaagggcct ggccagagtg gaggatggac agaggcagga cagggacctc 181 tggaggctgg tgccttcctg ccagggcact gggagccctg aggacagagc gatcaaacct 241 tcatccacaa aggaagagtc aggagaacca tggggaccag gagggccccg agaggccttg 301 ggggctggcc aggcgggtgg ggtgggccag actcacagct gtccagatgt gggtaagaga 361 taactctgtt tactgggcac ccagtgggag cggggtgggg ggtgtgggaa ggggctccca 421 gggccagtgg ggcgggcaga ttaggaggcg ggggcatgag tcaggggttt gcgagctgcc 481 cctggggcgc tggtgctgtc agaacgagtg ggaacgtagc tggtcgcaga gggcaccagc 541 ggctgcagga cttcaccaag ggaccctgag gctcgtgagc aggtgggcag ggcagggagg 601 ggctggggat ggtccttcgg tgcctgccct gagggcttct acaggtgccc agcctgaatc 661 tatgcccagg tgggccaggc tcctgacctc caagggggtg caggtgggtg gggtgagaat 721 gttaccatgc atatgttgcc cctaagggaa caggcccatt ggcacctgaa aagaggaggg 781 tggcaggtgg tcaatggcag cagaggtatg tgtggctccg gtgcgtgtgt tgcggggggt 841 atctgatccc ctccaggtat gggcccttgt cagcttgttg gatcccacag ggtcaggtct 901 tgtccacccc agactccttc cttcctaatg gggtcctcag accaccccct ctcccaccta 961 ggaggcagga tgtcagctga cacagtatcc cctttgggac acttccaggg gtctacctgg 1021 ggcacctggc tccacgccca gcgtgtcagt attggaggga actgcagagc ctcctaccac 1081 atgagaatct caaaacactt agctcaggct aattccatgg gaactgaggg caaatcagag 1141 gggaaatgaa gatggcaagt ctgactgtaa ccccagcagc aagggataat gacagcaaac 1201 attttcatag cgctccttat atgctaggca ctgttctaag ccccttacaa atgtaaattt 1261 ccttaatgct tacaacaggt ctatgagatg ggcatcatta tcatcacccc cattttacag 1321 atgagaaaac tgaggcacag agagttgaag tcacacgccg ttagtaaatg gcagggctgg 1381 gctttgaatc caggcagtcc gactgcagag tccgtgatct taacaactaa ctctaaacca 1441 gctaacagtt gttttgttgg aggcaactta gcatagtgct tagggatgtg gctggagtca 1501 ggtagtccag gctgtaacct ggctctgata cctacctgct gggtggctga agcaggtaaa 1561 tgtctctgaa cctcagcttt cgcatctgta gaatggatta atgacattac tcttcttcct 1621 cttagaatcc ccaaaccact tcacagaatg ctttggggat tctaagagtt aaggcagtac 1681 agttctttgt gcagttcggg ctgattacac aaaggtggtg ccataccact aggaagttgt 1741 ccctgtcatc tggtcaatgc cctctcccgc cgtgtcttcc cagctgtgct ataccttcta 1801 ggctcagatg tcccgctcac ccttatctgg cctggcctgg cactggctag ctc 91
Exon 2 Exon 3 Exon 4 Exon 5 Exon 6 Anexo I - (Continuação) Apêndice Seqüência do DNA do gene SCL4A1 (GenBank: X77738).. Em amarelo, os exons. Em rosa, o desenho dos primers utilizados. 1 ggtgccagcc agacttacct cttccctgca ctgcccctct ctcccagccc ctgctcacct 61 accctcacac ctgcaaatcc agctcgttct ccctttccct ccacacttcc ttcctctggc 121 cctaccacct ccaacacgtg gatcccctcc actgacatgt ggccccctct ggaggaggac 181 caggagaccc tgggatgccc tgtgccccac tcagcctgtg ccctctgctg cagggacccg 241 cggtgcgggt tatgctgggg gctcagatca ccgtagacaa ctggacactc aggaccacgc 301 catggaggag ctgcaggtgg gtgttccctg gcatcagcag tgctcccacc tggaggctct 361 ggggacctgg tcctagactc ctatcttcgt tgggggtgga agagcccctc acagcccagc 421 ctcagcggcc acttcccaca ggatgattat gaagacatga tggaggagaa tctggagcag 481 gaggaatatg aagacccaga catccccgag tcccagatgg aggagccggc aggtgagccc 541 ctctccctcc ctcagctggg atgagctgct gagccctaga cagggacagg aggggcactt 601 gtcctctccc cttctccaca gtcctcctcc caagcaccac gggccgttct cccctcaatt 661 ctggctgatt gaaggataaa cagagctttg tttgaaatga tcacaggagg tggctgggag 721 agcaggggca gtcacccatc cagtgcccct gcaatgtgag aagggcgggc agccagtttg 781 ggacaagggc gtgagcccac atgccatctg tgcccatcca atgccagctt ccaggccaga 841 aaatgggctt gttcttgggg aaagaggtcc tgagaaatga ggggcttgtg gggaccctcc 901 tagatagaaa gacaccagga accaccctgc cttccctggc ctgtccaagg tcttaggtgg 961 ggagccccag gcaggacctg tttactgtca acaggactta agtgctggag attgggatta 1021 aagttaagag gcccctcttg gaaatgaggg ctgggggtgg gaggggtggg aatataaggg 1081 gctgacccac cctggtgcct cagctcagtg aaacactgtg ctgacgggct cattgaggca 1141 ggtgggagga gagaagggag tctggtctca gaggtggcag gggagggagg aggtcctagg 1201 aggatcctga aaattgccac tcagggctct gcagtcacct tggagaggtc ccttcacttc 1261 atcactgcgg agggctaggc ctccctggct gaatcccggg DiEX4S tgtcctcgac aaggctcaga 1321 tagggagccc aaggctaggc ccagcacaca agtgtgggga gcctcattct gctgagtggc 1381 cagtgtggga tgcggcacca gagacccagc ccagggcact gggaaggagg ggcatctgcc 1441 catgcctggg tctctgaggc tcacagtgga tggtgaagag cctcaggtgg gggacggtca 1501 ggtcaatact aaccgacctc tggttttcag ctcacgacac cgaggcaaca gccacagact 1561 accacaccac atcacacccg ggtacccaca aggtgaggac cccagcctcc tccgttcttt 1621 cccatgctgc ctttgggacc caccatttga tcagggaaga gaggagcaag gggatagagg 1681 cagcttcagg ctgggggaga ccctgcagca gatagaggag tgaggatccc aggaaacagg 1741 tgggagggac gccccactga tggggaggcc tgggcccctt tccaggagca ccctgggcag DiI4AS 1801 agataacctg attgacaagg gctgagtccc caggccctca ggccctggct gggctgtcgg 1861 gcgtcactgg ggaagcacag aattaaagac ttgttctcct ctgcccaggc ccctcaggtc 1921 cccactgggc actgaactgt ctaagtggtt agaaatttgg ggtggccctc agctatggcc 1981 cttggagcaa ccacctggcc tcagggacat gctaaagcac atccacgaac aatcttagag 2041 gctggagact gacccaatga ctccaaagtc cctttagctt cggcccgcca ggcctctgct 2101 tcccactcct ttccctcccc agtggcctct ccagcatcct ttgggtcagg gttctccaaa 2161 tgccttctgc aatggggtaa aggcctggcc aggaagttgg gagataaggg agtggtgtga 2221 gccgtatcta cggacccttg agcacaggga tccaagcctc acaagcacaa gcctctctcg 2281 agagtggctg agcacccact atgccctgga ctatgcctga tgaactaact caggcgcatg 2341 gggccacagg tctatgtgga gctgcaggag ctggtgatgg acgaaaagaa ccaggagctg 2401 agatggatgg aggcggcgcg ctgggtgcaa ctggaggaga acctggggga gaatggggcc 2461 tggggccgcc cgcacctctc tcacctcacc ttctggagcc tcctagagct gcgtagagtc 2521 ttcaccaagg gtgagcctcc tgggcccttc tggaccccct gcagatgagg attgttgtgg 2581 ggtgctgtgg cccagcctac cagctcactc ctccactgcc tgcaggtact gtcctcctag 2641 acctgcaaga gacctccctg gctggagtgg ccaaccaact gctagacagg tttatctttg 2701 aagaccagat ccggcctcag gaccgagagg agctgctccg ggccctgctg cttaaacaca 2761 ggtgcccctg cctgccaggg cctggattcc atatcccagt gggcccactt ccccagccca 2821 cttgccccaa gttctccccc gggatccagg tggccctacc ctcaccaggc aggggcccac 2881 tttcctccac tatggctccc atctctgaca ccttgaaagg ggtggccatg tcctatgctc 2941 agagttgggg gctgttggtc cccaggaggg cagacctgca aggccctttt ctgtagccac 3001 tcttgatccc gcctcgtcat tccagaggcc acccacttac atctacaacg tccttggaga 92
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ESTUDO DO POLIMORFISMO DO SISTEMA S
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Agradeço especialmente às “meni
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Agradeço algumas pessoas pela imen
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Agradecimento ao meu marido: com qu
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Resumo Estudo do Polimorfismo do Si
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SUMMARY
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Summary studied. The DI A allele ha
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5.7.2- PCR exon 4 38 5.8- Reação
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3- Introdução Introdução Até 1
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Introdução Figura 2: Representaç
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Introdução tetrâmeros Rh e podem
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Introdução (ZELINSKI, 1993). Info
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Introdução asiáticos (chineses e
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Ocorrência do genótipo Wr a Wr a
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Introdução por polimorfismos úni
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Introdução Desta forma foram incl
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Exon 11 Exon 12 Exon 13 Exon 14 Int
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Exon 19 Exon 20 Introdução 803 At
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OBJETIVOS
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MATERIAL E MÉTODOS
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Material e Métodos Os candidatos f
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5.4- Fenotipagem Eritrocitária Mat
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Colorado, USA), de acordo com a esp
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Material e Métodos Em seguida a re
Page 49 and 50: 14 50 54 70 86 139 27 74 127 40 101
Page 51 and 52: Material e Métodos Figura 19: Foto
Page 53 and 54: Material e Métodos condições: 1,
Page 55 and 56: Material e Métodos polimorfismo a
Page 57 and 58: RESULTADOS
Page 59 and 60: Resultados Figura 25: Eletroferogra
Page 61 and 62: Resultados amostras (THE HUMAN...).
Page 63 and 64: Resultados Figura 31: Eletroferogra
Page 65 and 66: Resultados Figura 35: Representaç
Page 67 and 68: Resultados Tabela 13: Relação das
Page 69 and 70: Resultados reconhece o sítio de re
Page 71 and 72: Resultados Tabela 15: Relação das
Page 73 and 74: Posição 1.314 do gene (exon 12):
Page 75 and 76: 7- Discussão Discussão Este estud
Page 77 and 78: Discussão As demais populações e
Page 79 and 80: Discussão que o índice de alosens
Page 81 and 82: Discussão se faz necessária quand
Page 83 and 84: Discussão em 2002 (1,2%) (tabela 2
Page 85 and 86: Discussão (BALEOTTI, 2002). Avalia
Page 87 and 88: Discussão Figura 41: Tronco Macro-
Page 89 and 90: Discussão estudas. Nosso resultado
Page 91 and 92: 8.Conclusões Conclusões O gene qu
Page 93 and 94: REFERÊNCIAS BIBLIOGRÁFICAS
Page 95 and 96: Newborn Attribulatable to Anti-Wr a
Page 97 and 98: http://www.socioambiental.org/websi
Page 99: APÊNDICE
Page 103 and 104: Exon 14 Exon 15 Exon 16 Anexo I - (
Page 105 and 106: Exon 20 Anexo I - (Continuação) A
Page 107 and 108: Anexo II FUNDAÇÃO HEMOCENTRO DE R
Page 109 and 110: Anexo III Apêndice Alinhamento das
Page 111 and 112: Anexo III (Continuação) Apêndice
Page 117 and 118: Anexo III (continuação) Apêndice
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Anexo III (Continuação) Apêndice
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Anexo IV Apêndice Relação das am
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Anexo IV Relação das amostras que
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